Microbiology Lab Final (Bachoon-GCSU)|120
Questions and Answers
Disinfect the lab table - -What's the first thing you do when you walk into the lab?
-before AND after lab - -When do you wash your hands?
-label - -ALWAYS _____ the bottom of the dishes
-flaming (heating until red hot) - -Inoculating loop or needle is sterilized by
-contaminated - -Before you get a microbe from the tube you must heat up your
inoculating loop, then put the tube over the fire, dip the loop in, then put the tube back over
the fire. This is done to keep anything from becoming
-To prevent contamination of cultures and media from microbes in the environment - -
Why is it important to use aseptic techniques when inoculating tubes and media plates
-Base function - -Used in carrying the microscope; houses the light source
-base - -what is the red arrow pointing to?
-Light source function - -Provides illumination to specimen
-light source - -what is the red arrow pointing to?
-Arm function - -Along with the base, used as a carrying handle for the microscope
-arm - -what is the red arrow pointing to?
-Stage function - -Platform which the specimen rests on
-stage - -what is the red arrow pointing to?
-Coarse and fine focus adjustment knobs function - -Controls the distance between the
specimen and the stage; coarse focus is used with scan and low power objective lenses; fine
focus is used with high dry and oil immersion objective lenses
-course focus adjustment knob - -what is the red arrow pointing to?
-fine focus adjustment knob - -what is the red arrow pointing to?
-mechanical stage adjusters - -allow the movement of the stage from left to right and
forward to backward
, -mechanical stage adjusters - -what is the pink arrow pointing to?
-condensor - -collects and concentrates light emitted from the light sources into a tight
beam before passing it through the specimen
-condensor - -what is the red arrow pointing to?
-iris diaphragm - -located within the condenser; tightens or widens the beam of light
emitted from the condenser
-iris diaphragm - -what is the black arrow pointing to?
-light intensity adjuster - -controls the amount of light emitted from the source bulb
-light intensity adjuster - -what is the red arrow pointing to?
-objective lenses - -serve to magnify the specimen in part
-objective lenses - -what is the red arrow pointing to?
-cerebrospinal fluid; meningitis - -Where is the human body devoid of bacterial flora
-4x magnification - -Scan power with objective lenses
-10x magnification - -Low power with the objective lenses
-40x magnification - -High dry power with the objective lenses
-100x magnification - -Oil immersion with the objective lenses
-Never use coarse adjustment knob on high power only the fine adjustment - -How does
the procedure for using the microscope differ using high power as opposed to low power
-increase the resolving power of a microscope - -What is the purpose of using immersion
oil?
-anionic (negative) - -Acidic dyes are called
-cationic (positive) - -Basic dyes are called
-Steps to take BEFORE staining - -When first staining cells you must smear the cells and
allow them to air dry
Once they are air dried, you must flame the cells (fixation) this keeps them from sliding off
or doing anything crazy
Then you can start staining
Questions and Answers
Disinfect the lab table - -What's the first thing you do when you walk into the lab?
-before AND after lab - -When do you wash your hands?
-label - -ALWAYS _____ the bottom of the dishes
-flaming (heating until red hot) - -Inoculating loop or needle is sterilized by
-contaminated - -Before you get a microbe from the tube you must heat up your
inoculating loop, then put the tube over the fire, dip the loop in, then put the tube back over
the fire. This is done to keep anything from becoming
-To prevent contamination of cultures and media from microbes in the environment - -
Why is it important to use aseptic techniques when inoculating tubes and media plates
-Base function - -Used in carrying the microscope; houses the light source
-base - -what is the red arrow pointing to?
-Light source function - -Provides illumination to specimen
-light source - -what is the red arrow pointing to?
-Arm function - -Along with the base, used as a carrying handle for the microscope
-arm - -what is the red arrow pointing to?
-Stage function - -Platform which the specimen rests on
-stage - -what is the red arrow pointing to?
-Coarse and fine focus adjustment knobs function - -Controls the distance between the
specimen and the stage; coarse focus is used with scan and low power objective lenses; fine
focus is used with high dry and oil immersion objective lenses
-course focus adjustment knob - -what is the red arrow pointing to?
-fine focus adjustment knob - -what is the red arrow pointing to?
-mechanical stage adjusters - -allow the movement of the stage from left to right and
forward to backward
, -mechanical stage adjusters - -what is the pink arrow pointing to?
-condensor - -collects and concentrates light emitted from the light sources into a tight
beam before passing it through the specimen
-condensor - -what is the red arrow pointing to?
-iris diaphragm - -located within the condenser; tightens or widens the beam of light
emitted from the condenser
-iris diaphragm - -what is the black arrow pointing to?
-light intensity adjuster - -controls the amount of light emitted from the source bulb
-light intensity adjuster - -what is the red arrow pointing to?
-objective lenses - -serve to magnify the specimen in part
-objective lenses - -what is the red arrow pointing to?
-cerebrospinal fluid; meningitis - -Where is the human body devoid of bacterial flora
-4x magnification - -Scan power with objective lenses
-10x magnification - -Low power with the objective lenses
-40x magnification - -High dry power with the objective lenses
-100x magnification - -Oil immersion with the objective lenses
-Never use coarse adjustment knob on high power only the fine adjustment - -How does
the procedure for using the microscope differ using high power as opposed to low power
-increase the resolving power of a microscope - -What is the purpose of using immersion
oil?
-anionic (negative) - -Acidic dyes are called
-cationic (positive) - -Basic dyes are called
-Steps to take BEFORE staining - -When first staining cells you must smear the cells and
allow them to air dry
Once they are air dried, you must flame the cells (fixation) this keeps them from sliding off
or doing anything crazy
Then you can start staining
