Edexcel A-Level Biology Paper 1 2025
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How can a paternity test show that an alleged father is not the
biological father? Ans: child has micro-satellites that are not from
mother or alleged father, so must be from a different person
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(biological father)
What are short tandem repeats? Ans: micro-satellite regions
commonly used in DNA identification
In Southern blotting, what is hybridisation? Ans: gene probes bind
with complementary strands
What is a gene probe? Ans: short DNA sequences,
complementary to specific sequences
What may be placed over a gel in Southern blotting? Ans: nylon
filter or nitrocellulose paper
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Hoe are DNA fragments extracted using Southern blotting? Ans:
alkaline buffer, absorbent filter placed over it
What dye is commonly used in gel electrophoresis? Ans: ethidium
bromide
What is the purpose of the second step of PCR? Ans: primers bind
to DNA strands
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What may be reasons somebody can't be vaccinated and so
relies on herd immunity? Ans: allergies, immune system diseases
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What is neutralisation in the immune response? Ans: antibodies
neutralise the effects of toxins
What happens when a macrophage engulfs a pathogen? Ans:
combines pathogen antigens with MHC proteins
What substance is broken down in cellular respiration? Ans:
respiratory susbstrate
What is the main respiratory substrate? Ans: glucose
What catalyses the hydrolysis of ATP? Ans: ATPase
What is produced by the hydrolysis of ATP? Ans: ADP + phosphate
, 3
What causes rigor mortis? Ans: ATP production stops
What are the two main phases of respiration? Ans: glycolysis,
Krebs cycle
What part of respiration requires oxygen? Ans: Krebs cycle
Where does glycolysis take place? Ans: cytoplasm
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Where does the link reaction, electron transport chain, and Krebs
cycle happen? Ans: mitochondria
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What are stalked particles of a mitochondrion? Ans: site of ATP
production on cristae
What cell types have large amounts of mitochondria, and why?
Ans: active cells, high energy demand
What is a molecule that receives hydrogen to become reduced?
Ans: hydrogen acceptor
What is NAD? Ans: conenzyme hydrogen acceptor in respiration
What is NADH? Ans: reduced NAD
What is NAD+? Ans: oxidised NAD
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Name two hydrogen acceptors in respiration and their reduced
molecules Ans: NAD -> NADH, FAD -> FADH2
How can respiration of a whole organism be measured? Ans:
respirometer
What is used to absorb the carbon dioxide produced by
organisms when respiration is measured? Ans: soda lime
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What are three ways to investigate respiration in cell fragments?
Ans: centrifuge to separate mitochondria, electron microscope,
separate stalked particles
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How do mitochondria separated from their cells prove respiration
when supplied with oxygen and glucose? Ans: ATP is produced
What information can an electron microscope give about
mitochondria? Ans: stalked particles provide a large SA
What is produced by glycolysis? Ans: pyruvate
What are the four main stages of glycolysis? Ans: 6C glucose ->
6C phosphorylated sugar -> 2x3C sugar -> 2x3C pyruvate
What happens between 6C glucose -> 6C phosphorylated sugar?
Ans: ATP -> ADP