age 1 of 58
MLS ASCP MICROBIOLOGY FINAL EXAM STUDY GUIDE
2025/2026 ACCURATE QUESTIONS AND CORRECT
DETAILED ANSWERS WITH RATIONALES || 100%
GUARANTEED PASS <BRAND NEW VERSION>
How would you differentiate Yersinia species
......ANSWER........Answer: motility (motile at room temp not 37) -
All species except Y. pestis are NON-MOTILE at 37 and MOTILE
at 25 C (room temperature) Y. pestis non-motile at both
JEMBEC system ......ANSWER........is a transport and inoculation
medium used for direct plating of specimens for N gonorrhoeae.
Chocolate based selective medium is inoculated with the
specimen. This is placed in an impermeable plastic bag with a
,age 2 of 58
bicarbonate-sodium citrate pellet to generate a carbon dioxide
rich environment.
Moraxella osloensis ......ANSWER........is a gram-negative
coccobacillus that is often plump and occurs in pairs and
demonstrates a morphology similar to Neisseria
1 : 10 ratio ......ANSWER........The proper blood-to-broth ratio for
blood cultures to reduce the antibacterial effects of serum
Beta-lactamase ......ANSWER........Produced by a penicillin-
resistant N. gonorrhoeae
Indole test ......ANSWER........- organism's ability to produce
indole from deamination of tryptophan
,age 3 of 58
Martin Lewis agar ......ANSWER........Chocolate agar base
containing vancomycin, colistin, anisomycin, and trimethoprim for
better inhibition of gram-positive organisms and candida.
SPS ......ANSWER........used as anticoagulant in blood cultures to
inactivate neutrophils and components of serum complement
Blood culture ......ANSWER........Typhoid fever can be confirmed
by this culture
Regan-Lowe agar ......ANSWER........best medium for culture for
Bordetella pertussis
synovial infections ......ANSWER........Anaerobic susceptibility
testing is helpful
Schlichter test ......ANSWER........is used to monitor bactericidal
activity during antimicrobic therapy in cases of endocaditis
, age 4 of 58
Extra-intestinal isolates of Salmonella ......ANSWER........should be
tested for resistance to nalidixic acid and if resistant report the
ciprofloxacin as resistant
Gentamicin ......ANSWER........antimicrobial resistance occurs from
enzymatic drug modification
Enterococcus infection ......ANSWER........need 2 antimicrobial
agents, penicillin derivative and an aminoglycoside
pulsed-field gel electrophoresis ......ANSWER........commonly used
method to determine the relatedness of 2 or more bacterial
strains
Micrococcus ......ANSWER........is modified oxidase positive,
bacitracin (0.04U) susceptible and resistant to lysotaphin
MLS ASCP MICROBIOLOGY FINAL EXAM STUDY GUIDE
2025/2026 ACCURATE QUESTIONS AND CORRECT
DETAILED ANSWERS WITH RATIONALES || 100%
GUARANTEED PASS <BRAND NEW VERSION>
How would you differentiate Yersinia species
......ANSWER........Answer: motility (motile at room temp not 37) -
All species except Y. pestis are NON-MOTILE at 37 and MOTILE
at 25 C (room temperature) Y. pestis non-motile at both
JEMBEC system ......ANSWER........is a transport and inoculation
medium used for direct plating of specimens for N gonorrhoeae.
Chocolate based selective medium is inoculated with the
specimen. This is placed in an impermeable plastic bag with a
,age 2 of 58
bicarbonate-sodium citrate pellet to generate a carbon dioxide
rich environment.
Moraxella osloensis ......ANSWER........is a gram-negative
coccobacillus that is often plump and occurs in pairs and
demonstrates a morphology similar to Neisseria
1 : 10 ratio ......ANSWER........The proper blood-to-broth ratio for
blood cultures to reduce the antibacterial effects of serum
Beta-lactamase ......ANSWER........Produced by a penicillin-
resistant N. gonorrhoeae
Indole test ......ANSWER........- organism's ability to produce
indole from deamination of tryptophan
,age 3 of 58
Martin Lewis agar ......ANSWER........Chocolate agar base
containing vancomycin, colistin, anisomycin, and trimethoprim for
better inhibition of gram-positive organisms and candida.
SPS ......ANSWER........used as anticoagulant in blood cultures to
inactivate neutrophils and components of serum complement
Blood culture ......ANSWER........Typhoid fever can be confirmed
by this culture
Regan-Lowe agar ......ANSWER........best medium for culture for
Bordetella pertussis
synovial infections ......ANSWER........Anaerobic susceptibility
testing is helpful
Schlichter test ......ANSWER........is used to monitor bactericidal
activity during antimicrobic therapy in cases of endocaditis
, age 4 of 58
Extra-intestinal isolates of Salmonella ......ANSWER........should be
tested for resistance to nalidixic acid and if resistant report the
ciprofloxacin as resistant
Gentamicin ......ANSWER........antimicrobial resistance occurs from
enzymatic drug modification
Enterococcus infection ......ANSWER........need 2 antimicrobial
agents, penicillin derivative and an aminoglycoside
pulsed-field gel electrophoresis ......ANSWER........commonly used
method to determine the relatedness of 2 or more bacterial
strains
Micrococcus ......ANSWER........is modified oxidase positive,
bacitracin (0.04U) susceptible and resistant to lysotaphin
