2025/2026/GCSU Bachoon Microbiology Lab Final
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Compare the effectiveness of moist heat (boiling,
autoclaving, pasteurization) and dry heat. - ANSWER-
Moist heat kills microbes by denaturing enzymes.
An autoclave is an example of moist heat sterilization and
is the most effective method of moist heat sterilization.
Identify chemical sterilizer - ANSWER-plasma- free
radicals destroy microbes, use on tubular instruments
, supercritical fluids- CO2 with gaseous and liquid
properties, use on medical implants
peroxygens- oxidizing agents, use on contaminated
surfaces and food package
-->O3, H2O2 and peracetic acid
Explain how the type of microbe affects the control of
microbial growth. - ANSWER-biocides tend to be more
effective against gram-positive bacteria- external
lipopolysaccharide layer of gram-negative bacteria, makes
them resistant ;