MCB 2000 EXAM 5 UCONN STUDY GUIDE EXAM AND ACTUAL ANSWERS EDITION.

MCB 2000 EXAM 5 UCONN STUDY
GUIDE EXAM AND ACTUAL ANSWERS
2025-2026 EDITION.
What is gluconeogenesis? - Answer synthesis of glucose from non-carbohydrate precursors



What is pyruvate? - Answer - The end product of glycolysis

-The first step in cellular respiration

- Stands in the junction between anaerobic and aerobic pathways



Is gluconeogenesis anabolic or catabolic? - Answer anabolic



What replaces the three irreversible steps of glycolysis in gluconeogenesis? - Answer
Pyruvate carboxylase, Phosphoenolpyruvate carboxykinase, fructose 1-6 bisphosphotase and
glucose 6 phosphotase



Step 1 of gluconeogenesis - Answer pyruvate is carboxylated by adding CO2 from
bicarbonate requiring a biotin cofactor ( uses ATP)



what enzyme is responsible for step one of gluconeogenesis? - Answer Pyruvate carboxylase



Step 2 of gluconeogenesis - Answer phosphoenolpyruvate (PEP) carboxykinase converts
oxaloacetate to PEP. Requires GTP as an energy source and CO2 is released along with GDP



what enzyme is responsible for step 2 of gluconeogenesis? - Answer phosphoenolpyruvate
(PEP) carboxykinase



Step 3 of gluconeogenesis - Answer (2) Phosphoenolpyruvate + 2H2O --> (2) 2-
phosphoglycerate



what enzyme is responsible for step 3 of gluconeogenesis? - Answer Enolase



Step 4 of gluconeogenesis - Answer 2-phosphoglycerate to 3-phosphoglycerate

, Step 5 of gluconeogenesis - Answer 3-phosphoglycerate --> 1,3 bisphosphoglycerate (uses
ATP)



what enzyme is responsible for step 5 of gluconeogenesis? - Answer phosphoglycerate
kinase



Step 6 of gluconeogenesis - Answer 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate



what enzyme is responsible for step 6 of gluconeogenesis? - Answer glyceraldehyde 3
phosphate dehydrogenase (uses NADH)



Step 7 of gluconeogenesis - Answer one GA3P is turned into a DHAP (now we have 1 GA3P
and 1 DHAP)



what enzyme is responsible for step 7 of gluconeogenesis? - Answer triose phosphate
isomerase



Step 8 of gluconeogenesis - Answer 1 GA3P and 1 DHAP to fructose 1-6 bisphosphate



what enzyme is responsible for step 8 of gluconeogenesis? - Answer aldolase



step 9 of gluconeogenesis - Answer fructose 1-6 bisphosphate to fructose 6 phosphate (uses
1 H2O)



what enzyme is responsible for step 9 of gluconeogenesis? - Answer fructose 1-6
bisphosphatase



Step 10 of gluconeogenesis - Answer Fructose 6- phosphate -->Glucose 6-phosphate



what enzyme is responsible for step 10 of gluconeogenesis? - Answer phosphoglucose
isomerase AKA phosphohexose isomerase



Step 11 of gluconeogenesis - Answer glucose 6 phosphate to glucose