Chemistry ASCP BOC Review – Questions With
Appropriate Solutions
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Practice questions for this set
Learn 1/6 Study with Learn
Prehepatic and posthepatic
Choose an answer
Chemical rxn produces colored
substance that absorbs light of a
1 Heparin on ice used for? 2
specific wavelength; one of most
common methods
ACTH, cortisol, catecholamines
3 Unconjugated bilirubin is increased in... 4
increased with?
Don't know?
Terms in this set (164)
Heparin on ice used for? Ammonia and blood gasses
Analyte affected because Cortisol, ACTH, iron, GH, PTH, TSH
of diurnal variation
Day to day variation is ALT, bili, CK, steroid hormones, triglycerides
normal for which analyte?
,Alcohol's affect on glucose, -decrease glucose
triglycerides, GGT -increase triglycerides and GGT
ACTH, cortisol, Stress
catecholamines increased
with?
IV fluid contamination glucose, Na, K, Cl
increases which?
Sodium fluoride (gray top) Glucose bc it slows glycolysis (NOT for BUN)
used for?
EDTA is NOT ok for which Na, K, or Ca
analytes?
What analytes increase with K, Mg, PO4, LD, AST, iron, ammonia
hemolysis?
Which analytes are higher total protein, LD, Ca
in plasma than serum?
Higher in RBCs than plasma K+, phosphate, Mg2+
Chilling required for which? Lactic acid, ammonia, blood gasses
Chemical rxn produces spectrophotometry
colored substance that
absorbs light of a specific
wavelength; one of most
common methods
measures light absorbed atomic absorption
by ground state atoms;
measures trace metals;
hollow cathode lamp;
sensitive method
atoms absorb light of Fluorometry
specific wavelength and
emit light of longer
wavelength at lower
energy; mercury or xenon
light lamp; detector at 90
degrees; measures drugs
and horomones
, chemical rxn that produces Chemilumininescence
light (usually involved
oxidation of luminol,
acridium, esters or
dioxetanes); does not
require excitation radiation
or monochromators;
extremely sensitive; used
for immunoassays
Measures reduction in light turbidimetry
transmission by particles in
suspension; used to
measure proteins in urine
and CSF
Similar to turbidity, but light Nephelometry
is measured at angle from
light source. Measures agn-
aby rxns.
visible spectrum of light 400-700 nm
Screening used for drugs TLC
of abuse in urine; identified
by Rf value (distance
traveled by
compound/distance
traveled by solvent)
Separation of thermolabile HPLC
compounds; concentration
determined by peak height
ratio; MS can be used for
definitive ID
Separation of volatile GC
compounds; therapeutic
and toxic drugs; ID'd by
retention time; MS used for
definitive ID
Potential difference Ion-selective electrodes
between 2 electrodes
directly related to
concentration of analyte
Appropriate Solutions
Save
Practice questions for this set
Learn 1/6 Study with Learn
Prehepatic and posthepatic
Choose an answer
Chemical rxn produces colored
substance that absorbs light of a
1 Heparin on ice used for? 2
specific wavelength; one of most
common methods
ACTH, cortisol, catecholamines
3 Unconjugated bilirubin is increased in... 4
increased with?
Don't know?
Terms in this set (164)
Heparin on ice used for? Ammonia and blood gasses
Analyte affected because Cortisol, ACTH, iron, GH, PTH, TSH
of diurnal variation
Day to day variation is ALT, bili, CK, steroid hormones, triglycerides
normal for which analyte?
,Alcohol's affect on glucose, -decrease glucose
triglycerides, GGT -increase triglycerides and GGT
ACTH, cortisol, Stress
catecholamines increased
with?
IV fluid contamination glucose, Na, K, Cl
increases which?
Sodium fluoride (gray top) Glucose bc it slows glycolysis (NOT for BUN)
used for?
EDTA is NOT ok for which Na, K, or Ca
analytes?
What analytes increase with K, Mg, PO4, LD, AST, iron, ammonia
hemolysis?
Which analytes are higher total protein, LD, Ca
in plasma than serum?
Higher in RBCs than plasma K+, phosphate, Mg2+
Chilling required for which? Lactic acid, ammonia, blood gasses
Chemical rxn produces spectrophotometry
colored substance that
absorbs light of a specific
wavelength; one of most
common methods
measures light absorbed atomic absorption
by ground state atoms;
measures trace metals;
hollow cathode lamp;
sensitive method
atoms absorb light of Fluorometry
specific wavelength and
emit light of longer
wavelength at lower
energy; mercury or xenon
light lamp; detector at 90
degrees; measures drugs
and horomones
, chemical rxn that produces Chemilumininescence
light (usually involved
oxidation of luminol,
acridium, esters or
dioxetanes); does not
require excitation radiation
or monochromators;
extremely sensitive; used
for immunoassays
Measures reduction in light turbidimetry
transmission by particles in
suspension; used to
measure proteins in urine
and CSF
Similar to turbidity, but light Nephelometry
is measured at angle from
light source. Measures agn-
aby rxns.
visible spectrum of light 400-700 nm
Screening used for drugs TLC
of abuse in urine; identified
by Rf value (distance
traveled by
compound/distance
traveled by solvent)
Separation of thermolabile HPLC
compounds; concentration
determined by peak height
ratio; MS can be used for
definitive ID
Separation of volatile GC
compounds; therapeutic
and toxic drugs; ID'd by
retention time; MS used for
definitive ID
Potential difference Ion-selective electrodes
between 2 electrodes
directly related to
concentration of analyte
