Microbiology Lab Final - GCSU Bachoon Exam |160
Questions and Answers
microbes - -According to health care experts, infectious diseases caused by ______________ are
responsible for more deaths worldwide than any other single cause.
-contaminants - -Adequate precautions are necessary to prevent unwanted _________________
from getting in or on experimental tubes or plates.
-Flame the inoculating loop - -What is the first step of aseptic technique?
-mix up organisms in culture - -What is the second step of aseptic technique?
-flame the mouth of the tube - -What is the third step of aseptic technique?
-remove organisms from tube using the inoculating loop. - -What is the fourth step of
aseptic technique?
-remove loop from culture and flame the mouth of the tube and close the tube - -What is
the fifth step of aseptic technique?
-transfer the loopful of bacteria to a test tube or Petri plate - -What is the 6th step of
aseptic technique?
-After transferring, flame the loop - -What is the seventh step of aseptic technique?
-E. coli - -What bacteria did we use in the Aseptic Technique lab (Exercise 2)?
-Base - -used in carrying the microscope; houses the light source
-light source - -provides illumination to specimen
-arm - -along with the base, used as a carrying handle for the microscope
-stage - -platform which the specimen rests on
-course and fine adjustment knobs - -controls the distance between the specimen and the
stage
-coarse focus - -is used with scan and low power objective lenses
-fine focus - -is used with high dry and oil immersion objective lenses
-mechanical stage adjusters - -allow the movement of the stage from left to right and
forward to backward
, -condenser - -collects and concentrates light emitted from the light sources into a tight
beam before passing it through the specimen
-iris diaphragm - -located within the condenser; tightens or widens the beam of light
emitted from the condenser
-light intensity adjuster - -controls the amount of light emitted from the source bulb
-objective lenses - -serve to magnify the specimen in part
-scanning power - -4x magnification
-low power - -10x magnification
-high dry power - -40x magnification
-oil immersion - -100x magnification
-revolving nosepiece - -allows the parfocal exchange between objective lenses during
specimen magnification
-ocular lens - -allows the image to be viewed; magnifies the specimen 15x; may be
monocular or binocular
-refraction of light - -__________________ is minimized by the proper adjustment of the
microscope parts and through the use of immersion oil.
-high - -The condenser should be raised as _____________ as possible to minimize loss of light
as the rays pass from the glass of the lens to the glass of the slide on which specimens are
mounted.
-wipe oil off of the microscope - -What do you do after using the oil immersion lens?
-red - -Gram negative cells stain ________________.
-purple - -Gram positive cells stain ________________.
-Crystal Violet - -What is the first stain used in a Gram stain?
-To impart color to all organisms on the smear - -What is the purpose of Crystal Violet?
-Gram's Iodine - -What is the second reagent used in a Gram stain?
-mordant - -Gram's iodine acts as a _________________.
Questions and Answers
microbes - -According to health care experts, infectious diseases caused by ______________ are
responsible for more deaths worldwide than any other single cause.
-contaminants - -Adequate precautions are necessary to prevent unwanted _________________
from getting in or on experimental tubes or plates.
-Flame the inoculating loop - -What is the first step of aseptic technique?
-mix up organisms in culture - -What is the second step of aseptic technique?
-flame the mouth of the tube - -What is the third step of aseptic technique?
-remove organisms from tube using the inoculating loop. - -What is the fourth step of
aseptic technique?
-remove loop from culture and flame the mouth of the tube and close the tube - -What is
the fifth step of aseptic technique?
-transfer the loopful of bacteria to a test tube or Petri plate - -What is the 6th step of
aseptic technique?
-After transferring, flame the loop - -What is the seventh step of aseptic technique?
-E. coli - -What bacteria did we use in the Aseptic Technique lab (Exercise 2)?
-Base - -used in carrying the microscope; houses the light source
-light source - -provides illumination to specimen
-arm - -along with the base, used as a carrying handle for the microscope
-stage - -platform which the specimen rests on
-course and fine adjustment knobs - -controls the distance between the specimen and the
stage
-coarse focus - -is used with scan and low power objective lenses
-fine focus - -is used with high dry and oil immersion objective lenses
-mechanical stage adjusters - -allow the movement of the stage from left to right and
forward to backward
, -condenser - -collects and concentrates light emitted from the light sources into a tight
beam before passing it through the specimen
-iris diaphragm - -located within the condenser; tightens or widens the beam of light
emitted from the condenser
-light intensity adjuster - -controls the amount of light emitted from the source bulb
-objective lenses - -serve to magnify the specimen in part
-scanning power - -4x magnification
-low power - -10x magnification
-high dry power - -40x magnification
-oil immersion - -100x magnification
-revolving nosepiece - -allows the parfocal exchange between objective lenses during
specimen magnification
-ocular lens - -allows the image to be viewed; magnifies the specimen 15x; may be
monocular or binocular
-refraction of light - -__________________ is minimized by the proper adjustment of the
microscope parts and through the use of immersion oil.
-high - -The condenser should be raised as _____________ as possible to minimize loss of light
as the rays pass from the glass of the lens to the glass of the slide on which specimens are
mounted.
-wipe oil off of the microscope - -What do you do after using the oil immersion lens?
-red - -Gram negative cells stain ________________.
-purple - -Gram positive cells stain ________________.
-Crystal Violet - -What is the first stain used in a Gram stain?
-To impart color to all organisms on the smear - -What is the purpose of Crystal Violet?
-Gram's Iodine - -What is the second reagent used in a Gram stain?
-mordant - -Gram's iodine acts as a _________________.
