ASCP BOC Prep - Clinical Chemistry: Questions &
Accurate Solutions
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Terms in this set (345)
Characterized as a spectrum from short wavelength to
Radiant Energy long wavelength: cosmic, gamma rays, X-rays, UV,
visible, infrared, microwaves, radio waves
The distance traveled between one complete wave
Wavelength cycle (distance between two successive crests)
measured in nanometers (nm)
the greater the energy contained in the light and the
The shorter the wavelength
greater the number of photons
UV light has Short
very____________________
wavelengths
Infared light has very Long
_________________________
wavelengths.
When all visible White light
wavelengths of light are
combined________________
___________________ results.
Wavelength of light transmitted (not absorbed) by an
Visible Color
object
Photons particles of light
When energy is absorbed Electron, Unstable
as a photon,
an______________________ is moved
to a higher energy level
where it is
__________________________.
,Three ways that atom 1. Electron is moved to a higher energy level
becomes excited when it 2. The mode of the covalent bond vibration is changed
absorbs a photon: 3. rotation around its covalent bonds is changed
An excited electron is Stable, Ground State
not_____________________ and will
return to ________________________.
An electron will Emit, Light
___________________ energy in the
form of ____________________
(radiant energy) of a
characteristic wavelength
Absorption or emission of Line Spectrum
energy forms a
___________________
_____________________ that is
characteristic of a molecule
and can help identify a
molecule.
_________________________________ Spectrophotometer
measures light transmitted
by an analyte in solution.
Analyte may Absorb, Transmit, or Reflect
_____________________,
____________________, and
____________________ light to
varying degrees, but always
of a characteristic nature
for the analyte.
The _____________________ Light Source or Exciter Lamp
_________________________ of a
spectrophotometer
produces an intense,
reproducible, constant
beam of light.
Types of incandescent Tungsten (most common) - Visible and infared
lamps in a Deuterium (UV)
spectrophotometer
,What must be done to the Calibration
spectrophotometer if a
lamp is changed before
analyzing patient samples
Optical device that transmits a mechanically selectable
Monochromater narrow band of wavelengths of light in a
spectrophotometer.
Type of monochromater in Glass filters and interference filters
a photometer
Type of monochromater diffraction grating and prisim
used in a
spectrophotometer
The ______________________ or Bandpass or Spectral bandwidth
_________________________
_____________________________ is the
range of wavelengths in
nanometers that is
transmitted by the
monochromator.
Principal of Photo detector
______________________________: a
detector converts the
electromagnetic radiation
(light energy) transmitted
by a solution into an
electrical signal. The more
light transmitted the more
energy, and greater
electrical signal that is
measured.
_________________________________: Readout device - spectrophotometer
electrical energy from a
detector is displayed in
some type of digital display
or readout system. The
readout system may be a
chart recorder or a
computer print out.
, Principal of _______________ Atomic Absorption Spectrophotometery
______________________: ground-
state atoms absorb light at
defined wavelengths.
Wavelengths at which an Line spectrum
atom absorbs light; each
metal exhibits a specific
one.
Atomic absorption atomized
spectrophotometry:
Sample is
_____________________________ in a
flame where the atoms of
the metal to be quantified
are maintained at ground
state.
Atomic absorption Hollow-cathode lamp (HCL)
spectrophotometry: After
the sample is atomized in
flame the beam of light
from a ______________________
_______________________
___________________________ is
passed through a chopper
to the flame.
Atomic absorption Ground state atoms
spectrophotometry: After
the light from the HCL is
passed through the
chopper; the __________________
_______________________
___________________ in the flame
absorb the same
wavelengths of light from
the HCL as the atoms emit
when excited.
Accurate Solutions
Save
Terms in this set (345)
Characterized as a spectrum from short wavelength to
Radiant Energy long wavelength: cosmic, gamma rays, X-rays, UV,
visible, infrared, microwaves, radio waves
The distance traveled between one complete wave
Wavelength cycle (distance between two successive crests)
measured in nanometers (nm)
the greater the energy contained in the light and the
The shorter the wavelength
greater the number of photons
UV light has Short
very____________________
wavelengths
Infared light has very Long
_________________________
wavelengths.
When all visible White light
wavelengths of light are
combined________________
___________________ results.
Wavelength of light transmitted (not absorbed) by an
Visible Color
object
Photons particles of light
When energy is absorbed Electron, Unstable
as a photon,
an______________________ is moved
to a higher energy level
where it is
__________________________.
,Three ways that atom 1. Electron is moved to a higher energy level
becomes excited when it 2. The mode of the covalent bond vibration is changed
absorbs a photon: 3. rotation around its covalent bonds is changed
An excited electron is Stable, Ground State
not_____________________ and will
return to ________________________.
An electron will Emit, Light
___________________ energy in the
form of ____________________
(radiant energy) of a
characteristic wavelength
Absorption or emission of Line Spectrum
energy forms a
___________________
_____________________ that is
characteristic of a molecule
and can help identify a
molecule.
_________________________________ Spectrophotometer
measures light transmitted
by an analyte in solution.
Analyte may Absorb, Transmit, or Reflect
_____________________,
____________________, and
____________________ light to
varying degrees, but always
of a characteristic nature
for the analyte.
The _____________________ Light Source or Exciter Lamp
_________________________ of a
spectrophotometer
produces an intense,
reproducible, constant
beam of light.
Types of incandescent Tungsten (most common) - Visible and infared
lamps in a Deuterium (UV)
spectrophotometer
,What must be done to the Calibration
spectrophotometer if a
lamp is changed before
analyzing patient samples
Optical device that transmits a mechanically selectable
Monochromater narrow band of wavelengths of light in a
spectrophotometer.
Type of monochromater in Glass filters and interference filters
a photometer
Type of monochromater diffraction grating and prisim
used in a
spectrophotometer
The ______________________ or Bandpass or Spectral bandwidth
_________________________
_____________________________ is the
range of wavelengths in
nanometers that is
transmitted by the
monochromator.
Principal of Photo detector
______________________________: a
detector converts the
electromagnetic radiation
(light energy) transmitted
by a solution into an
electrical signal. The more
light transmitted the more
energy, and greater
electrical signal that is
measured.
_________________________________: Readout device - spectrophotometer
electrical energy from a
detector is displayed in
some type of digital display
or readout system. The
readout system may be a
chart recorder or a
computer print out.
, Principal of _______________ Atomic Absorption Spectrophotometery
______________________: ground-
state atoms absorb light at
defined wavelengths.
Wavelengths at which an Line spectrum
atom absorbs light; each
metal exhibits a specific
one.
Atomic absorption atomized
spectrophotometry:
Sample is
_____________________________ in a
flame where the atoms of
the metal to be quantified
are maintained at ground
state.
Atomic absorption Hollow-cathode lamp (HCL)
spectrophotometry: After
the sample is atomized in
flame the beam of light
from a ______________________
_______________________
___________________________ is
passed through a chopper
to the flame.
Atomic absorption Ground state atoms
spectrophotometry: After
the light from the HCL is
passed through the
chopper; the __________________
_______________________
___________________ in the flame
absorb the same
wavelengths of light from
the HCL as the atoms emit
when excited.
