FACS EXAM QUESTIONS AND ANSWERS
GRADED A+ 2025/2026
What kind of cellular properties can be used to sort a population of cells using FACs? -
ANS All of them
What is the flow buffer made of? - ANS Saline solutions
Why is pressure so important in FACs? - ANS To move both the sample and the flow buffer
through the system
What is the importance of the flow cell? - ANS To create a uniform stream for the samples in
flow buffer
What is the function of the closed-loop nozzle? - ANS Ensure a good fluidic system start up
and shut down procedure
How can you measure the cell size? - ANS Using FSC values
Which of these components is not part of the optics system? - ANS The nozzle
What is the interrogation point? - ANS Where the cells intercept the laser beam trajectory
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
GRADED A+ 2025/2026
What kind of cellular properties can be used to sort a population of cells using FACs? -
ANS All of them
What is the flow buffer made of? - ANS Saline solutions
Why is pressure so important in FACs? - ANS To move both the sample and the flow buffer
through the system
What is the importance of the flow cell? - ANS To create a uniform stream for the samples in
flow buffer
What is the function of the closed-loop nozzle? - ANS Ensure a good fluidic system start up
and shut down procedure
How can you measure the cell size? - ANS Using FSC values
Which of these components is not part of the optics system? - ANS The nozzle
What is the interrogation point? - ANS Where the cells intercept the laser beam trajectory
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.