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MICROBIOLOGY LAB FINAL – (GCSU BACHOON)
2025/2026 EXAM CURRENTLY TESTING QUESTIONS
AND CORRECT DETAILED ANSWERS (VERIFIED)
FOR GUARANTEED PASS/TOP-RATED A+.
MICROBIOLOGY LAB
Ace your Microbiology Lab Final (Bachoon-GCSU) with this
targeted guide, designed to master essential laboratory
techniques, staining procedures, and microbial identification.
This resource delivers practical, scenario-based questions
covering aseptic methods, biochemical testing, and safety
protocols.
According to health care experts, infectious diseases
caused by ______________ are responsible for more deaths
worldwide than any other single cause. ...... ANSWER .......
microbes
Adequate precautions are necessary to prevent unwanted
_________________ from getting in or on experimental tubes
or plates. ...... ANSWER ....... contaminants
What is the first step of aseptic technique? ...... ANSWER
....... Flame the inoculating loop
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What is the second step of aseptic technique? ......
ANSWER ....... mix up organisms in culture
What is the third step of aseptic technique? ...... ANSWER
....... flame the mouth of the tube
What is the fourth step of aseptic technique? ...... ANSWER
....... remove organisms from tube using the inoculating
loop.
What is the fifth step of aseptic technique? ...... ANSWER
....... remove loop from culture and flame the mouth of
the tube and close the tube
What is the 6th step of aseptic technique? ...... ANSWER
....... transfer the loopful of bacteria to a test tube or
Petri plate
What is the seventh step of aseptic technique? ......
ANSWER ....... After transferring, flame the loop
What bacteria did we use in the Aseptic Technique lab
(Exercise 2)? ...... ANSWER ....... E. coli
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used in carrying the microscope; houses the light source
...... ANSWER ....... Base
provides illumination to specimen ...... ANSWER .......
light source
along with the base, used as a carrying handle for the
microscope ...... ANSWER ....... arm
platform which the specimen rests on ...... ANSWER .......
stage
controls the distance between the specimen and the stage
...... ANSWER ....... course and fine adjustment knobs
is used with scan and low power objective lenses ......
ANSWER ....... coarse focus
is used with high dry and oil immersion objective lenses
...... ANSWER ....... fine focus
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allow the movement of the stage from left to right and
forward to backward ...... ANSWER ....... mechanical
stage adjusters
collects and concentrates light emitted from the light
sources into a tight beam before passing it through the
specimen ...... ANSWER ....... condenser
located within the condenser; tightens or widens the beam
of light emitted from the condenser ...... ANSWER .......
iris diaphragm
controls the amount of light emitted from the source bulb
...... ANSWER ....... light intensity adjuster
serve to magnify the specimen in part ...... ANSWER .......
objective lenses
4x magnification ...... ANSWER ....... scanning power
10x magnification ...... ANSWER ....... low power
MICROBIOLOGY LAB FINAL – (GCSU BACHOON)
2025/2026 EXAM CURRENTLY TESTING QUESTIONS
AND CORRECT DETAILED ANSWERS (VERIFIED)
FOR GUARANTEED PASS/TOP-RATED A+.
MICROBIOLOGY LAB
Ace your Microbiology Lab Final (Bachoon-GCSU) with this
targeted guide, designed to master essential laboratory
techniques, staining procedures, and microbial identification.
This resource delivers practical, scenario-based questions
covering aseptic methods, biochemical testing, and safety
protocols.
According to health care experts, infectious diseases
caused by ______________ are responsible for more deaths
worldwide than any other single cause. ...... ANSWER .......
microbes
Adequate precautions are necessary to prevent unwanted
_________________ from getting in or on experimental tubes
or plates. ...... ANSWER ....... contaminants
What is the first step of aseptic technique? ...... ANSWER
....... Flame the inoculating loop
, Page 2 of 49
What is the second step of aseptic technique? ......
ANSWER ....... mix up organisms in culture
What is the third step of aseptic technique? ...... ANSWER
....... flame the mouth of the tube
What is the fourth step of aseptic technique? ...... ANSWER
....... remove organisms from tube using the inoculating
loop.
What is the fifth step of aseptic technique? ...... ANSWER
....... remove loop from culture and flame the mouth of
the tube and close the tube
What is the 6th step of aseptic technique? ...... ANSWER
....... transfer the loopful of bacteria to a test tube or
Petri plate
What is the seventh step of aseptic technique? ......
ANSWER ....... After transferring, flame the loop
What bacteria did we use in the Aseptic Technique lab
(Exercise 2)? ...... ANSWER ....... E. coli
, Page 3 of 49
used in carrying the microscope; houses the light source
...... ANSWER ....... Base
provides illumination to specimen ...... ANSWER .......
light source
along with the base, used as a carrying handle for the
microscope ...... ANSWER ....... arm
platform which the specimen rests on ...... ANSWER .......
stage
controls the distance between the specimen and the stage
...... ANSWER ....... course and fine adjustment knobs
is used with scan and low power objective lenses ......
ANSWER ....... coarse focus
is used with high dry and oil immersion objective lenses
...... ANSWER ....... fine focus
, Page 4 of 49
allow the movement of the stage from left to right and
forward to backward ...... ANSWER ....... mechanical
stage adjusters
collects and concentrates light emitted from the light
sources into a tight beam before passing it through the
specimen ...... ANSWER ....... condenser
located within the condenser; tightens or widens the beam
of light emitted from the condenser ...... ANSWER .......
iris diaphragm
controls the amount of light emitted from the source bulb
...... ANSWER ....... light intensity adjuster
serve to magnify the specimen in part ...... ANSWER .......
objective lenses
4x magnification ...... ANSWER ....... scanning power
10x magnification ...... ANSWER ....... low power
