Cell BIO 357 ASU Quiz 3
What does it mean to have parfocal objectives? - answer The sample remains in focus
when the objectives are changed
What factor improved by adding immersion oil when using the 100X objective? - answer
Resolution
Can the scientist use the samples they have already stained with the Mallory stain to
study goblet cell numbers and morphology? - answer Yes, the outline of the cell, the
nucleus and mucus each have a different color.
What can be an advantage of fluorescence microscopy over light microscopy? -
answerStudy cell processes in living cells.
If you want to image multiple structures using fluorescence microscopy, which of these
considerations should you take into account when choosing fluorophores? - answerThe
emission wavelengths of the fluorophores should not overlap.
Remember that you can see the cells outlined in yellow with the nucleus in cyan and
that the virus is labeled in magenta. Does the virus infect all the cells in the chicken
intestine to the same extent? - answerNo, the viral protein is concentrated in the lumen
and a few cells.
What is the purpose of an emission filter in the fluorescence microscope? - answerOnly
the emitted wavelength reaches the eyepiece and camera
How can plant diseases reduce the yield of crops? - answerBy reducing the
photosynthetic areas on leaves.
Confocal microscopy increases both the optical resolution and contrast of an image
compared to widefield microscopy. What's your guess on how this is achieved? -
answerThere is a pinhole to filter out unfocused light.
Which of the following is NOT an advantage of using confocal microscopy over widefield
microscopy? - answerIt's cheaper than conventional light microscopy methods.
Why is a photo multiplier tube (PMT) needed as the detector? - answerTo increase the
fluorescent signal
What are fluorophores? - answerThey are chemical compounds that re-emit light of a
longer wavelength after exposure
What does it mean to have parfocal objectives? - answer The sample remains in focus
when the objectives are changed
What factor improved by adding immersion oil when using the 100X objective? - answer
Resolution
Can the scientist use the samples they have already stained with the Mallory stain to
study goblet cell numbers and morphology? - answer Yes, the outline of the cell, the
nucleus and mucus each have a different color.
What can be an advantage of fluorescence microscopy over light microscopy? -
answerStudy cell processes in living cells.
If you want to image multiple structures using fluorescence microscopy, which of these
considerations should you take into account when choosing fluorophores? - answerThe
emission wavelengths of the fluorophores should not overlap.
Remember that you can see the cells outlined in yellow with the nucleus in cyan and
that the virus is labeled in magenta. Does the virus infect all the cells in the chicken
intestine to the same extent? - answerNo, the viral protein is concentrated in the lumen
and a few cells.
What is the purpose of an emission filter in the fluorescence microscope? - answerOnly
the emitted wavelength reaches the eyepiece and camera
How can plant diseases reduce the yield of crops? - answerBy reducing the
photosynthetic areas on leaves.
Confocal microscopy increases both the optical resolution and contrast of an image
compared to widefield microscopy. What's your guess on how this is achieved? -
answerThere is a pinhole to filter out unfocused light.
Which of the following is NOT an advantage of using confocal microscopy over widefield
microscopy? - answerIt's cheaper than conventional light microscopy methods.
Why is a photo multiplier tube (PMT) needed as the detector? - answerTo increase the
fluorescent signal
What are fluorophores? - answerThey are chemical compounds that re-emit light of a
longer wavelength after exposure
