BIO-357 Cell Bio Module 7 Quiz ASU
Based on the microscope image description, identify the cells in this culture. - answer
Stem Cells
Why are induced pluripotent stem cells (iPS) used instead of embryonic stem cells
(ES)? – answeri PS are better to avoid immunological reactions
The diagram above shows a eukaryote cell where the central dogma of molecular
biology is represented. This dogma shows the flow of genetic information. What are the
correct terms to fill boxes A, B, C, and D? - answer Transcription, pre-mRNA, mRNA,
ribosome
In eukaryotes which combination o molecules and DNA regions will increase the gene
expression rate? - answer Transcription factors and an enhancer region
Alright! Let's create some iPS from Amy's skin cells.
iPS can be developed by altering the gene expression of somatic cells (in this case skin
cells) using an adequate set of transcription factors.
Every cell in our body uses its own set of transcription factors to regulate gen
expression. We need to determine which set of transcription factors to use. - answerUse
unique transcription factors that can only be found in stem cells
Transduction is the process whereby foreign DNA is introduced into another cell via a
viral vector. In this lab, four transcription factor genes were transduced into fibroblast
cells. Which virus did you use today? - answer3
Look at the screen next to the microscope to examine the morphology of the cell lines.
Remember to examine both plates from the incubator.
Based on your examination, which cell line exhibits stem-like morphology? - answerThe
one that was transduced with the unique transcription factors
The gene expression of NANOG is upregulated in iPS (Induced Pluripotent Stem) cells
but silenced in differentiated cells. What does this suggest about NANOG? -
answerSustains stem cell's self-renewal ability
Why do we need to analyze the samples resulting from the RT-PCR from embryonic
stem cells and fibroblast cells as well? - answerBecause those samples are the
experimental controls
Examine the RT-PCR result that shows gene expression of NANOG in the cells. Which
cells express NANOG? - answerCells that have been transduced with unique
transcription factors
Based on the microscope image description, identify the cells in this culture. - answer
Stem Cells
Why are induced pluripotent stem cells (iPS) used instead of embryonic stem cells
(ES)? – answeri PS are better to avoid immunological reactions
The diagram above shows a eukaryote cell where the central dogma of molecular
biology is represented. This dogma shows the flow of genetic information. What are the
correct terms to fill boxes A, B, C, and D? - answer Transcription, pre-mRNA, mRNA,
ribosome
In eukaryotes which combination o molecules and DNA regions will increase the gene
expression rate? - answer Transcription factors and an enhancer region
Alright! Let's create some iPS from Amy's skin cells.
iPS can be developed by altering the gene expression of somatic cells (in this case skin
cells) using an adequate set of transcription factors.
Every cell in our body uses its own set of transcription factors to regulate gen
expression. We need to determine which set of transcription factors to use. - answerUse
unique transcription factors that can only be found in stem cells
Transduction is the process whereby foreign DNA is introduced into another cell via a
viral vector. In this lab, four transcription factor genes were transduced into fibroblast
cells. Which virus did you use today? - answer3
Look at the screen next to the microscope to examine the morphology of the cell lines.
Remember to examine both plates from the incubator.
Based on your examination, which cell line exhibits stem-like morphology? - answerThe
one that was transduced with the unique transcription factors
The gene expression of NANOG is upregulated in iPS (Induced Pluripotent Stem) cells
but silenced in differentiated cells. What does this suggest about NANOG? -
answerSustains stem cell's self-renewal ability
Why do we need to analyze the samples resulting from the RT-PCR from embryonic
stem cells and fibroblast cells as well? - answerBecause those samples are the
experimental controls
Examine the RT-PCR result that shows gene expression of NANOG in the cells. Which
cells express NANOG? - answerCells that have been transduced with unique
transcription factors
