Portage Learning: Microbiology Final Exam Set Already
Passed
/. You want to observe the movement of microorganisms that are present in a sample of
cheek cells obtained on a patient. Briefly explain the technique that you would use and
how it is performed. - Answer-✅A wet mount stain is indicated. A small liquid culture
(usually just a drop) containing a microorganism of interest is prepared, added to a
slide, and then covered with a glass coverslip. The coverslip is present to both protect
the objective and the specimen while also holding the microorganism in place. Wet
mounts are used to observe the motility and behavior of an organism.
/.briefly explain why a researcher would use the simple stain technique. How is it
preformed and what does it identify - Answer-✅it uses a solution of positively charged
dye, which binds to and stains the negatively changed membrane of the microorganism.
This technique can show size, shape, and arrangement of cells
/.What coronavirus-associated infection gave rise to quarantining and travel restrictions
in 2003? - Answer-✅SARS, 2020 was COVID-19
/.Match each type of agar plate to the corresponding general
characteristics of each.
LB Agar
Trypticase Soy Agar
MacConkey Agar
Mannitol Salt Agar - Answer-✅LB Agar - Also known as lysogeny broth, it is a multi-
purpose media capable of growing a wide variety of microorganisms
Trypticase Soy Agar - A multi-purpose media capable of growing a wide variety of
microorganisms
MacConkey Agar - Pale red in color and is both selective and differential media. The
presence of crystal violet and bile salts in its formulation restricts Gram- positive
bacterial growth. As such, only Gram- negative microbes can be grown.
Mannitol Salt Agar - This Agar is selective for Gram-positive bacteria and can
differentiate members of the Staphylococci family
/.True or False: Leprosy can move through the blood to other areas including the brain,
spine, and kidneys. - Answer-✅False: TB is primarily considered a respiratory infection,
but once it fully colonizes the lungs, TB can move through the blood to other areas
including the brain, spine, and kidneys.
/.True or False: Proteins can function to catalyze biochemical processes and play a role
in structure, but they cannot enable movement. - Answer-✅False. Proteins can function
, to catalyze biochemical processes, play a role in structure, and they can enable
movement.
/.True or false: if false, make it true
Lipids are primarily composed of __________ hydrocarbons
FALSE: Lipids are primarily composed of ___________ hydrocarbons - Answer-
✅Lipids are primarily composed of hydrophilic hydrocarbons
FALSE: Lipids are primarily composed of hydrophobic hydrocarbons
/.Would one of the SARS-coV-2 vaccines give immunity against SARS or MERS
infection? Explain - Answer-✅SARS: the vaccine only has biological material for the
specific spike protein which would be SARS, hence the name of the vaccine. There are
unique vaccines to different strands on infections
/.You are working with a patient who claims a friend "got the COVID-19 infection from
the vaccine". Explain why the viral vector vaccine used to prevent the COVID-19
infection cannot transmit the actual infection. - Answer-✅The genome in the viral vector
only encodes for a spike protein and not the other components of the virus. Therefore,
new virions or virus particles cannot be made or replicated.
/.Match each type of microscopy with the correct description.
Bright Field Microscope
Fluorescence Microscope
Phase Contrast Microscope
Electron Microscope
Confocal Microscope
Dark Field Microscope - Answer-✅Bright Field Microscope - The simplest form of light,
or optical, microscopes. Light, most often emitted from a standard halogen
bulb, enters the microscope from the base (bottom) and is reflected via mirrors towards
the sample.
Fluorescence Microscope - Incoming light is in the form of the ultraviolet (UV) spectrum
Phase Contrast Microscope - Can provide detailed images of live cells without staining
Electron Microscope - Use of beams of electrons (rather than light) to increase its
resolution capacity to less than 1 nm - that's 200x.
Confocal Microscope - Combines the usefulness of fluorescence microscopy with the
ability to visualize cells in 3-D
Dark Field Microscope - The microscope reflects light off of the specimen at an angle
rather than light passing through the specimen
/.T or F: dark reactions can occur in the presence or absence of light - Answer-✅True
/.Which of the following reactions is an example of a hydrolase?
a. A + B → A-B
b. A-B + H2O → A-OH + B-H
c. A— + B →A + B—
Passed
/. You want to observe the movement of microorganisms that are present in a sample of
cheek cells obtained on a patient. Briefly explain the technique that you would use and
how it is performed. - Answer-✅A wet mount stain is indicated. A small liquid culture
(usually just a drop) containing a microorganism of interest is prepared, added to a
slide, and then covered with a glass coverslip. The coverslip is present to both protect
the objective and the specimen while also holding the microorganism in place. Wet
mounts are used to observe the motility and behavior of an organism.
/.briefly explain why a researcher would use the simple stain technique. How is it
preformed and what does it identify - Answer-✅it uses a solution of positively charged
dye, which binds to and stains the negatively changed membrane of the microorganism.
This technique can show size, shape, and arrangement of cells
/.What coronavirus-associated infection gave rise to quarantining and travel restrictions
in 2003? - Answer-✅SARS, 2020 was COVID-19
/.Match each type of agar plate to the corresponding general
characteristics of each.
LB Agar
Trypticase Soy Agar
MacConkey Agar
Mannitol Salt Agar - Answer-✅LB Agar - Also known as lysogeny broth, it is a multi-
purpose media capable of growing a wide variety of microorganisms
Trypticase Soy Agar - A multi-purpose media capable of growing a wide variety of
microorganisms
MacConkey Agar - Pale red in color and is both selective and differential media. The
presence of crystal violet and bile salts in its formulation restricts Gram- positive
bacterial growth. As such, only Gram- negative microbes can be grown.
Mannitol Salt Agar - This Agar is selective for Gram-positive bacteria and can
differentiate members of the Staphylococci family
/.True or False: Leprosy can move through the blood to other areas including the brain,
spine, and kidneys. - Answer-✅False: TB is primarily considered a respiratory infection,
but once it fully colonizes the lungs, TB can move through the blood to other areas
including the brain, spine, and kidneys.
/.True or False: Proteins can function to catalyze biochemical processes and play a role
in structure, but they cannot enable movement. - Answer-✅False. Proteins can function
, to catalyze biochemical processes, play a role in structure, and they can enable
movement.
/.True or false: if false, make it true
Lipids are primarily composed of __________ hydrocarbons
FALSE: Lipids are primarily composed of ___________ hydrocarbons - Answer-
✅Lipids are primarily composed of hydrophilic hydrocarbons
FALSE: Lipids are primarily composed of hydrophobic hydrocarbons
/.Would one of the SARS-coV-2 vaccines give immunity against SARS or MERS
infection? Explain - Answer-✅SARS: the vaccine only has biological material for the
specific spike protein which would be SARS, hence the name of the vaccine. There are
unique vaccines to different strands on infections
/.You are working with a patient who claims a friend "got the COVID-19 infection from
the vaccine". Explain why the viral vector vaccine used to prevent the COVID-19
infection cannot transmit the actual infection. - Answer-✅The genome in the viral vector
only encodes for a spike protein and not the other components of the virus. Therefore,
new virions or virus particles cannot be made or replicated.
/.Match each type of microscopy with the correct description.
Bright Field Microscope
Fluorescence Microscope
Phase Contrast Microscope
Electron Microscope
Confocal Microscope
Dark Field Microscope - Answer-✅Bright Field Microscope - The simplest form of light,
or optical, microscopes. Light, most often emitted from a standard halogen
bulb, enters the microscope from the base (bottom) and is reflected via mirrors towards
the sample.
Fluorescence Microscope - Incoming light is in the form of the ultraviolet (UV) spectrum
Phase Contrast Microscope - Can provide detailed images of live cells without staining
Electron Microscope - Use of beams of electrons (rather than light) to increase its
resolution capacity to less than 1 nm - that's 200x.
Confocal Microscope - Combines the usefulness of fluorescence microscopy with the
ability to visualize cells in 3-D
Dark Field Microscope - The microscope reflects light off of the specimen at an angle
rather than light passing through the specimen
/.T or F: dark reactions can occur in the presence or absence of light - Answer-✅True
/.Which of the following reactions is an example of a hydrolase?
a. A + B → A-B
b. A-B + H2O → A-OH + B-H
c. A— + B →A + B—
