1|Page
MMSC 407 Clinical Chemistry Final Exam||
QUESTIONS AND CORRECT DETAILED ANSWERS
(VERIFIED ANSWERS) |ALREADY GRADED
A+||NEWEST EXAM!!!
Must ___ urine to prevent the precipitation of calcium
salts. - ANSWER-acidify
What is the most common method for detecting calcium? -
ANSWER-spectrophotometer
Red complex at 540nm
What is added to eliminate magnesium interference? -
ANSWER-8-Hydroxyquinolone
Phosphate acts as a principle ____ of the urinary system.
- ANSWER-buffer
T/F most of plasma phosphate is bound to protein. -
ANSWER-false
Most plasma phosphate is free
10% bound to protein
35% bound to Ca or Mg
,2|Page
Physiologic roles of phosphorus - ANSWER-energy
metabolism
Mineralization of bone
Blood buffer
Hyperphosphatemia - ANSWER-greater than 4.5mg/dl
Diseases associated with hyperphosphatemia - ANSWER-
primary hypoparathyroidism
Renal failure
Vitamin D toxicity
Hypophosphatemia - ANSWER-less than 2.4mg/dl
Conditions associated with hypophosphatemia -
ANSWER-renal transport failure-fanconis syndrome
Hyperparathyroidism
Specimens for phosphorus - ANSWER-serum fasting
Urine preserved in 6 M hcl
,3|Page
Inorganic phosphorus will ____ over time as organic
phosphorus is converted. - ANSWER-increase
Fasting serum/plasma phosphorus values for adults -
ANSWER-2.4-4.5mg/dl
An amino acid is a compound having both a: - ANSWER-
carboxyl and amino group
Ph dependent properties of amino acids - ANSWER-
zwitterion
Sequence of amino acids can be determined using the
____ - ANSWER-enzyme carboxypeptidase
Levels of protein structure - ANSWER-primary, secondary,
tertiary, quaternary
The biuret reagent is used to test the amount of___ -
ANSWER-proteins
The reference range for a healthy total protein? -
ANSWER-6.4-8.3 g/dl
, 4|Page
What is responsible for the overall shape or confirmation
of a protein? - ANSWER-H+ bonding, dispersion forces,
ionic interactions
Tertiary structure of protein - ANSWER-rises from weaker
attractive forces between hydrophobic parts of the same
chain
Tertiary structure of protein can be seen as: - ANSWER-
braids and globs
Ex-hemoglobin
Hemoglobin has __ polypeptide chains and has a ___
structure - ANSWER-4; quaternary
Myoglobin has a single chain of___ - ANSWER-amino
acids
Denaturation irreversible example: - ANSWER-making
eggs
We need to take in ___ grams of protein daily. - ANSWER-
70-80
MMSC 407 Clinical Chemistry Final Exam||
QUESTIONS AND CORRECT DETAILED ANSWERS
(VERIFIED ANSWERS) |ALREADY GRADED
A+||NEWEST EXAM!!!
Must ___ urine to prevent the precipitation of calcium
salts. - ANSWER-acidify
What is the most common method for detecting calcium? -
ANSWER-spectrophotometer
Red complex at 540nm
What is added to eliminate magnesium interference? -
ANSWER-8-Hydroxyquinolone
Phosphate acts as a principle ____ of the urinary system.
- ANSWER-buffer
T/F most of plasma phosphate is bound to protein. -
ANSWER-false
Most plasma phosphate is free
10% bound to protein
35% bound to Ca or Mg
,2|Page
Physiologic roles of phosphorus - ANSWER-energy
metabolism
Mineralization of bone
Blood buffer
Hyperphosphatemia - ANSWER-greater than 4.5mg/dl
Diseases associated with hyperphosphatemia - ANSWER-
primary hypoparathyroidism
Renal failure
Vitamin D toxicity
Hypophosphatemia - ANSWER-less than 2.4mg/dl
Conditions associated with hypophosphatemia -
ANSWER-renal transport failure-fanconis syndrome
Hyperparathyroidism
Specimens for phosphorus - ANSWER-serum fasting
Urine preserved in 6 M hcl
,3|Page
Inorganic phosphorus will ____ over time as organic
phosphorus is converted. - ANSWER-increase
Fasting serum/plasma phosphorus values for adults -
ANSWER-2.4-4.5mg/dl
An amino acid is a compound having both a: - ANSWER-
carboxyl and amino group
Ph dependent properties of amino acids - ANSWER-
zwitterion
Sequence of amino acids can be determined using the
____ - ANSWER-enzyme carboxypeptidase
Levels of protein structure - ANSWER-primary, secondary,
tertiary, quaternary
The biuret reagent is used to test the amount of___ -
ANSWER-proteins
The reference range for a healthy total protein? -
ANSWER-6.4-8.3 g/dl
, 4|Page
What is responsible for the overall shape or confirmation
of a protein? - ANSWER-H+ bonding, dispersion forces,
ionic interactions
Tertiary structure of protein - ANSWER-rises from weaker
attractive forces between hydrophobic parts of the same
chain
Tertiary structure of protein can be seen as: - ANSWER-
braids and globs
Ex-hemoglobin
Hemoglobin has __ polypeptide chains and has a ___
structure - ANSWER-4; quaternary
Myoglobin has a single chain of___ - ANSWER-amino
acids
Denaturation irreversible example: - ANSWER-making
eggs
We need to take in ___ grams of protein daily. - ANSWER-
70-80
