ALAT CERTIFICATION FINAL EXAM
LATEST 2025-2026 QUESTIONS AND
ANSWERS 200 REAL EXAM QUESTIONS
AND ANSWERS (VERIFIED ANSWERS)
Which of the following species is covered by the Animal Welfare Act?
-Birds bred specifically for research
-Zebrafish
-Mice
-Hamsters - ANSWER- Hamsters
What types of euthanasia methods are listed in the AVMA Guidelines for the
Euthanasia of Animals?
-Suitable, conditionally suitable, and unsuitable methods
-Adequate, acceptable, and inadequate methods
-Acceptable, conditionally acceptable, and unacceptable methods
-Approved, tentative, and unapproved methods - ANSWER- Acceptable,
conditionally acceptable, and unacceptable methods
The PHS Policy covers all live vertebrates used or intended for use in research,
training, experimental purposes and biological testing.
-False
-True - ANSWER- True
,Which document on animal research is reviewed and approved by the Institutional
Animal Care and Use Committee?
-Research proposal
-Grant
-Animal use protocol
-Research data report - ANSWER- Animal Use Protocol
What federal agency is responsible for enforcing and administering the Animal
Welfare Regulations?
-Animal and Plant Health Inspection Service
-Food and Drug Administration
-Office of Laboratory Animal Welfare
-AAALAC International - ANSWER- Animal and Plant Health Inspection Service
What is NOT a requirement of the AWA/AWR a researcher must comply with, if
they house covered species?
-Appoint an unaffiliated member to the IACUC.
-Submit an annual report including the number of covered species used.
-Provide dogs with exercise.
-Meet or exceed sanitization standards for both cages and facility. - ANSWER-
Appoint an unaffiliated member to the IACUC.
7. According to the Animal Welfare Regulations, to which official does the
IACUC report?
-The Institutional Official (IO) or Chief Executive Officer (CEO)
-The Chair of the Office of Laboratory Animal Welfare or the Area Director
-The Director of the USDA Animal & Plant Health Inspection Service or Chief
Inspecting Officer
-The Attending Veterinarian or Facility Director - ANSWER- The Institutional
Official (IO) or Chief Executive Officer (CEO)
Which agency is responsible for enforcing the Public Health Service Policy on
Humane Care and Use of Laboratory Animals (PHS Policy)?
-Food and Drug Administration
-Animal and Plant Health Inspection Service
, -Office of Laboratory Animal Welfare
-National Research Council - ANSWER- Office of Laboratory Animal Welfare
What is NOT stated as a requirement for compliance with the US Principles for the
Utilization and Care of Vertebrate Animals Used in Testing, Research and
Training?
-Transportation, use and care of animals should be in accordance with the Animal
Welfare Act.
-Principal investigators should determine where exceptions to these principles are
required and may ignore them if they decide it is a scientific necessity.
-Procedures that cause more than momentary or slight pain must be performed
with appropriate sedation, analgesia and anesthesia.
-Animals that suffer unrelieved pain and distress should be painlessly killed at the
end of the procedure. - ANSWER- Principal investigators should determine where
exceptions to these principles are required and may ignore them if they decide it is
a scientific necessity.
What is the term for the field of study concerned with the philosophical issues
raised by some medical procedures and treatments?
-The 3Rs
-Bioethics
-Animal welfare
-Biotechnology - ANSWER- Bioethics
What is NOT a component of an animal care and use program described in the
Guide?
-Genotyping procedures
-Euthanasia
-Personnel qualifications
-Animal environment and housing - ANSWER- Genotyping Procedures
Which organization accredits laboratory animal facilities that demonstrate that they
are meeting the standards outlined in the Guide and all federal and local
regulations?
-United States Department of Agriculture
-AAALAC International
LATEST 2025-2026 QUESTIONS AND
ANSWERS 200 REAL EXAM QUESTIONS
AND ANSWERS (VERIFIED ANSWERS)
Which of the following species is covered by the Animal Welfare Act?
-Birds bred specifically for research
-Zebrafish
-Mice
-Hamsters - ANSWER- Hamsters
What types of euthanasia methods are listed in the AVMA Guidelines for the
Euthanasia of Animals?
-Suitable, conditionally suitable, and unsuitable methods
-Adequate, acceptable, and inadequate methods
-Acceptable, conditionally acceptable, and unacceptable methods
-Approved, tentative, and unapproved methods - ANSWER- Acceptable,
conditionally acceptable, and unacceptable methods
The PHS Policy covers all live vertebrates used or intended for use in research,
training, experimental purposes and biological testing.
-False
-True - ANSWER- True
,Which document on animal research is reviewed and approved by the Institutional
Animal Care and Use Committee?
-Research proposal
-Grant
-Animal use protocol
-Research data report - ANSWER- Animal Use Protocol
What federal agency is responsible for enforcing and administering the Animal
Welfare Regulations?
-Animal and Plant Health Inspection Service
-Food and Drug Administration
-Office of Laboratory Animal Welfare
-AAALAC International - ANSWER- Animal and Plant Health Inspection Service
What is NOT a requirement of the AWA/AWR a researcher must comply with, if
they house covered species?
-Appoint an unaffiliated member to the IACUC.
-Submit an annual report including the number of covered species used.
-Provide dogs with exercise.
-Meet or exceed sanitization standards for both cages and facility. - ANSWER-
Appoint an unaffiliated member to the IACUC.
7. According to the Animal Welfare Regulations, to which official does the
IACUC report?
-The Institutional Official (IO) or Chief Executive Officer (CEO)
-The Chair of the Office of Laboratory Animal Welfare or the Area Director
-The Director of the USDA Animal & Plant Health Inspection Service or Chief
Inspecting Officer
-The Attending Veterinarian or Facility Director - ANSWER- The Institutional
Official (IO) or Chief Executive Officer (CEO)
Which agency is responsible for enforcing the Public Health Service Policy on
Humane Care and Use of Laboratory Animals (PHS Policy)?
-Food and Drug Administration
-Animal and Plant Health Inspection Service
, -Office of Laboratory Animal Welfare
-National Research Council - ANSWER- Office of Laboratory Animal Welfare
What is NOT stated as a requirement for compliance with the US Principles for the
Utilization and Care of Vertebrate Animals Used in Testing, Research and
Training?
-Transportation, use and care of animals should be in accordance with the Animal
Welfare Act.
-Principal investigators should determine where exceptions to these principles are
required and may ignore them if they decide it is a scientific necessity.
-Procedures that cause more than momentary or slight pain must be performed
with appropriate sedation, analgesia and anesthesia.
-Animals that suffer unrelieved pain and distress should be painlessly killed at the
end of the procedure. - ANSWER- Principal investigators should determine where
exceptions to these principles are required and may ignore them if they decide it is
a scientific necessity.
What is the term for the field of study concerned with the philosophical issues
raised by some medical procedures and treatments?
-The 3Rs
-Bioethics
-Animal welfare
-Biotechnology - ANSWER- Bioethics
What is NOT a component of an animal care and use program described in the
Guide?
-Genotyping procedures
-Euthanasia
-Personnel qualifications
-Animal environment and housing - ANSWER- Genotyping Procedures
Which organization accredits laboratory animal facilities that demonstrate that they
are meeting the standards outlined in the Guide and all federal and local
regulations?
-United States Department of Agriculture
-AAALAC International
