Chromatography Exam Questions and Answers 100% Pass

Chromatography Exam Questions and
Answers 100% Pass


Use of chromatography and different types -

[ANSWER]: - Chromatography is useful because it enables the

separation, isolation, purification, and identification of components of

extremely complex mixtures

- There are different chromatography techniques:

- Paper chromatography

- Column chromatography

- Thin layer chromatography

- The results of chromatographic analysis are called chromatograms

- The number of spots or peaks correspond to the number of

components in the sample

- If only one spot appears, then the substance being tested is pure

, - Identification can be carried out in planar chromatography by

matching colour or distance of an unknown sample with reference

compounds




QUESTION: Paper chromatography -

[ANSWER]: - If a mixture is dropped onto a piece of blotting paper,

the components spread out

- The degree of spreading depends on how well the components stick to

the solvent molecules present in the piece of paper

- Th components which have the least solubility in the solvent are those

which tend to stick to the paper and so do not spread out as much as

those which are. more soluble in the solvent (so they tend not to stick

and therefore spread further up the paper)




QUESTION: Adsorption chromatography -

[ANSWER]: - Adsorption chromatography involves the use of a

stationary phase and a mobile phase to separate the components of a

mixture