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UIUC MCB 150 EXAM 2 Questions and Correct Answers/ Latest Update / Already Graded

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pH Ans: Measurement of proton concentration Glycolysis Ans: Cytosol 6 carbon compound - two 3 carbon compounds (Pyruvate) Net energy: 2 ATP 2 NADH 3 phases of Glycolysis Ans: Energy Investment: Phosphates from 2 ATP attach to glucose - rest of reactions can be exergonic Cleavage Phase: breaks apart fructose 1, 6-biphosphate into 2 glyceraldehyde 3-phosphate Energy Liberation/ Payoff Phase: Yields ATP and NADH Substrate-level phosphorylation Ans: ATP synthesized by enzyme transferring phosphate from phosphorylated molecule to ADP Page | 2 All rights reserved © 2025/ 2026 | Phosphofruktokinase Ans: Catalyzes third step in glycolysis High concentrations of ATP, ATP binds to allosteric site, rendering the enzyme inactive Feedback inhibition Pyruvate Oxydation Ans: Mitochondrial Matrix 2 Pyruvate (3-carbon compound) - 2 Acetyl group (2-carbon compounds) attached to coenzyme A = Acetyl CoA 1 NAD+ reduced into NADH - Storage of energy CO2 Pyruvate Dehydrogenase Ans: Oxidizes (breaks down) pyruvate - removes CO2 Citric Acid Cycle Ans: Mitochondrial Matrix cycle twice for every glucose Page | 3 All rights reserved © 2025/ 2026 | acetyl group removed from accetyl CoA and attached to oxaloacetate (4 carbons) - citric acid (6 carbons) 6 NADH /2 = 3

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UIUC MCB 150
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UIUC MCB 150

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UIUC MCB 150 EXAM 2 Questions and
Correct Answers/ Latest Update / Already
Graded
pH

Ans: Measurement of proton concentration


Glycolysis

Ans: Cytosol
6 carbon compound -> two 3 carbon compounds (Pyruvate)
Net energy: 2 ATP 2 NADH


3 phases of Glycolysis

Ans: Energy Investment: Phosphates from 2 ATP attach to
glucose -> rest of reactions can be exergonic
Cleavage Phase: breaks apart fructose 1, 6-biphosphate into 2
glyceraldehyde 3-phosphate
Energy Liberation/ Payoff Phase: Yields ATP and NADH


Substrate-level phosphorylation

Ans: ATP synthesized by enzyme transferring phosphate from
phosphorylated molecule to ADP

All rights reserved © 2025/ 2026 |

, Page |2



Phosphofruktokinase

Ans: Catalyzes third step in glycolysis
High concentrations of ATP, ATP binds to allosteric site,
rendering the enzyme inactive
Feedback inhibition


Pyruvate Oxydation

Ans: Mitochondrial Matrix
2 Pyruvate (3-carbon compound) -> 2 Acetyl group (2-carbon
compounds) attached to coenzyme A = Acetyl CoA
1 NAD+ reduced into NADH -> Storage of energy
CO2


Pyruvate Dehydrogenase

Ans: Oxidizes (breaks down) pyruvate -> removes CO2


Citric Acid Cycle

Ans: Mitochondrial Matrix
cycle twice for every glucose



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