During the agarose gel electrophoresis, if the size (length) of DNA molecules are
longer, the mobility will be _____, so the distance will be _____.
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The mobility will be slower, so the distance will be smaller.
PCR (polymerase chain reaction) consists of _____ steps that constitute one cycle, and
is repeated for many cycles. At the end of each cycle, there is up to _____ as much of
the target sequence or amplicon as was present at the beginning of the cycle.
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, Three; Twice
Review differences and similarities of Sanger sequencing and PCR.
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- Similarity: polymerase, dNTP, templates, primer.
- Difference: One primer for Sanger sequencing, fluorescently labeled
ddNTP for Sanger sequencing.
What is annealing?
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The temperature is lowered and two short, single-stranded primers are
able to bind to their complementary targets in the sample.
Lab #8 CRISPR editing (restriction digestion)
a. Upon digestion, one of the individuals digests at this cut site while the other does
not. Which result, if any, indicates successful mutagenesis? Explain.
Give this one a try later!
a. If you design your sgRNA such that it includes a restriction site, mutation
may result in disruption of the site.
longer, the mobility will be _____, so the distance will be _____.
Give this one a try later!
The mobility will be slower, so the distance will be smaller.
PCR (polymerase chain reaction) consists of _____ steps that constitute one cycle, and
is repeated for many cycles. At the end of each cycle, there is up to _____ as much of
the target sequence or amplicon as was present at the beginning of the cycle.
Give this one a try later!
, Three; Twice
Review differences and similarities of Sanger sequencing and PCR.
Give this one a try later!
- Similarity: polymerase, dNTP, templates, primer.
- Difference: One primer for Sanger sequencing, fluorescently labeled
ddNTP for Sanger sequencing.
What is annealing?
Give this one a try later!
The temperature is lowered and two short, single-stranded primers are
able to bind to their complementary targets in the sample.
Lab #8 CRISPR editing (restriction digestion)
a. Upon digestion, one of the individuals digests at this cut site while the other does
not. Which result, if any, indicates successful mutagenesis? Explain.
Give this one a try later!
a. If you design your sgRNA such that it includes a restriction site, mutation
may result in disruption of the site.
