Test Bank For Benson's Microbiological
Applications Laboratory Manual, 15th
Edition All Chapters
PEA poor growth/no growth - answers organism inhibited by phenylethyl alc, probable
gram - organism
PEA good growth - answers organism is not inhibited by phenylethyl alc, probable
staphylococcus, streptococcus, enterococcus, or lactococcus
5% sheep blood poor growth - answers inhibited by colistin and nalidixic acid, probable
gram -
5% sheep blood good growth - answers not inhibited by colistin and nalidixic acid and
completely hemolyzes RBCs, probable alpha hemolytic staph, strep, or entero
Mannitol Salt poor growth - answers organism inhibited by NaCl, not staph
mannitol salt good growth - answers organism not inhibited by NaCl, staph
Macconkey poor growth - answers organism is inhibited by crystal violet and or bile,
gram +
macconkey good growth - answers organism is not inhibited by crystal violet or bile,
gram +
EMB poor growth - answers organism is inhibited by eosin and methylene blue, gram +
EMB good growth - answers organism is not inhibited by eosin and methylene blue,
gram -
Hektoen poor growth - answers organism is inhibited by bile and or one of the dyes
included, gram +
hektoen good growth - answers organism isnt inhibited by bile or any of the dyes, gram
-
Phenol red broth yellow with bubble - answers fermentation with acid and gas end
products, A/G
Phenol Red broth yellow with no bubble - answers fermentation with acid end products,
no gas produced, A/-
phenol red broth red with no bubble - answers no fermentation, -/-
, phenol red broth pink no bubble - answers degradation of peptone, alkaline end
products, K
phenol red broth - answers will have glucose, lactose, or sucrose
methyl red broth red color - answers mixed acid fermentation, +
methyl red broth no color change - answers no mixed acid fermentation, -
voges-proskauer red - answers 2.3 butanedial fermentation, +
voges-proskauer no color change - answers 2.3 butanedial fermentation (acetoin is not
produced), -
citrate test blue - answers citrate is utilized, +
citrate test no color change with growth - answers citrate is utilized, +
citrate test no change no growth - answers citrate is not utilized, -
amylase test clearing around growth - answers alpha amylase and or oligo 1,6
glucosidase is present, +
amylase test no clearing around growth - answers neither alpha amylase and or oligo
1,6 glucosidase is present, -
filiform - answers dark and opaque with smooth edge
friable - answers crusty
pellicle - answers float to top of surface
flocculent - answers clumped together
simple stain - answers crystal violet, safranin, methylene blue
negative stain - answers determines morphology and cellular arrangement without heat
fixing (background is stained)
do you heat fix capsules - answers no
what color are endospores - answers green
gram - vs gram + - answers - ends red,
+ ends purple
Applications Laboratory Manual, 15th
Edition All Chapters
PEA poor growth/no growth - answers organism inhibited by phenylethyl alc, probable
gram - organism
PEA good growth - answers organism is not inhibited by phenylethyl alc, probable
staphylococcus, streptococcus, enterococcus, or lactococcus
5% sheep blood poor growth - answers inhibited by colistin and nalidixic acid, probable
gram -
5% sheep blood good growth - answers not inhibited by colistin and nalidixic acid and
completely hemolyzes RBCs, probable alpha hemolytic staph, strep, or entero
Mannitol Salt poor growth - answers organism inhibited by NaCl, not staph
mannitol salt good growth - answers organism not inhibited by NaCl, staph
Macconkey poor growth - answers organism is inhibited by crystal violet and or bile,
gram +
macconkey good growth - answers organism is not inhibited by crystal violet or bile,
gram +
EMB poor growth - answers organism is inhibited by eosin and methylene blue, gram +
EMB good growth - answers organism is not inhibited by eosin and methylene blue,
gram -
Hektoen poor growth - answers organism is inhibited by bile and or one of the dyes
included, gram +
hektoen good growth - answers organism isnt inhibited by bile or any of the dyes, gram
-
Phenol red broth yellow with bubble - answers fermentation with acid and gas end
products, A/G
Phenol Red broth yellow with no bubble - answers fermentation with acid end products,
no gas produced, A/-
phenol red broth red with no bubble - answers no fermentation, -/-
, phenol red broth pink no bubble - answers degradation of peptone, alkaline end
products, K
phenol red broth - answers will have glucose, lactose, or sucrose
methyl red broth red color - answers mixed acid fermentation, +
methyl red broth no color change - answers no mixed acid fermentation, -
voges-proskauer red - answers 2.3 butanedial fermentation, +
voges-proskauer no color change - answers 2.3 butanedial fermentation (acetoin is not
produced), -
citrate test blue - answers citrate is utilized, +
citrate test no color change with growth - answers citrate is utilized, +
citrate test no change no growth - answers citrate is not utilized, -
amylase test clearing around growth - answers alpha amylase and or oligo 1,6
glucosidase is present, +
amylase test no clearing around growth - answers neither alpha amylase and or oligo
1,6 glucosidase is present, -
filiform - answers dark and opaque with smooth edge
friable - answers crusty
pellicle - answers float to top of surface
flocculent - answers clumped together
simple stain - answers crystal violet, safranin, methylene blue
negative stain - answers determines morphology and cellular arrangement without heat
fixing (background is stained)
do you heat fix capsules - answers no
what color are endospores - answers green
gram - vs gram + - answers - ends red,
+ ends purple
