Clinical Chemistry Review - Proteins: Questions
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Terms in this set (50)
What is the most Protein. The reference rage is 6-8g/dL. Most other
concentrated solute of chemistry analytes are measured in mg/dL.
the blood?
Which element is found Nitrogen. The protein molecule is 16% nitrogen.
in proteins but not in
carbohydrates or lipids.
Where are most proteins In the liver.
synthesized?
What is the waste Urea. It is excreted in the urine.
product of protein
catabolism?
Heat, pH, enzymes,, and UV light all can disrupt the
What causes
molecular structure of protein, resulting in loss of
denaturation of protein?
function.
Why should repeated Repeated freezing and thawing may cause proteins
freezing and thawing of a to deteriorate.
specimen be avoided?
What is the most The biuret method. Cuprous ions react with peptide
common method for bonds at an alkaline pH to produce a colored
measuring serum total complex
protein?
Why is the biuret method It is not sensitive enough for the small amount of
of protein analysis not protein in the urine. Trichloracetic acid can be used
suitable for urine or CSF to precipitate protein in urine and CSF.
protein?
, How would hemolysis Hemoglobin in the serum would increase the
affect the serum total protein level.
protein level?
What does low total Edema. Water leaves the blood vessels and goes
protein cause in the into the tissues.
patient?
It maintains the osmotic pressure and the
What is the main distribution of water in the body. When albumin is
physiological function of low, edema results. Albumin also has an important
albumin? role in binding and transporting various substances
in the blood such as bilirubin and some drugs.
What are the most Bromcresol green (BCG) and bromcresol purple
common dyes for the (BCP).
direct analysis of
albumin?
What happens to total They decrease due to loss through the urine.
protein and albumin
levels in the nephrotic
syndrome?
How is the concentration By subtracting albumin from total protein
of globulins determined
in a chemistry profile?
1-1.8 a reversed A:G ratio is seen with monoclonal
What is the normal A:G
gammopathies and other increases in globulin
ratio?
fraction.
0.84 - Globulins are determined by subtracting the
Calculate the A:G ratio
albumin from the total protein (9.2-4.2 = 5.0) A:G
for patient whose total
ratio is determined by dividing albumin by globulin
protein is 9.2 g/dL and
(4.2/5.0 = 0.84) The normal ratio is 1.0-1.8 This patient
whose albumin is 4.2
has a reversed ratio, indicating increased globulins,
g/dL.
possibly due to gammopathy.
The property of proteins to assume a positive and
negative or neutral charge, depending on the pH of
What is amphoterism?
the medium. With serum protein electrophoresis at
pH 8.6, proteins carry a negative charge.
Complete With A+ Solutions
Save
Terms in this set (50)
What is the most Protein. The reference rage is 6-8g/dL. Most other
concentrated solute of chemistry analytes are measured in mg/dL.
the blood?
Which element is found Nitrogen. The protein molecule is 16% nitrogen.
in proteins but not in
carbohydrates or lipids.
Where are most proteins In the liver.
synthesized?
What is the waste Urea. It is excreted in the urine.
product of protein
catabolism?
Heat, pH, enzymes,, and UV light all can disrupt the
What causes
molecular structure of protein, resulting in loss of
denaturation of protein?
function.
Why should repeated Repeated freezing and thawing may cause proteins
freezing and thawing of a to deteriorate.
specimen be avoided?
What is the most The biuret method. Cuprous ions react with peptide
common method for bonds at an alkaline pH to produce a colored
measuring serum total complex
protein?
Why is the biuret method It is not sensitive enough for the small amount of
of protein analysis not protein in the urine. Trichloracetic acid can be used
suitable for urine or CSF to precipitate protein in urine and CSF.
protein?
, How would hemolysis Hemoglobin in the serum would increase the
affect the serum total protein level.
protein level?
What does low total Edema. Water leaves the blood vessels and goes
protein cause in the into the tissues.
patient?
It maintains the osmotic pressure and the
What is the main distribution of water in the body. When albumin is
physiological function of low, edema results. Albumin also has an important
albumin? role in binding and transporting various substances
in the blood such as bilirubin and some drugs.
What are the most Bromcresol green (BCG) and bromcresol purple
common dyes for the (BCP).
direct analysis of
albumin?
What happens to total They decrease due to loss through the urine.
protein and albumin
levels in the nephrotic
syndrome?
How is the concentration By subtracting albumin from total protein
of globulins determined
in a chemistry profile?
1-1.8 a reversed A:G ratio is seen with monoclonal
What is the normal A:G
gammopathies and other increases in globulin
ratio?
fraction.
0.84 - Globulins are determined by subtracting the
Calculate the A:G ratio
albumin from the total protein (9.2-4.2 = 5.0) A:G
for patient whose total
ratio is determined by dividing albumin by globulin
protein is 9.2 g/dL and
(4.2/5.0 = 0.84) The normal ratio is 1.0-1.8 This patient
whose albumin is 4.2
has a reversed ratio, indicating increased globulins,
g/dL.
possibly due to gammopathy.
The property of proteins to assume a positive and
negative or neutral charge, depending on the pH of
What is amphoterism?
the medium. With serum protein electrophoresis at
pH 8.6, proteins carry a negative charge.
