BIOL 200 Pre-lab 6
Safety is a major issue in labs for the second half of Bio200.
In this lab in particular, there several different threats specific to this lab. Match these
correctly to the consequence if you are not careful. - answer Sharp urchin spines:
possible infection at two points
along the spine puncture wound
Hypodermic needle: extremely sharp and can inject poisonous substances directly into
your bloodstream
Razor blades: new metal is very sharp and difficult to cut with, so all cuts should be slow
and careful
Human saliva: the single best way to catch human disease is from direct contact with
bodily fluids
In this lab, we'll be using PCR to make many copies of a small region of your personal
DNA genome.
It is a very bad idea to touch anyone else's sample...but it is ok to touch your own
sample without gloves. Why?
(pick ALL true answers) - answerSaliva could contain human-specific viruses and
bacteria that we want to avoid.
Contamination of sample with your own DNA won't change your results.
This in-lab DNA purification is tricky. Unless all of the steps are done correctly, you are
unlikely to get a working result for PCR 2 lab next week. Take your time, and follow the
procedure carefully. If you are able to produce a successful result from the entire 2-
week procedure, you'll get 2 bonus points for the lab.
You'll need to repeat several steps until you can get a good-sized pellet. The procedure
says that the pellet should be roughly the same size as: - answera match head
How many times will we heat our DNA prep at 100 degrees Celsius?
(Note: this is hot enough to burn skin easily. Please be careful!) - answerOnce (for 5
minutes)
Centrifuges are a really simple and amazing piece of technology. Using simple
machinery, we can spin a tube of your cells extremely fast (faster than 14,000 times per
minute if necessary in our labs). However, if they are run incorrectly the centrifuge can
Safety is a major issue in labs for the second half of Bio200.
In this lab in particular, there several different threats specific to this lab. Match these
correctly to the consequence if you are not careful. - answer Sharp urchin spines:
possible infection at two points
along the spine puncture wound
Hypodermic needle: extremely sharp and can inject poisonous substances directly into
your bloodstream
Razor blades: new metal is very sharp and difficult to cut with, so all cuts should be slow
and careful
Human saliva: the single best way to catch human disease is from direct contact with
bodily fluids
In this lab, we'll be using PCR to make many copies of a small region of your personal
DNA genome.
It is a very bad idea to touch anyone else's sample...but it is ok to touch your own
sample without gloves. Why?
(pick ALL true answers) - answerSaliva could contain human-specific viruses and
bacteria that we want to avoid.
Contamination of sample with your own DNA won't change your results.
This in-lab DNA purification is tricky. Unless all of the steps are done correctly, you are
unlikely to get a working result for PCR 2 lab next week. Take your time, and follow the
procedure carefully. If you are able to produce a successful result from the entire 2-
week procedure, you'll get 2 bonus points for the lab.
You'll need to repeat several steps until you can get a good-sized pellet. The procedure
says that the pellet should be roughly the same size as: - answera match head
How many times will we heat our DNA prep at 100 degrees Celsius?
(Note: this is hot enough to burn skin easily. Please be careful!) - answerOnce (for 5
minutes)
Centrifuges are a really simple and amazing piece of technology. Using simple
machinery, we can spin a tube of your cells extremely fast (faster than 14,000 times per
minute if necessary in our labs). However, if they are run incorrectly the centrifuge can
