1st Bimonthly- MICRO PARA PRACTICAL
⭐Staphyloxanthin
Yellow pigment:
Ferments: Carbohydrates
Media: Mannitol Salt Agar
Staphylococcus aureus Streptococcus hemolyticus Beta Hemolytics - Group A Streptococcus (S. Pyogenes),
Gram +, Cocci,Clusters Group B Streptococcus (S. Agalactiae)
NM: Upper Respiratory Tract, Skin Gram +, Cocci, Chains
DOC: Vancomycin (if MRSA) NM: Pharyngeal/Pharynx Alpha Hemolytics - Viridans streptococci
If Beta Lactamase resistant penicillin: Dicloxacillin, DOC: Penicillin V/ Penicillin G
Oxacillin, Cloxacillin Ass. dss: Pharyngitis, Impetigo
Ass. dss: Toxic Shock Syndrome, Cellulitis
Diplococcus pneumoniae Gaffkya tetragena Sarcina lutea
Aka: Streptococcus pneumoniae
Gram +, cocci,diplococci,lancet-shaped Aka: Micrococcus tetragenus Gram +, cocci, cuboidal form
Ass. dss: Pneumococcal pneumonia and ear infection Gram +, Cocci, Tetrads NM: Intestine, Stomach
Triad of severe complications: meningitis, endocarditis, Ass. dss: Soft tissue infection Ass. dss: Gastroenteritis, present with vomiting
and septic arthritis TOC: Tetracycline
TOC: High dose penicillin( except for meningitis)
Vancomycin
Diagnosis:Alpha-hemolytic (+) Quellung test
⭐
Give 2 structural component: Capsule and
Pneumolysin
, Bacillus subtilis Mycobacterium tuberculosis Treatment:
Neither gram (+) nor gram (-),bacillus,diplobacilli H- Isoniazid, R- Rifampin, Z- Pyrazinamide, E- Ethambutol
Gram +, bacillus, chain Appearance: Ghost cells To add:
Normally: Non pathogenic Growth Medium: Lowenstein- Jensen (LJ) Medium - M. tuberculosis contains Metachromatic
Immunocompromised patients: Opportunistic Stains brighter when using: Ziehl-Neelsen / Kinyoun Granules, which are made up of metaphosphate
Source of Bacitracin: Used in fungicide stain. accumulation inside the cell.
Prevalent in soil, water, air, and on vegetation Aerobic, slow-growing - “Metachromatic” - stain red w/ methylene blue.
⭐Mycolic
DOC: Vancomycin Frequent source of infection comes from humans.
2 different forms : Structure that is notable in this organism?
Snapping Acid in Peptidoglycan Cell Wall
Slipping
Clostridium tetani Escherichia coli Neisseria gonorrhoeae
Gram -, coccobacilli, elongated/oval-shaped
Gram +, tennis racket appearance NM: Lower GI Tract Gram -, diplococci, kidney/coffee-bean shaped in pairs
Terminal spores: Location inside cell (Terminal) Pathogenesis & Clinical Manifestations: Obtained from: Cervical purulent secretion
Toxin: Causes tetanus Urinary Tract Infections Infection: STI
Management: prevention is all important.
Active immunization
Aggressive wound care
E.coli associated diarrhea
Treatment: Sulfonamides, ampicillin,cephalosporins,
fluoroquinolones
⭐ Risk Factor: Sexual contact
Sugar fermentation reaction w/ glucose: Positive
Eosin Methylene Blue (EMB) agar: Greenish metallic
sheen
Pink: MacConki Agar
, Corynebacterium diphtheriae Rhizopus Stolonifer Mucor sp.
Gram +, club shaped, “chinese character appearance” Identify: Identify:
Cause of agent: Respiratory or cutaneous diphtheria 1. Sporangium, Sporangium
Treatment: Penicillin (Antimicrobial Drugs) 2.Sporangiospores, Sporangiophore
⭐Specific structure: Metachromatic/Volutin granules 3.Columella,
4. Stolon,
5. Rhizoids
Sporangiospores
Major Clinical Form
Rhinocerebral mucormycosis
Characterized by:
-presence of stolon Treatment
Specimen obtained from:
-pigmented rhizoid Amp B
Environment
Aggressive surgical
debridement
Aspergillus sp. Penicillium sp. Candida albicans
Structures: Budding yeast, presence of pseudohyphae
Identify: 1. Conidiophore 2. Vesicle 3. Metula 4. Phialides Identify: Reproduction: Asexual(Fission)
5. Conidia 1. Conidiophore Clinical Importance: Most common yeast pathogen
Treatment(Oral candidiasis): Amphotericin B (AMB)
Ass. dss: Aspergillosis,/Aspergilloma 2. Metula (Vulvovaginitis): Topical azole
Treatment: Amphotericin B (Voriconazole/ Itraconazole) 3. Phialides S/E: fever and chills
4. Conidia Meperidine: Shortens the episode
⭐Staphyloxanthin
Yellow pigment:
Ferments: Carbohydrates
Media: Mannitol Salt Agar
Staphylococcus aureus Streptococcus hemolyticus Beta Hemolytics - Group A Streptococcus (S. Pyogenes),
Gram +, Cocci,Clusters Group B Streptococcus (S. Agalactiae)
NM: Upper Respiratory Tract, Skin Gram +, Cocci, Chains
DOC: Vancomycin (if MRSA) NM: Pharyngeal/Pharynx Alpha Hemolytics - Viridans streptococci
If Beta Lactamase resistant penicillin: Dicloxacillin, DOC: Penicillin V/ Penicillin G
Oxacillin, Cloxacillin Ass. dss: Pharyngitis, Impetigo
Ass. dss: Toxic Shock Syndrome, Cellulitis
Diplococcus pneumoniae Gaffkya tetragena Sarcina lutea
Aka: Streptococcus pneumoniae
Gram +, cocci,diplococci,lancet-shaped Aka: Micrococcus tetragenus Gram +, cocci, cuboidal form
Ass. dss: Pneumococcal pneumonia and ear infection Gram +, Cocci, Tetrads NM: Intestine, Stomach
Triad of severe complications: meningitis, endocarditis, Ass. dss: Soft tissue infection Ass. dss: Gastroenteritis, present with vomiting
and septic arthritis TOC: Tetracycline
TOC: High dose penicillin( except for meningitis)
Vancomycin
Diagnosis:Alpha-hemolytic (+) Quellung test
⭐
Give 2 structural component: Capsule and
Pneumolysin
, Bacillus subtilis Mycobacterium tuberculosis Treatment:
Neither gram (+) nor gram (-),bacillus,diplobacilli H- Isoniazid, R- Rifampin, Z- Pyrazinamide, E- Ethambutol
Gram +, bacillus, chain Appearance: Ghost cells To add:
Normally: Non pathogenic Growth Medium: Lowenstein- Jensen (LJ) Medium - M. tuberculosis contains Metachromatic
Immunocompromised patients: Opportunistic Stains brighter when using: Ziehl-Neelsen / Kinyoun Granules, which are made up of metaphosphate
Source of Bacitracin: Used in fungicide stain. accumulation inside the cell.
Prevalent in soil, water, air, and on vegetation Aerobic, slow-growing - “Metachromatic” - stain red w/ methylene blue.
⭐Mycolic
DOC: Vancomycin Frequent source of infection comes from humans.
2 different forms : Structure that is notable in this organism?
Snapping Acid in Peptidoglycan Cell Wall
Slipping
Clostridium tetani Escherichia coli Neisseria gonorrhoeae
Gram -, coccobacilli, elongated/oval-shaped
Gram +, tennis racket appearance NM: Lower GI Tract Gram -, diplococci, kidney/coffee-bean shaped in pairs
Terminal spores: Location inside cell (Terminal) Pathogenesis & Clinical Manifestations: Obtained from: Cervical purulent secretion
Toxin: Causes tetanus Urinary Tract Infections Infection: STI
Management: prevention is all important.
Active immunization
Aggressive wound care
E.coli associated diarrhea
Treatment: Sulfonamides, ampicillin,cephalosporins,
fluoroquinolones
⭐ Risk Factor: Sexual contact
Sugar fermentation reaction w/ glucose: Positive
Eosin Methylene Blue (EMB) agar: Greenish metallic
sheen
Pink: MacConki Agar
, Corynebacterium diphtheriae Rhizopus Stolonifer Mucor sp.
Gram +, club shaped, “chinese character appearance” Identify: Identify:
Cause of agent: Respiratory or cutaneous diphtheria 1. Sporangium, Sporangium
Treatment: Penicillin (Antimicrobial Drugs) 2.Sporangiospores, Sporangiophore
⭐Specific structure: Metachromatic/Volutin granules 3.Columella,
4. Stolon,
5. Rhizoids
Sporangiospores
Major Clinical Form
Rhinocerebral mucormycosis
Characterized by:
-presence of stolon Treatment
Specimen obtained from:
-pigmented rhizoid Amp B
Environment
Aggressive surgical
debridement
Aspergillus sp. Penicillium sp. Candida albicans
Structures: Budding yeast, presence of pseudohyphae
Identify: 1. Conidiophore 2. Vesicle 3. Metula 4. Phialides Identify: Reproduction: Asexual(Fission)
5. Conidia 1. Conidiophore Clinical Importance: Most common yeast pathogen
Treatment(Oral candidiasis): Amphotericin B (AMB)
Ass. dss: Aspergillosis,/Aspergilloma 2. Metula (Vulvovaginitis): Topical azole
Treatment: Amphotericin B (Voriconazole/ Itraconazole) 3. Phialides S/E: fever and chills
4. Conidia Meperidine: Shortens the episode
