BISC 101 LAB EXAM QUESTIONS AND ANSWERS GRADED A+
Serological Pipette - Answers Used to accurately dispense volumes of liquids
pasteur pipette - Answers Used to dispense liquids when accuracy is less important , and to
gently mix liquid mixtures by pipetting in and out
Preparing wet mounts on glass slides - Answers To spread a sample thin enough ( between a
glass slide and a glass cover slip) to view clearly under a microscope
A simple chemical test : Using Iodine to test for starch - Answers To determine whether a
sample contains starch
Starch= plant/veggies
No starch = animals
Cheesecloth and funnel filter instructions - Answers Used to filter out larger solids while
allowing liquids and smaller particles ( eg. Cells, organelles, etc) to pass through
how do molecules pass the membrane - Answers active/facilitated transport, selective
permeability of lipid bilayer, and concentration gradient
compound microscope - Answers Used to view objects at 40x 100x or 400x magnification - to
see cells
spectrophotometer - Answers Used to measure how much light ( of each specific
wavelength/colour) is absorbed by a sample. A prism is used to split the light, so that you can
test one wavelength at a time across a wider range of possible wavelengths
eppendorf/IEC centrifuge - Answers Used to separate the particles within a mixture into layers
according to their relative densities, by spinning the mixture at extremely high speeds. The
densest particles are found at the bottom of the table
Independent variable - Answers The variable that is manipulated and its effect on the dependent
variable is measured
Hypertonic - Answers When the concentration of solute is greater outside than inside, water
flows out= causes cell to shrivel and shrink in size
Hypotonic - Answers When the concentration of solute is greater inside the cell than outside,
water flows in= causes cell to swell and burst
isotonic - Answers when the concentration of both the solute and solvent is equal so theres no
net movement
What happens when you put a cell into a hypotonic solution? - Answers fills to almost bursting
, what happens when you put a cell into a hypertonic solution - Answers shrivels
what happens when you put a cell into an isotonic solution - Answers normal
the cell membrane is permeable to - Answers Small hydrophobic molecules ( ex. O2, CO2 , N2 ,
Benzene)
Small uncharged molecules ( ex. H2O, glycerol)
the cell membrane is not permeable to - Answers Large, uncharged molecules (ex. Glucose,
sucrose)
Ions (ex. Cl-, K + , Na+)
why does Increasing the temperature increases the membrane permeability - Answers o When
raising the temperature, its fluidity increases, the atoms move faster including those that make
up the phospholipid bilayers .
o This causes the membrane to be more fluid since the phospholipids are also moving and that
it can be easily penetrated by other molecules
Why do you think a solute's solubility affects its ability to cross the RBC membrane ? - Answers
o A solutes solubility in oil affect its ability to cross the RBC membrane helps determine its
polarity. Since Water is polar and oil is non polar , it means that the molecules are not attracted
to each other. Thus, the more soluble in oil it is, the more polar the solute is.
What color are the P atoms in 3D molecule of DNA? what is phosphorus bonded to, to make a
phosphate group? - Answers - Purple
attached to the 5 carbon sugar at the 5th carbon (opposite to nitrogenous base /react-text
- it is made up of a P with 4O bonded to it to make a phosphorus group
What is the colour of the nitrogen atoms, - Answers -Blue
-N is bonded to the first carbon of the 5 carbon molecule
what bond is in purine and pyrimidines - Answers -A bonded to T by 2 hydrogen bonds (double
bond)
-G bonded to C by 3 hydrogen bonds (triple bond)
what is N bonded to from nitrogenous base? - Answers -It forms either A,T,C, or G base
-(A,G) = purine base (two rings)
-(T,C) = pyrimidine base (one ring)
Serological Pipette - Answers Used to accurately dispense volumes of liquids
pasteur pipette - Answers Used to dispense liquids when accuracy is less important , and to
gently mix liquid mixtures by pipetting in and out
Preparing wet mounts on glass slides - Answers To spread a sample thin enough ( between a
glass slide and a glass cover slip) to view clearly under a microscope
A simple chemical test : Using Iodine to test for starch - Answers To determine whether a
sample contains starch
Starch= plant/veggies
No starch = animals
Cheesecloth and funnel filter instructions - Answers Used to filter out larger solids while
allowing liquids and smaller particles ( eg. Cells, organelles, etc) to pass through
how do molecules pass the membrane - Answers active/facilitated transport, selective
permeability of lipid bilayer, and concentration gradient
compound microscope - Answers Used to view objects at 40x 100x or 400x magnification - to
see cells
spectrophotometer - Answers Used to measure how much light ( of each specific
wavelength/colour) is absorbed by a sample. A prism is used to split the light, so that you can
test one wavelength at a time across a wider range of possible wavelengths
eppendorf/IEC centrifuge - Answers Used to separate the particles within a mixture into layers
according to their relative densities, by spinning the mixture at extremely high speeds. The
densest particles are found at the bottom of the table
Independent variable - Answers The variable that is manipulated and its effect on the dependent
variable is measured
Hypertonic - Answers When the concentration of solute is greater outside than inside, water
flows out= causes cell to shrivel and shrink in size
Hypotonic - Answers When the concentration of solute is greater inside the cell than outside,
water flows in= causes cell to swell and burst
isotonic - Answers when the concentration of both the solute and solvent is equal so theres no
net movement
What happens when you put a cell into a hypotonic solution? - Answers fills to almost bursting
, what happens when you put a cell into a hypertonic solution - Answers shrivels
what happens when you put a cell into an isotonic solution - Answers normal
the cell membrane is permeable to - Answers Small hydrophobic molecules ( ex. O2, CO2 , N2 ,
Benzene)
Small uncharged molecules ( ex. H2O, glycerol)
the cell membrane is not permeable to - Answers Large, uncharged molecules (ex. Glucose,
sucrose)
Ions (ex. Cl-, K + , Na+)
why does Increasing the temperature increases the membrane permeability - Answers o When
raising the temperature, its fluidity increases, the atoms move faster including those that make
up the phospholipid bilayers .
o This causes the membrane to be more fluid since the phospholipids are also moving and that
it can be easily penetrated by other molecules
Why do you think a solute's solubility affects its ability to cross the RBC membrane ? - Answers
o A solutes solubility in oil affect its ability to cross the RBC membrane helps determine its
polarity. Since Water is polar and oil is non polar , it means that the molecules are not attracted
to each other. Thus, the more soluble in oil it is, the more polar the solute is.
What color are the P atoms in 3D molecule of DNA? what is phosphorus bonded to, to make a
phosphate group? - Answers - Purple
attached to the 5 carbon sugar at the 5th carbon (opposite to nitrogenous base /react-text
- it is made up of a P with 4O bonded to it to make a phosphorus group
What is the colour of the nitrogen atoms, - Answers -Blue
-N is bonded to the first carbon of the 5 carbon molecule
what bond is in purine and pyrimidines - Answers -A bonded to T by 2 hydrogen bonds (double
bond)
-G bonded to C by 3 hydrogen bonds (triple bond)
what is N bonded to from nitrogenous base? - Answers -It forms either A,T,C, or G base
-(A,G) = purine base (two rings)
-(T,C) = pyrimidine base (one ring)
