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Advanced Nutrition/Human Metabolism Exam

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What are the coenzyme roles of folate? - answer-Accepts single or one carbon groups Involved in metabolism of histidine, serine, glycine and methionine ***Know figure 9.30, pg. 349 for test*** Purine & pyrimidine synthesis and nucleotide metabolism How does folate interact with vitamin B12? - answer-They have a synergistic relationship. W/o vitamin B12 the methyl group cannot be removed from 5-methyl THF and falls into the methyl-folate trap. 5-methyl THF is required for methionine synthesis from homocysteine. With adequate B12 status, the THF resulting from methionine synthesis can be used to make the forms of folate needed for DNA synthesis What disease is associated w/ a deficiency in folate? - answer-Megaloblastic macrocytic anemia (1 month w/o folate. Treatment = 1-5 mg/day) What two enzymatic reactions is vitamin B12 involved in? - answer-1. Conversion of homocysteine to methionine (needs methylcobalamin) 2. Conversion of L-methylmalonyl CoA to succinyl CoA (needs adenosylcobalamin) What disease is vitamin B12 deficiency associated with? - answer-Megaloblastic macrocytic anemia (pernicious anemia, low blood leukocytes and thrombocytes, skin pallor, fatigue, shortness of breath and palpitations) ~ neurological damage can be permanent What coenzyme roles does vitamin B6 play? - answer-1. Transamination (transfer of an amino group to an a-ketoacid) 2. Deamination (amino acid is removed and released as ammonia or ammonium ion) 3. Synthesis of neurotransmitters, amino acids (cysteine, selenocysteine, glycine), niacin, heme, sphingolipids, select PUFAs, carnitine and taurine 4. Glycogen degradation What non-coenzyme roles does vitamin B6 play? - answer-Modulation of gene expression What is a cofactor? What is a coenzyme? - answer-Cofactor - minerals (zinc, iron, copper, potassium, magnesium, manganese) involved in chemical reactions Coenzymes - vitamins (thiamin, riboflavin, niacin, pantothenic acid, biotin) involved in chemical reactions

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;Advanced Nutrition/Human
Metabolism Exam
With answers



60 mg of tryptophan equals how many nitrogen equivalents (NEs) - answer-1 mg
NE

1 gram of complete protein equals how many mg of tryptophan - answer-10 mg
tryptophan

What two ways does vitamin C enhance the absorption of nonheme iron? -
answer-1. reduces iron from a ferric state (Fe3+) to a ferrous state (Fe2+)
2. forms a soluble complex w/ the reduced iron in the alkaline pH of the small
intestine

What is the formula for calculating dietary folate equivalents (DFEs) - answer-DFE
= micrograms of food folate + (1.7 x micrograms folic acid)

Major food sources for vitamin B1 (thiamin) - answer-Animal meats (especially
pork), salmon, legumes, whole and enriched grains, yeast, wheat germ and soy
milk

Major food sources for vitamin B2 (riboflavin) - answer-Dairy products (milk,
yogurt, cheese), eggs, animal meats, legumes, green vegetables (spinach)

Major food sources for vitamin B3 (niacin) - answer-Beef, pork, fish, poultry,
whole and enriched grains, fortified cereals, seeds and legumes (also in coffee
and tea)

Major food sources for vitamin B5 (pantothenic acid) - answer-Found everywhere
(pantos) but good sources are animal meats, egg yolk, yogurt, legumes, whole
grain cereals, potatoes, mushrooms and avocados

Major food sources of vitamin B7
(biotin) - answer-Beef liver, soy beans and egg yolk.

Major food sources of vitamin B9
(folate) - answer-Mushrooms, spinach, broccoli, asparagus, Brussels sprouts,
turnips, collard greens, okra, legumes, strawberries and oranges and liver

, Major food sources of folic acid - answer-Fortified cereals, breads and grain
products

Major food sources of vitamin B12 (cobalamin) - answer-Meat and meat products,
fortified cereals and soy milk.

None of the water soluble vitamins are stored in the body except for which one? -
answer-Vitamin B12 & B6

All of the water soluble vitamins are absorbed via active diffusion (except in high
concentrations = passive) except for which one? - answer-Vitamin B6

Which two ways are water soluble vitamins found in the blood stream? - answer-
Free form or with a protein transporter (albumin)

Which water soluble vitamins are not associated w/ a deficiency disease? -
answer-Biotin, pantothenic acid, vitamin B6 and riboflavin

Which of the water soluble vitamins have a UL? - answer-Vitamin C, vitamin B6,
niacin and folate

Vitamin C functions? - answer-Antioxidant; and hydroxylating reactions involved
in the synthesis of collagen, carnitine, tyrosine and neurotransmitters
(norepinephrine and serotonin)

Vitamin C in synthesis of collagen. What is the mechanism of action? - answer-3
hydroxylation reactions are required. Relates to the iron cofactor - the iron
cofactor in the enzymes is oxidized during the prolyl (3- and 4-) and lysyl
hydroxylase reactions and vitamin C functions as the reductant, reducing iron
back to the ferrous (2+) state

Vitamin C in synthesis of carnitine. What is the mechanism of action? - answer-2
hydroxylation reactions. Vitamin C functions as a reducing agent, reducing the
iron atom from the ferric state (Fe3+) back to the ferrous state (2+) for the
reactions catalyzed by trimethyllysine hydroxylase and 4-butyrobetaine
hydroxylase.

Vitamin C in synthesis of tyrosine. What is the mechanism of action? - answer-
Regenerates tetrahydrobiopterin (co-substrate for hydroxylation of phenylalanine
by the iron-dependent enzyme phenylalanine hydroxylase)

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