Bio 200 RQ 9-3
Where does PCR occur? - answerin a test tube in the lab (in vitro)
Which cellular process is PCR most similar to? - answerDNA replication
In order for PCR to work, what do you have to know about the DNA template you are
using? - answeryou have to know the sequences on either side of the region of DNA
you want to amplify
Which of the following are enzymes or substrates used in PCR? - answer-DNA
Polymerase
-dNTPs
If you start with just one copy of template DNA in PCR, how many copies will you have
after 10 cycles (if it works perfectly?) - answer2^10
Which of the following types of research have benefited from PCR technology? -
answer-Studying ancient DNA
-Analyzing DNA from crime scenes
-Determining whether a patient has an infection
Where does PCR occur? - answerin a test tube in the lab (in vitro)
Which cellular process is PCR most similar to? - answerDNA replication
In order for PCR to work, what do you have to know about the DNA template you are
using? - answeryou have to know the sequences on either side of the region of DNA
you want to amplify
Which of the following are enzymes or substrates used in PCR? - answer-DNA
Polymerase
-dNTPs
If you start with just one copy of template DNA in PCR, how many copies will you have
after 10 cycles (if it works perfectly?) - answer2^10
Which of the following types of research have benefited from PCR technology? -
answer-Studying ancient DNA
-Analyzing DNA from crime scenes
-Determining whether a patient has an infection
