AP Biology Chapter 25 – 2025/2026 | 40+ Practice Questions with Correct Answers | Macroevolution, Origin of Life, Mass Extinctions

This document presents over 40 expertly crafted Q&A-style practice questions with 100% correct answers, based on Chapter 25 of the AP Biology curriculum, fully aligned with the 2025/2026 College Board standards. It focuses on the history of life on Earth, providing an in-depth review of macroevolutionary processes, major biological transitions, and the geological and molecular evidence supporting the origin and evolution of life. Core concepts include: – Macroevolutionary events: such as the appearance of photosynthesis, first vertebrates, and mass extinctions – The origin of life on Earth: conditions of early Earth, formation of organic molecules, protobionts, RNA world hypothesis – Fossil dating techniques: including carbon-14 dating, sedimentary rock analysis, and magnetic reversal evidence – Major evolutionary transitions: prokaryotes, endosymbiosis, and tetrapod evolution – Continental drift, adaptive radiation, and extinction events: including the Permian and Cretaceous mass extinctions – Key evo-devo concepts: like heterochrony, exaptation, and Hox gene function The document is ideal for consolidating understanding, enhancing recall, and practicing application of high-yield concepts in a self-assessment format. Best suited for: – AP Biology students preparing for exams – IB Biology students studying evolution and geological history – Introductory college biology students – Tutors and educators looking for focused practice content on evolutionary biology – Students preparing unit tests or cumulative exams on macroevolutionary topics Its structure allows for focused study, making complex concepts accessible while encouraging mastery of evolutionary timelines, molecular evolution, and developmental biology integrations. Keywords: AP Biology, Chapter 25, macroevolution, origin of life, RNA world, adaptive radiation, mass extinction, endosymbiosis, fossil dating, continental drift, evo-devo, Hox genes, heterochrony, exaptation, early Earth, evolutionary biology, College Board