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QUESTIONS ANSWERS
1. Mothers pass immunity to offspring
Paul Ehrlich's 3 rules about immunity 2. Fathers do not pass immunity to offspring
3.Transferring serum transfers (short-lived) immunity
Ratio of products when antibody is 2:1 ratio of F_ab to F_c
digested with papain
Ratio of products when disulfide bonds of 1:1 heavy (IgH) to light (IgL) chain
antibodies are reduced
What do goats immunized with rabbit F_ab IgH and IgL
recognize?
What do goats immunized with rabbit F_c IgH only
recognize
What is the difference between a B cell BCR is membrane bound
receptor and an antibody?
Both
Similarities between BCR and Ab (from the same B cell)
immunoglobul
ins Same amino
acid sequence
Same
specificity
What are the associated signaling Igα and Igβ
domains for BCRs?
What do Igα and Igβ signal through? Intracellular tyrosine activation motifs (ITAM)
ITAM intracellular tyrosine activation motifs
CR2
What is the B cell co-receptor, which
Made of
two proteins make it up, and what does
CD21 and
it bind?
CD19
Binds iC3b
bound to
antigen
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, Basic composition of BCR/Ab 2 identical heavy chains (IgH) + 2 identical light chains (IgL)
What are the two known light chains? Igκ and Igλ
Which chain contains the transmembrane Heavy chain (IgH)
region anchoring a BCR into the cell
membrane?
How are the two heavy chains linked Intermolecular disulfide bonds in the "hinge" region
together?
How are the two light chains linked Intermolecular disulfide bonds
together?
How many disulfide bonds are present 16
in an antibody?
How many domains compose the 4-5 domains
heavy chain and how many of these 1 variable (V_H) and 3-4 constant (C_H1-C_H4)
domains are variable/constant?
How many domains do light chains 2 domains, 1 V_L and 1 C_L
have and how many of these are
variable/constant?
Which domains make up the variable domains
antigen binding region?
Determines:
What is the function of the constant domain? membrane
vs
secreted
isotype
function
BCRs are divalent
Are BCRs/Abs monovalent, divalent, etc.?
Abs may have higher valency (up to 10)
Describe the immunoglobulin "Immunoglobulin taco"
characteristic folding pattern 2 β-pleated sheets folded on top of each other
How are the two β-pleated sheets held Disulfide (cysteine) bond
together in an Ig domain?
Which portion of the antibody recognizes Variable domain
antigen?
How do you determine which region of an Find the 3 hypervariable regions (HVRs) of variable
antibody are the complementarity domain
determining regions (CDRs)?
How do you determine which portion of an Compare amino acid sequences across many different
anitbody are the hypervariable regions antibodies
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