ASU BIO 340 Exam 3 Questions With
Correct Marking Scheme
/.Which activity of DNA polymerase is responsible for its ability to proofread
newly synthesized DNA?
exonuclease activity
/.How many mutations would you expect to see in the human genome (6 x 10^9) if
mismatches could not be repaired?
600,000
/.Proofreading removes approximately 99% of mismatches before DNA replication
moves on. After proofreading what is the mutation rate for DNA replication in
humans?
10^-6/base pair
/.Each of the five DNA strands below (A-E) are slowly heated. Which strand will
melt first?
A. 5'-ATATCGATTA-3'3'-TATAGCTAAT-5'
B. 5'-ACGGATGCAC-3'3'-TGCCTACGTG-5'
C. 5'-GCGCCCGGCG-3'3'-CGCGGGCCGC-5'
D. 5'-TTCGAGTGAA-3'3'-AAGCTCACTT-5
'E. 5'-CGCCGGATGC-3'3'-GCGGCCTACG-5'
/.The two oligonucleotides below are allowed to anneal and a set of sequencing
reactions are performed. What will the sequencing gel of the first 6 nucleotides
added to the DNA look like?
5'ATCCTGGACACTGTACCATCGGTACCAATCACAGGTCCTTACAGT 3'
5' CAGTGTC 3'
A
/.What is the sequence of the right-hand strand of the DNA shown below?
A. 5' ACTG 3'
B. 5' TGAC 3'
C. 3' CAGT 5'
, D. 3' TGAC 5'
E. 5' TGAC 3'
Which of the DNA strands has RNA on its left side?
A. A
B. B
C. C
D. D
E. C and D
/.Which of the DNA strands has its 3' end on the left side?
A. A
B. B
C. C
D. D
E. A and D
/.Which of the DNA strands has RNA on its right side?
A. A
B. B
C. C
D. D
E. C and D
/.Which of the following enzymatic activities (not necessarily called by the names
below)is not involved in DNA replication in eukaryotic cells?
A. Topoisomerase
B. DNA-dependent DNA polymerase
C. RNA-dependent DNA polymerase
D. RNA-dependent RNA polymerase
E. 3->5' DNA polymerase activity
/.What would the consequences be of having DNA polymerase without 5'->3'
exonuclease activity?
decreased polymerase fidelity
What is the correct order of enzyme action during DNA replication?
A. Ligation, priming, DNA polymerization, helicase/topoisomerase activity,
proofreading,5'-3' exonuclease activity/DNA polymerization.
B. Priming, helicase/topoisomerase activity, proofreading, DNA polymerization,
5'-3'exonuclease activity/DNA polymerization, ligation.
C. Helicase/topoisomerase activity, priming, 5'-3' exonuclease activity/DNA
Correct Marking Scheme
/.Which activity of DNA polymerase is responsible for its ability to proofread
newly synthesized DNA?
exonuclease activity
/.How many mutations would you expect to see in the human genome (6 x 10^9) if
mismatches could not be repaired?
600,000
/.Proofreading removes approximately 99% of mismatches before DNA replication
moves on. After proofreading what is the mutation rate for DNA replication in
humans?
10^-6/base pair
/.Each of the five DNA strands below (A-E) are slowly heated. Which strand will
melt first?
A. 5'-ATATCGATTA-3'3'-TATAGCTAAT-5'
B. 5'-ACGGATGCAC-3'3'-TGCCTACGTG-5'
C. 5'-GCGCCCGGCG-3'3'-CGCGGGCCGC-5'
D. 5'-TTCGAGTGAA-3'3'-AAGCTCACTT-5
'E. 5'-CGCCGGATGC-3'3'-GCGGCCTACG-5'
/.The two oligonucleotides below are allowed to anneal and a set of sequencing
reactions are performed. What will the sequencing gel of the first 6 nucleotides
added to the DNA look like?
5'ATCCTGGACACTGTACCATCGGTACCAATCACAGGTCCTTACAGT 3'
5' CAGTGTC 3'
A
/.What is the sequence of the right-hand strand of the DNA shown below?
A. 5' ACTG 3'
B. 5' TGAC 3'
C. 3' CAGT 5'
, D. 3' TGAC 5'
E. 5' TGAC 3'
Which of the DNA strands has RNA on its left side?
A. A
B. B
C. C
D. D
E. C and D
/.Which of the DNA strands has its 3' end on the left side?
A. A
B. B
C. C
D. D
E. A and D
/.Which of the DNA strands has RNA on its right side?
A. A
B. B
C. C
D. D
E. C and D
/.Which of the following enzymatic activities (not necessarily called by the names
below)is not involved in DNA replication in eukaryotic cells?
A. Topoisomerase
B. DNA-dependent DNA polymerase
C. RNA-dependent DNA polymerase
D. RNA-dependent RNA polymerase
E. 3->5' DNA polymerase activity
/.What would the consequences be of having DNA polymerase without 5'->3'
exonuclease activity?
decreased polymerase fidelity
What is the correct order of enzyme action during DNA replication?
A. Ligation, priming, DNA polymerization, helicase/topoisomerase activity,
proofreading,5'-3' exonuclease activity/DNA polymerization.
B. Priming, helicase/topoisomerase activity, proofreading, DNA polymerization,
5'-3'exonuclease activity/DNA polymerization, ligation.
C. Helicase/topoisomerase activity, priming, 5'-3' exonuclease activity/DNA
