bachoon lab final Exam |130 Questions and Answers
aseptic technique - -technique designed to keep unwanted microorganisms from
contaminating sterile materials
-what type of microbes dominated in the air of your microbiology lab? - -microorganisms
-why is it important to use aseptic technique when inoculating tubes and media plates? - -
to prevent unwanted organisms (contaminants) from getting in or on experimental tubes
or plates.
-TM - -total magnification
-base of microscope - -used in carrying the microscope; houses the light source
-light source of microscope - -provides illumination to specimen
-arm of microscope - -along with base, used as a carrying handle for the microscope
-stage of microscope - -platform which the specimen rests on
-coarse and fine focus adjustment knobs - -controls the distance between the specimen
and the stage; coarse focus is used with scan and low power objective lenses; fine focus is
used with high dry and oil immersion objective lens
-mechanical stage adjusters of microscope - -allows the movement of the stage from left to
right and forward to backward
-condenser of microscope - -collects and concentrates light emitted from the light sources
into a tight beam before passing it through the specimen
-iris diaphragm of microscope - -located within the condenser; tightens or widens the
beam of light emitted from the condenser
-light intensity adjuster of microscope - -controls the amount of light emitted from the
source bulb
-objective lenses of microscope - -serve to magnify the specimen in part; scan, low power,
high dry power, oil immersion
-scan objective lens - -4x magnification
-low power objective lens - -10x magnification
-high dry power objective lens - -40x magnification
, -oil immersion objective lens - -100x magnification
-revolving nosepiece of microscope - -allows the parfocal exchange between objective
lenses during specimen magnification
-ocular lens of microscope - -allows the image to be viewed; magnifies the specimen 10x;
may be monocular or binocular
-how does the procedure for using the microscope differ using high dry power as opposed
to low power? - -high power is used for smaller objects and uses fine focus lens, low power
is used for larger objects and uses coarse focus lens
-how does loss of light affect the resolution of a specimen? - -decreases the resolution or
clarity
-what is the purpose of using immersion oil? - -refraction of light is minimized and
increases the resolution
-what two things can be adjusted to control the amount of light passing into a specimen? -
-iris diaphragm and light intensity adjuster
-describe how to properly care for a microscope after immersion oil has been used. - -
remove oil with isopropanol alcohol and lens paper
-microscopy can be used to - -identify whether bacteria are present in otherwise sterile
body fluids
-two common dyes used in the lab - -acidic (anionic) dyes and basic (cationic) dyes
-_______________ simple stains are used to color the cells and leave the background colorless -
-positive
-______________ simple stains are different in that the cells remain colorless and the
background retains the stain - -negative
-three morphologies of bacteria - -coccus, bacillus, and spirillum
-coccus - -spherical; plural: cocci
-bacillus - -rod; plural: bacilli
-spirillum - -corkscrew; plural: spirilla
-diplo- - -pair of cocci or bacilli
aseptic technique - -technique designed to keep unwanted microorganisms from
contaminating sterile materials
-what type of microbes dominated in the air of your microbiology lab? - -microorganisms
-why is it important to use aseptic technique when inoculating tubes and media plates? - -
to prevent unwanted organisms (contaminants) from getting in or on experimental tubes
or plates.
-TM - -total magnification
-base of microscope - -used in carrying the microscope; houses the light source
-light source of microscope - -provides illumination to specimen
-arm of microscope - -along with base, used as a carrying handle for the microscope
-stage of microscope - -platform which the specimen rests on
-coarse and fine focus adjustment knobs - -controls the distance between the specimen
and the stage; coarse focus is used with scan and low power objective lenses; fine focus is
used with high dry and oil immersion objective lens
-mechanical stage adjusters of microscope - -allows the movement of the stage from left to
right and forward to backward
-condenser of microscope - -collects and concentrates light emitted from the light sources
into a tight beam before passing it through the specimen
-iris diaphragm of microscope - -located within the condenser; tightens or widens the
beam of light emitted from the condenser
-light intensity adjuster of microscope - -controls the amount of light emitted from the
source bulb
-objective lenses of microscope - -serve to magnify the specimen in part; scan, low power,
high dry power, oil immersion
-scan objective lens - -4x magnification
-low power objective lens - -10x magnification
-high dry power objective lens - -40x magnification
, -oil immersion objective lens - -100x magnification
-revolving nosepiece of microscope - -allows the parfocal exchange between objective
lenses during specimen magnification
-ocular lens of microscope - -allows the image to be viewed; magnifies the specimen 10x;
may be monocular or binocular
-how does the procedure for using the microscope differ using high dry power as opposed
to low power? - -high power is used for smaller objects and uses fine focus lens, low power
is used for larger objects and uses coarse focus lens
-how does loss of light affect the resolution of a specimen? - -decreases the resolution or
clarity
-what is the purpose of using immersion oil? - -refraction of light is minimized and
increases the resolution
-what two things can be adjusted to control the amount of light passing into a specimen? -
-iris diaphragm and light intensity adjuster
-describe how to properly care for a microscope after immersion oil has been used. - -
remove oil with isopropanol alcohol and lens paper
-microscopy can be used to - -identify whether bacteria are present in otherwise sterile
body fluids
-two common dyes used in the lab - -acidic (anionic) dyes and basic (cationic) dyes
-_______________ simple stains are used to color the cells and leave the background colorless -
-positive
-______________ simple stains are different in that the cells remain colorless and the
background retains the stain - -negative
-three morphologies of bacteria - -coccus, bacillus, and spirillum
-coccus - -spherical; plural: cocci
-bacillus - -rod; plural: bacilli
-spirillum - -corkscrew; plural: spirilla
-diplo- - -pair of cocci or bacilli
