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BIO 340 Final Exam/ 491 Questions & 100% Detailed
Answers/ A+ Score Solution||
An individual is heterozygous at two, unlinked autosomal
local (A and B) if its genotype is Aa Bb, what gametes
does it produce? - ANSWER-AB, Ab, aB, ab
An individual is heterozygous at two, unlinked autosomal
local (A and B). What are its gamete ratios? - ANSWER-
1:1:1:1
An individual is heterozygous at two, LINKED autosomal
loci (E and F). What is its chromosomal arrangement? -
ANSWER-=E===F= and =e===f=
=E===f= and =e===F=
,2|Page
A double heterozygous has phase Ef/eF. What are its
parental chromosomes/gametes? - ANSWER-Ef and eF
Which protein is responsible for the initial denaturing of
otic in E.coli? - ANSWER-DnaA
-the DnaA protein initially binds to the 9-mer repeats in
otic. The bound couple of DnaA proteins bend the DNA
and stimulates denaturation of the AT-rich 13-mer region.
In a nucleotide, to which carbon are the phosphates and
base attached? - ANSWER-5' phosphate- 1' base
,3|Page
- in nucleotides, the phosphate group is attached to the 5'
carbon and the base is attached to the 1' carbon
reaction ingredients - ANSWER-1. DNA template with
region to be copied (amplified)
2. dkeoxynuceotide triphosphate= dNTPs (dCTP, dTTP,
dATP, dGTP)
3. polymerase (usually Taq)
4. aqueous buffer (including MgCl₂)
5.DNA primers
6. fluorescently labeled dideoxynucleotide triphosphate=
ddNTPs
, 4|Page
Which of the following is the correct sequence of steps in
a typical PCR sequence - ANSWER-denaturation, primer
annealing, primer extension
Denaturation - ANSWER-reaction is heated to approx. 95
degrees C to separate DNA
Primer annealing - ANSWER-reaction is heating cooled to
45-68 degrees C to allow primers to bind to template
strand
Primer extension - ANSWER-reaction is heated to approx.
72 degrees C for primer extension (synthesis of target
DNA)
BIO 340 Final Exam/ 491 Questions & 100% Detailed
Answers/ A+ Score Solution||
An individual is heterozygous at two, unlinked autosomal
local (A and B) if its genotype is Aa Bb, what gametes
does it produce? - ANSWER-AB, Ab, aB, ab
An individual is heterozygous at two, unlinked autosomal
local (A and B). What are its gamete ratios? - ANSWER-
1:1:1:1
An individual is heterozygous at two, LINKED autosomal
loci (E and F). What is its chromosomal arrangement? -
ANSWER-=E===F= and =e===f=
=E===f= and =e===F=
,2|Page
A double heterozygous has phase Ef/eF. What are its
parental chromosomes/gametes? - ANSWER-Ef and eF
Which protein is responsible for the initial denaturing of
otic in E.coli? - ANSWER-DnaA
-the DnaA protein initially binds to the 9-mer repeats in
otic. The bound couple of DnaA proteins bend the DNA
and stimulates denaturation of the AT-rich 13-mer region.
In a nucleotide, to which carbon are the phosphates and
base attached? - ANSWER-5' phosphate- 1' base
,3|Page
- in nucleotides, the phosphate group is attached to the 5'
carbon and the base is attached to the 1' carbon
reaction ingredients - ANSWER-1. DNA template with
region to be copied (amplified)
2. dkeoxynuceotide triphosphate= dNTPs (dCTP, dTTP,
dATP, dGTP)
3. polymerase (usually Taq)
4. aqueous buffer (including MgCl₂)
5.DNA primers
6. fluorescently labeled dideoxynucleotide triphosphate=
ddNTPs
, 4|Page
Which of the following is the correct sequence of steps in
a typical PCR sequence - ANSWER-denaturation, primer
annealing, primer extension
Denaturation - ANSWER-reaction is heated to approx. 95
degrees C to separate DNA
Primer annealing - ANSWER-reaction is heating cooled to
45-68 degrees C to allow primers to bind to template
strand
Primer extension - ANSWER-reaction is heated to approx.
72 degrees C for primer extension (synthesis of target
DNA)
