Portage Microbiology BIOD171 Exam 5
Define the concept of Universal precautions means any and all samples, whether
universal precautions. known or unknown, are to be treated as potentially
hazardous (or pathogenic) materials.
A lab researcher would be certain to note:
Size and shape
List at least 3 observations a
Any observable motility
researcher would be sure to
Gram status (positive or negative)
note while assessing an
The presence of any chemical reactions
unknown microbial sample. Changes in color localized to the organism or
the surrounding media Capture (or draw)
images of any of the characteristics described
above
While observing an unknown You must determine motility before determining the
sample of limited amounts, a Gram status. Motility requires a wet mount, while Gram
researcher must determine the staining requires heat fixing the sample. If one were to
following observations: (1) the begin with the Gram stain the heat fixation process
presence of any motility and would kill the organism, making any observations
(2) its Gram status using the regarding motility impossible. The correct approach
same sample—the liquid would be to place the liquid culture on a glass slide and
sample cannot be divided. determine its motility status. Next, the same liquid
Which would you determine culture can be heat fixed and Gram stained.
first and why?
A facultative anaerobe is a A facultative anaerobe is capable of growth under
microorganism capable of aerobic (with oxygen) and anaerobic (without oxygen)
growth under what conditions.
conditions?
As Streptococcus is Streptococcus is catalse negative which means it cannot
catalase negative would it break down peroxides and if any peroxides are present it
thrive or die in the would die.
presence of peroxides?
Why?
, Blood agar
Streptococcus is most often
streaked onto:
Strep is often cultured on Blood agar plates to determine its
hemolytic properties, which aids in the classification (and
differentiation) process.
True or False. The False
Lancefield groups are used
to subdivide antigenic The Lancefield groupings are used to subdivide beta-hemolytic
Strep.
groups of gamma-
hemolytic Streptococcus.
The distinctions for Carbohydrate composition of antigens
Lancefield subgroupings lie in
its: (select all that apply)
Carbohydrate composition of antigens present in the cell
Catalase wall determines the Lancefield groupings (A, B, C, etc).
activity Note, ALL strep under Lancefield groupings are (by
Hemolytic definition) catalase negative and beta-hemolytic. Thus,
activity answers A and C cannot be used to subdivide
Carbohydrate composition streptococcal species.
of antigens All of the
above
Left untreated, strep throat can Rheumatic fever; beta
progress to
______ , which displays_hemolytic
activity.
True or False. Under most false; While staphylococcus can be found in ~30% of the
circumstances, human population, it remains non-symptomatic.
staphylococcus can be found
in ~30% of human population
where it remains pathogenic.
When a bacterium neither Commensal
harms nor benefits from the
host from which it obtains
nutrients, it is referred to as
being_________________________.
Attachment pathogen finds a specific target tissue and adhere
evasion pathogen masks its own antigens to hide from the host's immune
system
entry utilization of a portal to gain access to host's tissues
Match the following diseases 1. D
with their respective 2. E
symptoms: 3. A
1. folliculitis 4. B
2. scalded-skin syndrome 5. C
3. impetigo
4. conjunctivitis
Define the concept of Universal precautions means any and all samples, whether
universal precautions. known or unknown, are to be treated as potentially
hazardous (or pathogenic) materials.
A lab researcher would be certain to note:
Size and shape
List at least 3 observations a
Any observable motility
researcher would be sure to
Gram status (positive or negative)
note while assessing an
The presence of any chemical reactions
unknown microbial sample. Changes in color localized to the organism or
the surrounding media Capture (or draw)
images of any of the characteristics described
above
While observing an unknown You must determine motility before determining the
sample of limited amounts, a Gram status. Motility requires a wet mount, while Gram
researcher must determine the staining requires heat fixing the sample. If one were to
following observations: (1) the begin with the Gram stain the heat fixation process
presence of any motility and would kill the organism, making any observations
(2) its Gram status using the regarding motility impossible. The correct approach
same sample—the liquid would be to place the liquid culture on a glass slide and
sample cannot be divided. determine its motility status. Next, the same liquid
Which would you determine culture can be heat fixed and Gram stained.
first and why?
A facultative anaerobe is a A facultative anaerobe is capable of growth under
microorganism capable of aerobic (with oxygen) and anaerobic (without oxygen)
growth under what conditions.
conditions?
As Streptococcus is Streptococcus is catalse negative which means it cannot
catalase negative would it break down peroxides and if any peroxides are present it
thrive or die in the would die.
presence of peroxides?
Why?
, Blood agar
Streptococcus is most often
streaked onto:
Strep is often cultured on Blood agar plates to determine its
hemolytic properties, which aids in the classification (and
differentiation) process.
True or False. The False
Lancefield groups are used
to subdivide antigenic The Lancefield groupings are used to subdivide beta-hemolytic
Strep.
groups of gamma-
hemolytic Streptococcus.
The distinctions for Carbohydrate composition of antigens
Lancefield subgroupings lie in
its: (select all that apply)
Carbohydrate composition of antigens present in the cell
Catalase wall determines the Lancefield groupings (A, B, C, etc).
activity Note, ALL strep under Lancefield groupings are (by
Hemolytic definition) catalase negative and beta-hemolytic. Thus,
activity answers A and C cannot be used to subdivide
Carbohydrate composition streptococcal species.
of antigens All of the
above
Left untreated, strep throat can Rheumatic fever; beta
progress to
______ , which displays_hemolytic
activity.
True or False. Under most false; While staphylococcus can be found in ~30% of the
circumstances, human population, it remains non-symptomatic.
staphylococcus can be found
in ~30% of human population
where it remains pathogenic.
When a bacterium neither Commensal
harms nor benefits from the
host from which it obtains
nutrients, it is referred to as
being_________________________.
Attachment pathogen finds a specific target tissue and adhere
evasion pathogen masks its own antigens to hide from the host's immune
system
entry utilization of a portal to gain access to host's tissues
Match the following diseases 1. D
with their respective 2. E
symptoms: 3. A
1. folliculitis 4. B
2. scalded-skin syndrome 5. C
3. impetigo
4. conjunctivitis
