AAB MT CHEMISTRY EXAM 2025 WITH
100% ACCURATE ANSWERS
Which of the four elements in proteins differentiated this class of substances from
carbohydrates and lipids? - answers Nitrogen
Which of the proteins is soluble in water? - answers Albumin
How are amino acids joined together to form a chainlike structure? - answers Peptide
bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or
chemical treatment. What does denaturation of proteins refer to? - answers Alteration of
tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - answers Cystinuria
These aminoacidopathies are caused by an enzyme deficiency: - answers Maple syrup
disease, phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - answers Five
Which reagent is employed in the serum protein determination? - answers Biuret
Why should hemolyzed serum not be used for total protein analysis? - answers
Hemoglobin is absorbed at the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction? - answers It is relatively free
from interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - answers Follows Beer's law for a
reasonable range of protein, depends on the presence of at least two peptide linkages,
and is insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
answers There is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - answers Albumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
,What is the A/G ratio? - answers 3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state? - answers Active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10%
beta-globulin, and 45% gamma globulin, then what condition may exist in the patient? -
answers Monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - answers
Carbohydrates
All of the following are glycoproteins: - answers Ceruloplasmin, follicle-stimulating
hormone (FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - answers Cryoglobulin
Which one of the following applies to cryoglobulins? - answers They are temperature-
sensitive proteins
Which of the following statements is true of albumin? - answers It is produced in the
liver and is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - answers
Down syndrome
Which one of the following proteins transports iron? - answers Transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - answers Blood urea
nitrogen (BUN)
By the urease method, urea is enzymatically converted to which end product? - answers
Ammonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxomine method? - answers The method is not measuring ammonia
An elevated creatinine value is most likely to be accompanied by which of these values?
- answers Elevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? - answers Jaffe
The measurement of creatinine is based on the formation of a yellow-red color and
which reagent? - answers Alkaline picrate
,The creatinine clearance test is based on which assumption? - answers Creatinine
passes into the ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration rate?
(GFR) - answers Creatinine
A creatinine clearance test is performed. The 24-hour urine volume is 770 mL; serum
creatinine is 2.0 mg/dL and urine creatinine is 240 mg/dL. What is the clearance,
assuming average body surface area? - answers GFR = ([UCr x V]/SCr)/1440
64 mL/minute = [(240 x 770)/2]/1440
(1440 minutes in 24 hours)
Which disease state is associated with an elevation of serum uric acid? - answers Gout
Which reagent is employed in the alkaline oxidation of uric acid? - answers
Phosphotungstic acid
What does the uricase method for uric acid depend on? - answers UV absorption at 290
nm before/after treatment with uricase
Which one of the following analytes is used as a prognostic indicator for liver failure? -
answers Ammonia
Which disaccharide is composed of two glucose molecules? - answers Maltose
What term is used to describe the process of glycogen degradation? - answers
Glycogenolysis
What is the renal threshold for glucose? - answers 160-180 mg/dL
Which hormone is produced by the beta cells of the islets of Langerhans? - answers
Insulin
Which one of the following hormones is considered a glucocorticoid? - answers Cortisol
A 40-year-old male has a randome glucose of 470 mg/dL. This lab finding is consistent
with which one of the following clinical conditions? - answers Diabetes mellitus
Which one of the following diseases is associated with hypoglycemia? - answers von
Gierke's disease
What is the specimen of choice for glucose determinations? - answers Fluorinated
plasma
What is the most frequently employed automated method for glucose? - answers
Coupled enzyme system consisting of glucose oxidase and peroxidase.
, Contamination of the glucose reagent with catalase is a problem with which glucose
method? - answers Glucose oxidase
What is the hexokinase methodology for glucose determination based on? - answers
Reduced coenzyme read at 340 nm
What is the normal fasting blood glucose (FBS) reference range for adults? - answers
70-110 mg/dL
Prenatal patients with borderline blood glucose levels are further evaluated by which
test? - answers 3-hour glucose tolerance test (GTT)
Which test gives a 2-3 month picture of a diabetic's glucose levels? - answers
Hemoglobin A1C
What does the presence of urinary ketones MOST likely indicate? - answers Storage
lipids are being used as the primary energy source.
Which one of the following tests is used to differentiate between inappropriate
exogenous insulin administration and endogenous insulin production? - answers C-
peptide
The "port wine" color of some urines can be attributed to which of the following? -
answers Porphyrins
p-dimethylaminobenzaldehyde is a constituent of which reagent? - answers Ehrlich
Both porphobilinogen and urobilinogen form a red-colored compound with Ehrlich's
reagent. How can they be differentiated? - answers Urobilinogen is soluble in
chloroform
Prehepatic bilirubin has which of the following for a carrier? - answers Albumin
Direct-reacting bilirubin is also known as what substance? - answers Bilirubin
diglucaronide
An increase in indirect-reacting bilirubin is suggestive of which condition? - answers
Hemoglobin breakdown
Bilirubin is converted in the intestine to which of the following substances? - answers
Urobilinogen
Which of the following will interfere with the Evelyn Malloy method for bilirubin? -
answers Hemolysis
100% ACCURATE ANSWERS
Which of the four elements in proteins differentiated this class of substances from
carbohydrates and lipids? - answers Nitrogen
Which of the proteins is soluble in water? - answers Albumin
How are amino acids joined together to form a chainlike structure? - answers Peptide
bonding
Proteins may become denatured when subjected to mechanical agitation, heat, or
chemical treatment. What does denaturation of proteins refer to? - answers Alteration of
tertiary structure
Which aminoacidopathy is NOT caused by an enzyme deficiency? - answers Cystinuria
These aminoacidopathies are caused by an enzyme deficiency: - answers Maple syrup
disease, phenylketonuria, and alkaptonuria
How many immunoglobulin classes are currently recognized? - answers Five
Which reagent is employed in the serum protein determination? - answers Biuret
Why should hemolyzed serum not be used for total protein analysis? - answers
Hemoglobin is absorbed at the same wavelength as protein in the reagent
Which of the following is NOT true of the biuret reaction? - answers It is relatively free
from interference from lipids and hemoglobin
Which of the following is true of the biuret reaction? - answers Follows Beer's law for a
reasonable range of protein, depends on the presence of at least two peptide linkages,
and is insensitive to the low protein levels of urine.
Why is bromcresol purple the preferred indicator for albumin dye binding techniques? -
answers There is less interference from pigment(s)
What is the most anodic protein on electrophoresis at pH 8.6? - answers Albumin
The total protein is 7.1 g/dL. The serum protein results are:
Albumin 53.0% 3.8 g/dL
Alpha1 3.6% 0.3 g/dL
Alpha2 11.0% 0.8 g/dL
Beta 17.6% 1.2 g/dL
Gamma 14.8% 1.1 g/dL
,What is the A/G ratio? - answers 3.8/(.3+.8+1.2+1.1) = 1.1
A serum protein electrophoretic pattern displaying a beta gamma bridge or broad
gamma is seen in which disease state? - answers Active cirrhosis
If an electrophoretic pattern shows 30% albumin, 4%-10% alpha-globulin, 4%-10%
beta-globulin, and 45% gamma globulin, then what condition may exist in the patient? -
answers Monoclonal gammopathy
Glycoproteins and mucoproteins are usually bound to which substance? - answers
Carbohydrates
All of the following are glycoproteins: - answers Ceruloplasmin, follicle-stimulating
hormone (FSH), and fibrinogen
All of the following are glycoproteins EXCEPT: - answers Cryoglobulin
Which one of the following applies to cryoglobulins? - answers They are temperature-
sensitive proteins
Which of the following statements is true of albumin? - answers It is produced in the
liver and is water-soluble
Decreased alpha-fetoprotein values are associated with which condition? - answers
Down syndrome
Which one of the following proteins transports iron? - answers Transferrin
In acute renal failure, which nonprotein nitrogen rises the fastest? - answers Blood urea
nitrogen (BUN)
By the urease method, urea is enzymatically converted to which end product? - answers
Ammonia
Why can untreated urine be used for the determination of urea by the diacetyl
monoxomine method? - answers The method is not measuring ammonia
An elevated creatinine value is most likely to be accompanied by which of these values?
- answers Elevated BUN, 10X creatinine value
What is the classical method for creatinine reaction? - answers Jaffe
The measurement of creatinine is based on the formation of a yellow-red color and
which reagent? - answers Alkaline picrate
,The creatinine clearance test is based on which assumption? - answers Creatinine
passes into the ultrafiltrate
What is the most common clearance test used to measure the glomerular filtration rate?
(GFR) - answers Creatinine
A creatinine clearance test is performed. The 24-hour urine volume is 770 mL; serum
creatinine is 2.0 mg/dL and urine creatinine is 240 mg/dL. What is the clearance,
assuming average body surface area? - answers GFR = ([UCr x V]/SCr)/1440
64 mL/minute = [(240 x 770)/2]/1440
(1440 minutes in 24 hours)
Which disease state is associated with an elevation of serum uric acid? - answers Gout
Which reagent is employed in the alkaline oxidation of uric acid? - answers
Phosphotungstic acid
What does the uricase method for uric acid depend on? - answers UV absorption at 290
nm before/after treatment with uricase
Which one of the following analytes is used as a prognostic indicator for liver failure? -
answers Ammonia
Which disaccharide is composed of two glucose molecules? - answers Maltose
What term is used to describe the process of glycogen degradation? - answers
Glycogenolysis
What is the renal threshold for glucose? - answers 160-180 mg/dL
Which hormone is produced by the beta cells of the islets of Langerhans? - answers
Insulin
Which one of the following hormones is considered a glucocorticoid? - answers Cortisol
A 40-year-old male has a randome glucose of 470 mg/dL. This lab finding is consistent
with which one of the following clinical conditions? - answers Diabetes mellitus
Which one of the following diseases is associated with hypoglycemia? - answers von
Gierke's disease
What is the specimen of choice for glucose determinations? - answers Fluorinated
plasma
What is the most frequently employed automated method for glucose? - answers
Coupled enzyme system consisting of glucose oxidase and peroxidase.
, Contamination of the glucose reagent with catalase is a problem with which glucose
method? - answers Glucose oxidase
What is the hexokinase methodology for glucose determination based on? - answers
Reduced coenzyme read at 340 nm
What is the normal fasting blood glucose (FBS) reference range for adults? - answers
70-110 mg/dL
Prenatal patients with borderline blood glucose levels are further evaluated by which
test? - answers 3-hour glucose tolerance test (GTT)
Which test gives a 2-3 month picture of a diabetic's glucose levels? - answers
Hemoglobin A1C
What does the presence of urinary ketones MOST likely indicate? - answers Storage
lipids are being used as the primary energy source.
Which one of the following tests is used to differentiate between inappropriate
exogenous insulin administration and endogenous insulin production? - answers C-
peptide
The "port wine" color of some urines can be attributed to which of the following? -
answers Porphyrins
p-dimethylaminobenzaldehyde is a constituent of which reagent? - answers Ehrlich
Both porphobilinogen and urobilinogen form a red-colored compound with Ehrlich's
reagent. How can they be differentiated? - answers Urobilinogen is soluble in
chloroform
Prehepatic bilirubin has which of the following for a carrier? - answers Albumin
Direct-reacting bilirubin is also known as what substance? - answers Bilirubin
diglucaronide
An increase in indirect-reacting bilirubin is suggestive of which condition? - answers
Hemoglobin breakdown
Bilirubin is converted in the intestine to which of the following substances? - answers
Urobilinogen
Which of the following will interfere with the Evelyn Malloy method for bilirubin? -
answers Hemolysis
