MB ASCP CONNECT EXAM PRACTICE QUESTIONS
WITH CORRECT ANSWERS GRADED A+ NEW
MODIFIED
Which of the following is not involved in the splicing reaction?
a. 5' splice site
b. Hairpin loops
c. Branch A point
d. 3' splice site --CORRECT ANSWER--Hairpin loops
Which of the following statements are characteristics of the melt curve
analysis? (hint: more than one answer)
a. At the melting point, the probe separates from the target strand and
fluorescence rapidly decreases.
b. The melting temperature of double stranded DNA depends on its base
composition and length.
c. All PCR products for a specific primer pair should have the same melting
temperature.
d. When hybridization probes are utilized, the temperature is incrementally
decreased while fluorescence is monitored. --CORRECT ANSWER--At the
Page 1 of 105
,melting point, the probe separates from the target strand and fluorescence
rapidly decreases.
The melting temperature of double stranded DNA depends on its base
composition and length.
All PCR products for a specific primer pair should have the same melting
temperature.
In the field of molecular diagnostics, which one of the following genes is
responsible for the synthesis of DNA, promote cell division, and inhibit cell
death?
a. Proto-oncogenes
b. Tumor suppressor genes
c. Oncogenes
d. Mitochondrial genes --CORRECT ANSWER--Proto-oncogenes
Microsatellite instability is best described as:
a. The ability of a gene with repeating elements of 8-10 base pairs to randomly
mutate
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,b. Contraction or expansion of the genome caused by frameshift mutations
(deletions or insertions) in elements of the genome consisting of a repeating
sequence of 1-3 base pairs
c. Repetitive elements in the genome with the ability to self-prime, thus creating
additional copies of genetic material at the allele loci
d. Random gene rearrangement between repeating elements of the same size on
different chromosomes resulting in different size gene products --CORRECT
ANSWER--Contraction or expansion of the genome caused by frameshift
mutations (deletions or insertions) in elements of the genome consisting of a
repeating sequence of 1-3 base pairs
Purines and pyrimidines differ from each other in that:
a. Purines are found RNA; pyrimidines are found in DNA
b. There's no difference between purines and pyrimidines
c. Pyrimidines have two rings; purines have one ring
d. Purines have two rings; pyrimidines have one ring --CORRECT ANSWER--
Purines have two rings; pyrimidines have one ring
TIP: Reciprocals of each other.
Purine, shorter word, longer rings.
Pyrimidine, longer word, shorter ring.
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, Which of the following molecular methodologies would be the best for
detecting a trinucleotide repeat disorder such as Huntington's disease?
a. Heteroduplex analysis
b. Variable number tandem repeat analysis
c. Single strand conformation polymorphism analysis
d. Reverse-Transcriptase PCR --CORRECT ANSWER--Variable number
tandem repeat analysis
Locus Test C M PF1 PF2
A1 12/15 12 15/17 12/15
B2 13 13/13 13/15 14/15
C3 7/8 8 7 7/7
D4 9/11 9/9 10/11 11
E5 6 6/6 5/7 6
Based on the results above, which one of the two possible fathers is the two-
month old's biological father?
a. Possible Father 1
b. Possible Father 2
Page 4 of 105
WITH CORRECT ANSWERS GRADED A+ NEW
MODIFIED
Which of the following is not involved in the splicing reaction?
a. 5' splice site
b. Hairpin loops
c. Branch A point
d. 3' splice site --CORRECT ANSWER--Hairpin loops
Which of the following statements are characteristics of the melt curve
analysis? (hint: more than one answer)
a. At the melting point, the probe separates from the target strand and
fluorescence rapidly decreases.
b. The melting temperature of double stranded DNA depends on its base
composition and length.
c. All PCR products for a specific primer pair should have the same melting
temperature.
d. When hybridization probes are utilized, the temperature is incrementally
decreased while fluorescence is monitored. --CORRECT ANSWER--At the
Page 1 of 105
,melting point, the probe separates from the target strand and fluorescence
rapidly decreases.
The melting temperature of double stranded DNA depends on its base
composition and length.
All PCR products for a specific primer pair should have the same melting
temperature.
In the field of molecular diagnostics, which one of the following genes is
responsible for the synthesis of DNA, promote cell division, and inhibit cell
death?
a. Proto-oncogenes
b. Tumor suppressor genes
c. Oncogenes
d. Mitochondrial genes --CORRECT ANSWER--Proto-oncogenes
Microsatellite instability is best described as:
a. The ability of a gene with repeating elements of 8-10 base pairs to randomly
mutate
Page 2 of 105
,b. Contraction or expansion of the genome caused by frameshift mutations
(deletions or insertions) in elements of the genome consisting of a repeating
sequence of 1-3 base pairs
c. Repetitive elements in the genome with the ability to self-prime, thus creating
additional copies of genetic material at the allele loci
d. Random gene rearrangement between repeating elements of the same size on
different chromosomes resulting in different size gene products --CORRECT
ANSWER--Contraction or expansion of the genome caused by frameshift
mutations (deletions or insertions) in elements of the genome consisting of a
repeating sequence of 1-3 base pairs
Purines and pyrimidines differ from each other in that:
a. Purines are found RNA; pyrimidines are found in DNA
b. There's no difference between purines and pyrimidines
c. Pyrimidines have two rings; purines have one ring
d. Purines have two rings; pyrimidines have one ring --CORRECT ANSWER--
Purines have two rings; pyrimidines have one ring
TIP: Reciprocals of each other.
Purine, shorter word, longer rings.
Pyrimidine, longer word, shorter ring.
Page 3 of 105
, Which of the following molecular methodologies would be the best for
detecting a trinucleotide repeat disorder such as Huntington's disease?
a. Heteroduplex analysis
b. Variable number tandem repeat analysis
c. Single strand conformation polymorphism analysis
d. Reverse-Transcriptase PCR --CORRECT ANSWER--Variable number
tandem repeat analysis
Locus Test C M PF1 PF2
A1 12/15 12 15/17 12/15
B2 13 13/13 13/15 14/15
C3 7/8 8 7 7/7
D4 9/11 9/9 10/11 11
E5 6 6/6 5/7 6
Based on the results above, which one of the two possible fathers is the two-
month old's biological father?
a. Possible Father 1
b. Possible Father 2
Page 4 of 105
