PASS QUESTIONS AND DEFINITIVE ANSWERS
Hard tissue
Warm microtome
Loose block/blade
Too much blade tilt
Cause of wash boarding (venetian blinds)
Soak block
Tighten parts
Decrease blade tilt
Solution for wash boarding (venetian blinds)
Over dehydration
Dull blade
Too much blade tilt
Cutting too rapidly
Define cause of microvibrations (microchatter)
,Check processing schedule
Replace/Move blade
Decrease blade tilt
Cut slower
Solution for microvibrations (microchatter)
Too little blade tilt
Loose or worn microtome parts
Hard tissue
Cause of thick & thin sections
Increase blade tilt
Tighten parts
Soak block
Solution for thick & thin sections
Dull blade
Dirty blade
Too little blade tilt
,Cutting to fast
Room temperature too warm
Cause of compressed (wrinkled) sections
Move/replace blade
Clean blade
Increase blade tilt
Cut slower
Cool block or adjust room temperature
Solutions for compressed (wrinkled) sections
Knick on blade
Hard particle in block
Cause of lengthwise scratches on section
Avoid staples and sutures
Surface decal
Soak block
Move/replace blade
, Solution for lengthwise scratches on section
Static electricity
Cause of flying sections
Breathe on sections
Ground microtome to a water pipe
Solution for flying sections
Material too hard
Cause of scratching noise when cutting
Soak block
Surface decal block
Solution for scratching noise when cutting
Incomplete dehydration or clearing