“AQA GCSE (9–1) Biology Paper 2 – Full Exam Paper with
Detailed Answers and Official Mark Scheme | Updated
2025 Edition for Top Grades and Complete Revision Success”
Candidate surname Other names
Centre Number Candidate Number
Paper
⯁ ⯁
Biology
PAPER 2
Higher Tier
Marks
Instructions
•• Use black ink or ball‑point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer all questions.
Answer the questions in the spaces provided – there may be
more space than you need.
•• Calculators may be used.
•
Any diagrams may NOT be accurately drawn, unless otherwise indicated.
You must show all your working out with your answer clearly identified at the
end of your solution.
Information
•• The total mark for this paper is 100.
The marks for each question are shown in brackets
•
– use this as a guide as to how much time to spend on each question.
In questions marked with an asterisk (*), marks will be awarded for your ability to structure your answer
logically, showing how the points that you make are related or follow on from each other where appropriate.
Advice
• Try to answer every question.
••
Read each question carefully before you start to answer it.
Check your answers if you have time at the end.
Turn over
P69321A
©2022 Pearson Education Ltd.
,Q:1/1/1/1/1/
,
, Answer ALL questions. Write your answers in the spaces provided.
Some questions must be answered with a cross in a box . If you change your mind about an
answer, put a line through the box and then mark your new answer with a cross .
1 (a) Bloodworms in a pond indicate that the water is polluted.
(i) Which species also indicates that the water is polluted?
(1)
A fertiliser
B lichen
C stonefly
D sludgeworm
(ii) Bloodworms have a high level of haemoglobin in their blood.
Give a reason why this helps them survive in polluted water.
(1)
. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. .. ... .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. . . . ......................
. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. .. ... .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. . . . ......................
(iii) Carbon dioxide diffuses from the body of the bloodworm into the water.
Give two factors that affect the rate of diffusion.
(2)
1. . .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. . ... .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ..................
. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. .. ... .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... . . .. ... .. .. ... .. ... .. .. . ......................
2. . .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. . ... .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. . . .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ..................
. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. .. ... .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... . . .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. . ......................
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Detailed Answers and Official Mark Scheme | Updated
2025 Edition for Top Grades and Complete Revision Success”
Candidate surname Other names
Centre Number Candidate Number
Paper
⯁ ⯁
Biology
PAPER 2
Higher Tier
Marks
Instructions
•• Use black ink or ball‑point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer all questions.
Answer the questions in the spaces provided – there may be
more space than you need.
•• Calculators may be used.
•
Any diagrams may NOT be accurately drawn, unless otherwise indicated.
You must show all your working out with your answer clearly identified at the
end of your solution.
Information
•• The total mark for this paper is 100.
The marks for each question are shown in brackets
•
– use this as a guide as to how much time to spend on each question.
In questions marked with an asterisk (*), marks will be awarded for your ability to structure your answer
logically, showing how the points that you make are related or follow on from each other where appropriate.
Advice
• Try to answer every question.
••
Read each question carefully before you start to answer it.
Check your answers if you have time at the end.
Turn over
P69321A
©2022 Pearson Education Ltd.
,Q:1/1/1/1/1/
,
, Answer ALL questions. Write your answers in the spaces provided.
Some questions must be answered with a cross in a box . If you change your mind about an
answer, put a line through the box and then mark your new answer with a cross .
1 (a) Bloodworms in a pond indicate that the water is polluted.
(i) Which species also indicates that the water is polluted?
(1)
A fertiliser
B lichen
C stonefly
D sludgeworm
(ii) Bloodworms have a high level of haemoglobin in their blood.
Give a reason why this helps them survive in polluted water.
(1)
. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. .. ... .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. . . . ......................
. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. .. ... .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. . . . ......................
(iii) Carbon dioxide diffuses from the body of the bloodworm into the water.
Give two factors that affect the rate of diffusion.
(2)
1. . .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. . ... .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ..................
. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. .. ... .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... . . .. ... .. .. ... .. ... .. .. . ......................
2. . .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. . ... .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. . . .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ..................
. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. ... .. .. .. ... .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... . . .. ... .. .. ... .. .. ... .. .. .. .. ... .. .. ... .. ... .. .. ... .. .. ... .. .. ... .. ... .. .. . ......................
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