ADVANCED BIOCHEM ACS EXAM
QUESTIONS & ANSWERS
Coenzymes required by the pyruvate dehydrogenase multienzyme complex are -
ANSWERThiamine pyrophosphate, lipoic acid, Fad, CoA, & NAD+
A kinase is an enzyme that... - ANSWERtransfers a phosphoryl group from ATP
Which of these contains a thioester bond: - ANSWERsuccinyl CoA
In setting up an assay for measuring the total amount of an enzyme present in an
unknown sample, the substrate concentration should preferably be: - ANSWER10 fold
higher than Km
Dialysis would be most useful for the separation of: - ANSWERA ammonium sulfate
from an enzyme
An inhibitor that increases Km and does not alter the V max value is - ANSWERA
competitive inhibitor
Which amino acid side chain would not be expected would not be expected to
participate in covalent enzyme catalysis - ANSWERLeu
Which statement is correct concerning the T(deoxy) and R(oxy) states of tetrameric
hemoglobin - ANSWERIn the T to R transition, the central cavity of the tetramer
decreases in size, preventing the binding of the allosteric modulator 2,3,-
biophosphylglycerate
When the energy needs of the cell are satisfied, acetyl-CoA accumulates and serves as
a positive allosteric effector for - ANSWERpyruvate carboxylase
To determine the Km value for an enzyme reaction, which parameter is normally varied,
while keeping the others constant - ANSWERsubstrate concentration
In a yeast cell growing aerobically on glucose, which would provide most of the energy
for growth? - ANSWERglycolysis to TCA cycle and oxidative phosphorylation
Cleavage of disulfide bonds in globular proteins may be brought about by -
ANSWERReaction with excess B-mercaptoethanol at pH 8 in 6 M urea
The alpha helical secondary structure found in protein - ANSWERis disrupted by
proline, amine H and carbonyl O
QUESTIONS & ANSWERS
Coenzymes required by the pyruvate dehydrogenase multienzyme complex are -
ANSWERThiamine pyrophosphate, lipoic acid, Fad, CoA, & NAD+
A kinase is an enzyme that... - ANSWERtransfers a phosphoryl group from ATP
Which of these contains a thioester bond: - ANSWERsuccinyl CoA
In setting up an assay for measuring the total amount of an enzyme present in an
unknown sample, the substrate concentration should preferably be: - ANSWER10 fold
higher than Km
Dialysis would be most useful for the separation of: - ANSWERA ammonium sulfate
from an enzyme
An inhibitor that increases Km and does not alter the V max value is - ANSWERA
competitive inhibitor
Which amino acid side chain would not be expected would not be expected to
participate in covalent enzyme catalysis - ANSWERLeu
Which statement is correct concerning the T(deoxy) and R(oxy) states of tetrameric
hemoglobin - ANSWERIn the T to R transition, the central cavity of the tetramer
decreases in size, preventing the binding of the allosteric modulator 2,3,-
biophosphylglycerate
When the energy needs of the cell are satisfied, acetyl-CoA accumulates and serves as
a positive allosteric effector for - ANSWERpyruvate carboxylase
To determine the Km value for an enzyme reaction, which parameter is normally varied,
while keeping the others constant - ANSWERsubstrate concentration
In a yeast cell growing aerobically on glucose, which would provide most of the energy
for growth? - ANSWERglycolysis to TCA cycle and oxidative phosphorylation
Cleavage of disulfide bonds in globular proteins may be brought about by -
ANSWERReaction with excess B-mercaptoethanol at pH 8 in 6 M urea
The alpha helical secondary structure found in protein - ANSWERis disrupted by
proline, amine H and carbonyl O
