ANSWERS | NEW 2025/2026 UPDATE | 100 CORRECT
Oswald Avery showed that Answer - DNA is responsible for bacterial
transformations.
Which of the following refers to manipulating a piece of DNA and putting it into
a plasmid for the purposes of gene studies, protein expression, and gene
therapy? Answer - Recombinant DNA technology
Employers must inform employees and train them in the safe handling
potentially harmful chemicals. This is required by Answer - OSHA
Research labs that handle pathogenic organisms for which there is no vaccine
that can cause diseases which are generally untreable and fatal are assigned a
rating of Answer - BSL-4
Where should you open bottles that contain volatile chemicals such as HCl or
SDS? Answer - In the fume hood.
Which type of gloves offer the least amount of protection from chemical/
biological hazards? Answer - Plastic
A device used to clean glassware and sterilize media using heat and pressure is
a(n) Answer - autoclave
, The least accurate way to transfer a specific volume would be with a Answer -
Pasteur (transfer) pipette
A Mohr pipette has a thinner tip, which is beneficial when using the pipette to
Answer - dispense specific volumes several times from a single aspiration
Some tips have cotton swabs in them to Answer - prevent contamination of
the pipettes and samples
You have a 1.5 ml tube of frozen enzyme. Upon removing it from the freezer
you should thaw it and spin it down briefly in a centrifuge. What is the purpose
of this? Answer - To remove any liquid from inside the cap
Why are most centrifuges refrigerated? Answer - To prevent protein
degradation
A type of centrifugation used in the lab to rapidly collect samples in 1.5 ml
tubes is Answer - small bench top centrifugation.
A protocol you are following says to spin a sample in a Sorvall SA-600 at 10,000
rpm. You are using a Sorvall SS-34 rotor, which is much smaller. What must you
do? Answer - Calculate the relative centrifugal field of the same SA-600 to
determine what rpm to use in the SS-34.
When using a pipette, the greatest accuracy is achieved by Answer - holding
the pipette perfectly vertical
with regards to potential destructive ability, which type of centrifuge is most
dangerous and must be balanced within 0.1g? Answer - Ultracentrifuge