1|Page
BIO 340 MIDTERM EXAM NEWEST 2025/2026
COMPLETE QUESTIONS AND 100%ANSWERS
(VERIFIED AND GRADED A+)
A bacterial enzyme that cuts dna within a short, specific
sequence and is used in many applications by the
molecular biologist is known as: - answer-restriction
enzymes
Which of the following is not needed in order to amplify a
dna fragment by pcr - answer-knowledge of the complete
nucleotide sequence of the fragment to be amplified
Which of the following best describes the complete
sequence of steps occurring every cycle of pcr? - answer-
the primers hybridize to the target dna
,2|Page
Which is the function of dideoxynucelotides in sanger dna
sequencing? - answer-they stop synthesis at a specific
site, so the base at that site can be determined
Illumina next generation sequencing uses... - answer-light
detection after each round of synthesis
Which of the following is an advantage of rna sequencing
over microarray analysis when looking at the mrna
expression levels within a population of cells? - answer-
you can quantify all the rna, rather than just the previously
defines transcripts genes that you have probes for
, 3|Page
Which of the following methods provides the most
sensitive and accurate way of measuring and comparing
mrna levels of a small set of genes in two different tissues.
The genes are expressed at relatively low levels in these
tissues, and do not necessarily code for protien - answer-
rt-pcr
Which of the following techniques would you use to see
where a particular rna molecular localized within a cell
after you edited its 3'utr - answer-in situ hybridization
Which of the following techniques would be the most
appropriate use to see when activation of a particular
promoter causes a protein to be translated ? - answer-
reporter gene
BIO 340 MIDTERM EXAM NEWEST 2025/2026
COMPLETE QUESTIONS AND 100%ANSWERS
(VERIFIED AND GRADED A+)
A bacterial enzyme that cuts dna within a short, specific
sequence and is used in many applications by the
molecular biologist is known as: - answer-restriction
enzymes
Which of the following is not needed in order to amplify a
dna fragment by pcr - answer-knowledge of the complete
nucleotide sequence of the fragment to be amplified
Which of the following best describes the complete
sequence of steps occurring every cycle of pcr? - answer-
the primers hybridize to the target dna
,2|Page
Which is the function of dideoxynucelotides in sanger dna
sequencing? - answer-they stop synthesis at a specific
site, so the base at that site can be determined
Illumina next generation sequencing uses... - answer-light
detection after each round of synthesis
Which of the following is an advantage of rna sequencing
over microarray analysis when looking at the mrna
expression levels within a population of cells? - answer-
you can quantify all the rna, rather than just the previously
defines transcripts genes that you have probes for
, 3|Page
Which of the following methods provides the most
sensitive and accurate way of measuring and comparing
mrna levels of a small set of genes in two different tissues.
The genes are expressed at relatively low levels in these
tissues, and do not necessarily code for protien - answer-
rt-pcr
Which of the following techniques would you use to see
where a particular rna molecular localized within a cell
after you edited its 3'utr - answer-in situ hybridization
Which of the following techniques would be the most
appropriate use to see when activation of a particular
promoter causes a protein to be translated ? - answer-
reporter gene
