Bio 200 Prelab 4
The overall structure of the Gene Regulation part of the lab procedure this week is: -
answerAdd ONPG and heat, stop the reactions, measure yellow color on a
spectrophotometer, graph results, determine which culture was treated with which
sugar.
Why are some genes constitutively expressed while the expression of others is
regulated? - answerSome gene products are needed at all times, and other gene
products are only produced under some conditions to avoid waste and respond to
stimuli.
Which of these definitions fits which element of the DNA sequence?
This sequence element is used as the binding site of sigma and RNA polymerase,
which transcribes the DNA message into mRNA. All genes have one of these sites. -
answerPromoter
Which of these definitions fits which element of the DNA sequence?
Polymerase-blocking proteins, such as the LacI (repressor) protein, bind at these sites
to physically block movement of the transcribing RNA polymerase. - answerOperator
Which of these choices is an important DIFFERENCE between the Enzyme Lab
Procedure and this week's Gene Regulation Procedure? - answerThe Gene Regulation
Lab uses E. coli bacteria as a source of enzyme instead of the concentrated factory-
produced Lacteeze used in the Enzyme lab.
Why is transcriptional regulation is more common and/or efficient than translational
regulation? - answerTranslational regulation would require mRNA to be made for every
gene at all times, which is wasteful.
The overall structure of the Gene Regulation part of the lab procedure this week is: -
answerAdd ONPG and heat, stop the reactions, measure yellow color on a
spectrophotometer, graph results, determine which culture was treated with which
sugar.
Why are some genes constitutively expressed while the expression of others is
regulated? - answerSome gene products are needed at all times, and other gene
products are only produced under some conditions to avoid waste and respond to
stimuli.
Which of these definitions fits which element of the DNA sequence?
This sequence element is used as the binding site of sigma and RNA polymerase,
which transcribes the DNA message into mRNA. All genes have one of these sites. -
answerPromoter
Which of these definitions fits which element of the DNA sequence?
Polymerase-blocking proteins, such as the LacI (repressor) protein, bind at these sites
to physically block movement of the transcribing RNA polymerase. - answerOperator
Which of these choices is an important DIFFERENCE between the Enzyme Lab
Procedure and this week's Gene Regulation Procedure? - answerThe Gene Regulation
Lab uses E. coli bacteria as a source of enzyme instead of the concentrated factory-
produced Lacteeze used in the Enzyme lab.
Why is transcriptional regulation is more common and/or efficient than translational
regulation? - answerTranslational regulation would require mRNA to be made for every
gene at all times, which is wasteful.
