ASCP BOARD OF CERTIFICATION MLT
EXAM REVIEW QUESTIONS WITH
CORRECT ANSWERS 2025-2026
Nephelometry is based on the principle of: CORRECT ANSWER Turbidity resulting from specific
antigen- coated latex particles agglutinated by corresponding antibody
Which of the following can cause hemolysis and perhaps result in recollection of the specimen?
CORRECT ANSWER Pushing the plunger of a syringe when transferring blood into vacuum tubes.
A manual white blood cell count waskperformed by the hematology technologist. The cell counts for both
sides were 99 and 164, respectively. All nine large squares were counted on each side. The dilution for thi
s kit was pre-measured at 1:100. What should the technologist report as the white cell count? -
CORRECT ANSWER -Cannotkreport
When comparing the white cell counts from one side of the hemacytometer to the other, there is not goo
d duplication. Cell counts from opposite sides of the hemacytometer (within the same patient sample) th
at are greater than 10% of one another are considered discrepant and should not be used to calculate rep
ortable results. This particular count should not be used to report patient results.
The RBC inclusions shown in the photograph represent which of the following? CORRECT
ANSWER Howell-Jolly bodies
Howell-
Jolly bodies are usually single, round, and tend to be positioned at the periphery of the red blood cell. The
se inclusions are rarely found on a routine peripheral blood smear, as they are usually removed by a funct
ioning spleen. Howell-Jolly bodies appear in megaloblastic anemias and after splenectomy.
Which of the following tests would be employed in order to detect neural tube defects?
CORRECT ANSWER Alpha fetoprotein
Alpha-
fetoprotein is a protein measured in pregnant women, using maternal blood or amniotic fluid, as a screen
ingktest for a subset developmental abnormalities: it is principally increased in open neural tube defects a
nd decreased in Down syndrome.
,A patient has the following serum results:
Na = 130 mEq/LK = 4.9 mEq/LCl = 107 mEq/LGlucose = 300 mg/dLBUN = 18 mg/dLCreatinine = 1.1 mg/dL
Which osmolality result is consistent with these results?. CORRECT ANSWER 281 mosmol/kg
Osmolality = 2 x Na + [Glucose (mg/dL) / 20] + [BUN (mg/dL)/ 3]
A liquid ion-exchange membrane electrode using the antibiotic valinomycin is MOST selective for:
CORRECT ANSWER Potassium
The valinomycin membrane electrode is most selective for potassium as opposed to sodium, glucose, or c
hloride which utilize different membranes for their respective electrodes. Valinomycin, which is neutral in
charge, has been incorporated into organic liquid membranes resulting in a sensor with a very high select
ivity for potassium.
What should safety management start with in order to ensure clarity and consistency?
CORRECT ANSWER A written safety policy
Which of the following statements aboutkhigh-frequency antigens is correct? CORRECT ANSWER
High- frequency antigens are common, but it is difficult to identify their corresponding antibodies.
An adult's plasma cortisol of 22 µg/dL at 8:00 a.m. and 10 µg/dL at 4:00 p.m. would be:
CORRECT ANSWER Found in a healthy individual
Normal levels: 5-23 µg/dL at 8:00 a.m.
3-16 µg/dL at 4:00 p.m.
<50% of 8:00 a.m. values at 8:00 p.m.
A peripheral smear demonstrates a population of red cells that are mostly 5 -
5.5 µm in diameter. The CBC data shows RDW of 13% (reference range 11.5-
14.5%). Which of the following morphologies is consistent with these findings? CORRECT
ANSWER Microcytosis, low variation of cell volume
, Select the primary reagent components used in the Jaffe reaction for creatinine. CORRECT
ANSWER Saturated picric acid and sodium hydroxide
Which of the following are markers currently used to differentiate heart failure from lung disease?
CORRECT ANSWER BNP and NT-proBNP
Which of the following is a selective medium for Campylobacter jejuni? -kCORRECT ANSWER -Skirrow agar
Which Lewis antigen(s) would be detected when phenotyping the red cells of an adult who has the Le, Se,
and H genes? -kCORRECT ANSWER -Leb
The Se gene codes for the enzyme, a-2-L-fucosyltransferase, which adds L-
fucose to the type 1 precursor H (type 1). The Le gene codes for another L-
fucose which forms the Leb antigen.
Illustrated in the top photograph are acid-fast bacilli as they may appear in acid-
fast stains prepared from a direct mount of infected tissue. These acid fast bacilli are thin, slightly curved,
red-
pigmented and beaded. The acid fast bacilli, as illustrated in the bottom photograph, aggregate in serpent
ine chords in stained mounts prepared from colonies growing on agar. From these observations, select th
e presumptive identification of this mycobacterium. CORRECT ANSWER Mycobacterium
tuberculosis
The predominant cells seen on the CSF smear in this illustration are indicative of: CORRECT
ANSWER Bacterial meningitis
Aknewborn presents with neonatal alloimmune thrombocytopenia. What is the most likely cause of thiskc
ondition? -kCORRECT ANSWER -Maternal antibodies to neonatal plateletkantigens
Aklipid panel consists of which of the following tests? CORRECT ANSWER
Cholesterol, Lipoprotein, HDL, Triglycerides
Antibodies of which of the following immunoglobulin subclasses is predominantly associated with the sec
EXAM REVIEW QUESTIONS WITH
CORRECT ANSWERS 2025-2026
Nephelometry is based on the principle of: CORRECT ANSWER Turbidity resulting from specific
antigen- coated latex particles agglutinated by corresponding antibody
Which of the following can cause hemolysis and perhaps result in recollection of the specimen?
CORRECT ANSWER Pushing the plunger of a syringe when transferring blood into vacuum tubes.
A manual white blood cell count waskperformed by the hematology technologist. The cell counts for both
sides were 99 and 164, respectively. All nine large squares were counted on each side. The dilution for thi
s kit was pre-measured at 1:100. What should the technologist report as the white cell count? -
CORRECT ANSWER -Cannotkreport
When comparing the white cell counts from one side of the hemacytometer to the other, there is not goo
d duplication. Cell counts from opposite sides of the hemacytometer (within the same patient sample) th
at are greater than 10% of one another are considered discrepant and should not be used to calculate rep
ortable results. This particular count should not be used to report patient results.
The RBC inclusions shown in the photograph represent which of the following? CORRECT
ANSWER Howell-Jolly bodies
Howell-
Jolly bodies are usually single, round, and tend to be positioned at the periphery of the red blood cell. The
se inclusions are rarely found on a routine peripheral blood smear, as they are usually removed by a funct
ioning spleen. Howell-Jolly bodies appear in megaloblastic anemias and after splenectomy.
Which of the following tests would be employed in order to detect neural tube defects?
CORRECT ANSWER Alpha fetoprotein
Alpha-
fetoprotein is a protein measured in pregnant women, using maternal blood or amniotic fluid, as a screen
ingktest for a subset developmental abnormalities: it is principally increased in open neural tube defects a
nd decreased in Down syndrome.
,A patient has the following serum results:
Na = 130 mEq/LK = 4.9 mEq/LCl = 107 mEq/LGlucose = 300 mg/dLBUN = 18 mg/dLCreatinine = 1.1 mg/dL
Which osmolality result is consistent with these results?. CORRECT ANSWER 281 mosmol/kg
Osmolality = 2 x Na + [Glucose (mg/dL) / 20] + [BUN (mg/dL)/ 3]
A liquid ion-exchange membrane electrode using the antibiotic valinomycin is MOST selective for:
CORRECT ANSWER Potassium
The valinomycin membrane electrode is most selective for potassium as opposed to sodium, glucose, or c
hloride which utilize different membranes for their respective electrodes. Valinomycin, which is neutral in
charge, has been incorporated into organic liquid membranes resulting in a sensor with a very high select
ivity for potassium.
What should safety management start with in order to ensure clarity and consistency?
CORRECT ANSWER A written safety policy
Which of the following statements aboutkhigh-frequency antigens is correct? CORRECT ANSWER
High- frequency antigens are common, but it is difficult to identify their corresponding antibodies.
An adult's plasma cortisol of 22 µg/dL at 8:00 a.m. and 10 µg/dL at 4:00 p.m. would be:
CORRECT ANSWER Found in a healthy individual
Normal levels: 5-23 µg/dL at 8:00 a.m.
3-16 µg/dL at 4:00 p.m.
<50% of 8:00 a.m. values at 8:00 p.m.
A peripheral smear demonstrates a population of red cells that are mostly 5 -
5.5 µm in diameter. The CBC data shows RDW of 13% (reference range 11.5-
14.5%). Which of the following morphologies is consistent with these findings? CORRECT
ANSWER Microcytosis, low variation of cell volume
, Select the primary reagent components used in the Jaffe reaction for creatinine. CORRECT
ANSWER Saturated picric acid and sodium hydroxide
Which of the following are markers currently used to differentiate heart failure from lung disease?
CORRECT ANSWER BNP and NT-proBNP
Which of the following is a selective medium for Campylobacter jejuni? -kCORRECT ANSWER -Skirrow agar
Which Lewis antigen(s) would be detected when phenotyping the red cells of an adult who has the Le, Se,
and H genes? -kCORRECT ANSWER -Leb
The Se gene codes for the enzyme, a-2-L-fucosyltransferase, which adds L-
fucose to the type 1 precursor H (type 1). The Le gene codes for another L-
fucose which forms the Leb antigen.
Illustrated in the top photograph are acid-fast bacilli as they may appear in acid-
fast stains prepared from a direct mount of infected tissue. These acid fast bacilli are thin, slightly curved,
red-
pigmented and beaded. The acid fast bacilli, as illustrated in the bottom photograph, aggregate in serpent
ine chords in stained mounts prepared from colonies growing on agar. From these observations, select th
e presumptive identification of this mycobacterium. CORRECT ANSWER Mycobacterium
tuberculosis
The predominant cells seen on the CSF smear in this illustration are indicative of: CORRECT
ANSWER Bacterial meningitis
Aknewborn presents with neonatal alloimmune thrombocytopenia. What is the most likely cause of thiskc
ondition? -kCORRECT ANSWER -Maternal antibodies to neonatal plateletkantigens
Aklipid panel consists of which of the following tests? CORRECT ANSWER
Cholesterol, Lipoprotein, HDL, Triglycerides
Antibodies of which of the following immunoglobulin subclasses is predominantly associated with the sec
