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CTBS Practice Exam 2 (2025/2026) – Actual Questions and Answers | Latest Updated Version (Graded A+)

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This document provides the CTBS Practice Exam 2 for the 2025/2026 academic year, featuring real exam questions with verified correct answers. It includes the latest updates and is graded A+, offering a reliable and comprehensive resource for preparation. Ideal for students and professionals seeking to master the Certified Tissue Bank Specialist (CTBS) exam through accurate, up-to-date practice materials that reflect the current testing standards.

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CTBS Practice Exam 2 2024/2025 | Actual Questions and Answers Latest
Updated 2024/2025 (Graded A+)


You are responsible for Quality checking residual chemical levels and process bone grafts and a very
small piece of tissue(<100 mg), the residual ethylene glycol level can be up to blank parts per million.

A. 250

B. 2000

C. 2500

D. 5000 - ✔✔D.5000



You are quality checking microbiological results on a heart valve-graft. They found clostridium
perfringens on the tissue. This microbe will result in automatic discard of the tissue:

A. true, Clostridium species is not allowed.

B. true, but only because of my medical director

C. False, clostridium can be irradiated.

D. False, clostridium can be removed with antibiotics. - ✔✔A. True, Clostridium species is not allowed.



Fresh or cryopreserved skin shall not be used if any of the following organisms are found. -
✔✔Staphylococcus aureus, Group A strep(S. pyogenes), Enterococcus species, Gram negative bacilli,
Clostridium(genus), Fungi(yeasts & molds), fast growing fungal organisms.



History of tissue banking Who started and in what year? - ✔✔U.S. Navy Tissue Bank in 1949



What year did AATB start? - ✔✔1976



What year were 1st standards created by AATB? - ✔✔1984



You work for an organization known as a Tissue Dispensing Service. Who is responsible to supervise your
organization's activities? - ✔✔A Physician

,You are training a staff member about authorization and they ask you: What makes a Document of
Authorization different than a Document of Gift?

A. It is a legal record

B. It describes postmortem recovery

C. It can be signed or recorded.

D. The signer is an Authorizing person. - ✔✔D. The signer is a Authorizing person.



You are a recovery coordinator reviewing a donor's fluid intake. At what age must you have blood loss
and infusion of >2000 ml of blood products/fluids when considering plasma dilution?

A. >12 years old or = too

B. =13 years of age et older

C. < or = to 12 years old

D. < 12 years old. - ✔✔B.

=13 years of age et older.



Cardiac, Vascular

Storage conditions and temperature (C)

A. lyophilized & Ambient

B. Frozen, cryopreserved -40 C or colder

C. Frozen, cryopreserved, -100 C or colder

D. Frozen, cryopreserved & -20c to -40c - ✔✔C. Frozen cryopreserved & -100 C or colder.



For Skin, What is the correct storage conditions and temperature (C)

A. Frozen, cryopreserved & LN2(Liquid Vapor Phase)

B. Refrigerated and above freezing(0 degrees Celsius) to 10 degree Celsius.

C. Frozen, Cryopreserved & -100 Celsius or colder.

D. lyophilized & Ambient - ✔✔B. Refrigerated and above freezing to 10 degrees celcius

D. lyophilized & Ambient

, What is the name of the valve between the left atrium and left ventricle?

A. Tricuspid valve

B. Mitral Valve

C. Bicuspid valve

D. Pulmonic valve - ✔✔B. Mitral valve and also known as bicuspid valve



Chagas' disease is caused by what parasite?

A. T. brucei

B. T. Cruzi

C. T. Avium

D. T. Lewisi - ✔✔B. T Cruzi



When handling sterile drapes, you must be sure that the drape is presented: - ✔✔To a sterile person
who is scrubbed, gowned, & gloved.



The Achilles bone is attached to which bone? - ✔✔Calcaneus



Refrigerated skin may be returned to inventory if it has been maintained in a closed container at: - ✔✔1-
10 degrees Celsius



The most accurate way to test for the presence of contaminating of tissue allografts is by: - ✔✔Culture
of blood and of the excised tissue of the donor at the time of recovery.



What are the 3 mechanisms of bone healing? - ✔✔Osteoconductivity

Osteoinductivity

Osteogenicity



What is Osteconduction? - ✔✔The process population of bone cells of the host into an inert graft.

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