Bio 200 CURE Prelab 5
Rifampicin resistant bacteria can survive in a growth media containing the drug
rifampicin? - answer True
Rifampicin sensitive bacteria can survive in a growth media containing the drug
rifampicin? - answer False
What do we mean by, "progenitor" in this experiment? - answer a bacteria that was
originally exposed to Rifampicin and survived.
What do we mean by, "descendant" in this experiment? - answera bacteria that was
exposed to Rifampicin, and was then grown in a media without Rifampicin for a series of
generations.
What makes the RNA Polymerase from a sensitive bacterial strain different from the
RNA Polymerase from a resistant strain? - answer-DNA sequence
-rpoB DNA sequence
-Primary protein structure of the beta subunit of RNA Polymerase
-mRNA for the rpoB gene
The DNA of the rpoB gene in the sensitive strain is ________ the DNA of the rpoB gene
in the resistant strain. - answerdifferent than
Why is the resistant bacteria resistant to Rifampicin? - answerthe rpoB gene has
changed, as a result the protein has changed, as a result Rifampicin can no longer bind
at the typical binding site. The end result is normal transcription
What color will the following nucleotides be in a chromatogram? - answer-G = Black
-T = Red
-A = Green
-C = Blue
-U = Not used in Sanger sequence
What can you tell about the quality of information based on the provided
chromatogram? - answerThis chromatogram has a lot of "noise" and is likely an
unreliable sequence
Sequence information at the ends of the sequenced DNA: - answeris often unreliable
and should not be read
How much of the sequenced DNA is believed to be accurate? - answer500 -800 base
pairs in the middle of the sequence
Rifampicin resistant bacteria can survive in a growth media containing the drug
rifampicin? - answer True
Rifampicin sensitive bacteria can survive in a growth media containing the drug
rifampicin? - answer False
What do we mean by, "progenitor" in this experiment? - answer a bacteria that was
originally exposed to Rifampicin and survived.
What do we mean by, "descendant" in this experiment? - answera bacteria that was
exposed to Rifampicin, and was then grown in a media without Rifampicin for a series of
generations.
What makes the RNA Polymerase from a sensitive bacterial strain different from the
RNA Polymerase from a resistant strain? - answer-DNA sequence
-rpoB DNA sequence
-Primary protein structure of the beta subunit of RNA Polymerase
-mRNA for the rpoB gene
The DNA of the rpoB gene in the sensitive strain is ________ the DNA of the rpoB gene
in the resistant strain. - answerdifferent than
Why is the resistant bacteria resistant to Rifampicin? - answerthe rpoB gene has
changed, as a result the protein has changed, as a result Rifampicin can no longer bind
at the typical binding site. The end result is normal transcription
What color will the following nucleotides be in a chromatogram? - answer-G = Black
-T = Red
-A = Green
-C = Blue
-U = Not used in Sanger sequence
What can you tell about the quality of information based on the provided
chromatogram? - answerThis chromatogram has a lot of "noise" and is likely an
unreliable sequence
Sequence information at the ends of the sequenced DNA: - answeris often unreliable
and should not be read
How much of the sequenced DNA is believed to be accurate? - answer500 -800 base
pairs in the middle of the sequence
