MGY250 UNIT 8 QUIZ EXAM
QUESTIONS AND ANSWERS GRADED A+
2025/2026
Which of the following statements are true of the CRISPR system: (select all that apply)
The CRISPR locus is easy to modify using RNA engineering and can be designed to cut any
desired double stranded DNA sequence
A bacterial CRISPR locus consists of repetitive sequences interspaced with viral DNA sequence
The Cas9 enzyme recognizes DNA sequences using a guide RNA
Both the CRISPR locus and 'cas' genes are part of a bacterial immune system - ANS A bacterial
CRISPR locus consists of repetitive sequences interspaced with viral DNA sequence
The Cas9 enzyme recognizes DNA sequences using a guide RNA
Both the CRISPR locus and 'cas' genes are part of a bacterial immune system
According to Dr. van der Kooy, current therapeutic treatment with stem cells include (select all
that apply):
Using skin stem cells to grow sheets of skin that can replace burnt skin
Using Yamanaka factors to reprogram leukaemia cells to pluripotent, non-cancerous cells
Using heart stem cells to regenerate heart tissue damaged by cardiac arrest
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
, Using blood stem cells to replenish blood cells in patients with leukaemia who have had their
native supply blood cells eradicated by chemotherapy - ANS Using skin stem cells to grow
sheets of skin that can replace burnt skin
Using blood stem cells to replenish blood cells in patients with leukaemia who have had their
native supply blood cells eradicated by chemotherapy
Dr. van der Kooy described an experiment performed by Dr. Janet Rossant and colleagues in
which mouse embryonic stem cells expressing green fluorescent protein were injected into a
pseudo-pregnant mouse. Using a "genetic trick" the host cells were segregated to the pre-
placental tissue and only the injected cells were in the inner cell mass, such that the mouse
eventually born was completely green. This experiment demonstrated that:
Embryonic stem cells can generate every cell in the body in vivo
Embryonic stem cells can generate chimeric organisms
Embryonic stem cells can contribute to the placenta
Embryonic stem cells do not interfere with the growth of a mouse - ANS Embryonic stem
cells can generate every cell in the body in vivo
iPS cells can be used to (select all that apply):
Model genetic disease by exploring the effect of a particular mutation on the development of
different tissue types
Test drug toxicity
Model genetic disease by exploring the effect of a particular mutation on the development of a
human
Differentiate into different cell types - ANS Model genetic disease by exploring the effect of a
particular mutation on the development of different tissue types
Test drug toxicity
Differentiate into different cell types
2 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
QUESTIONS AND ANSWERS GRADED A+
2025/2026
Which of the following statements are true of the CRISPR system: (select all that apply)
The CRISPR locus is easy to modify using RNA engineering and can be designed to cut any
desired double stranded DNA sequence
A bacterial CRISPR locus consists of repetitive sequences interspaced with viral DNA sequence
The Cas9 enzyme recognizes DNA sequences using a guide RNA
Both the CRISPR locus and 'cas' genes are part of a bacterial immune system - ANS A bacterial
CRISPR locus consists of repetitive sequences interspaced with viral DNA sequence
The Cas9 enzyme recognizes DNA sequences using a guide RNA
Both the CRISPR locus and 'cas' genes are part of a bacterial immune system
According to Dr. van der Kooy, current therapeutic treatment with stem cells include (select all
that apply):
Using skin stem cells to grow sheets of skin that can replace burnt skin
Using Yamanaka factors to reprogram leukaemia cells to pluripotent, non-cancerous cells
Using heart stem cells to regenerate heart tissue damaged by cardiac arrest
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
, Using blood stem cells to replenish blood cells in patients with leukaemia who have had their
native supply blood cells eradicated by chemotherapy - ANS Using skin stem cells to grow
sheets of skin that can replace burnt skin
Using blood stem cells to replenish blood cells in patients with leukaemia who have had their
native supply blood cells eradicated by chemotherapy
Dr. van der Kooy described an experiment performed by Dr. Janet Rossant and colleagues in
which mouse embryonic stem cells expressing green fluorescent protein were injected into a
pseudo-pregnant mouse. Using a "genetic trick" the host cells were segregated to the pre-
placental tissue and only the injected cells were in the inner cell mass, such that the mouse
eventually born was completely green. This experiment demonstrated that:
Embryonic stem cells can generate every cell in the body in vivo
Embryonic stem cells can generate chimeric organisms
Embryonic stem cells can contribute to the placenta
Embryonic stem cells do not interfere with the growth of a mouse - ANS Embryonic stem
cells can generate every cell in the body in vivo
iPS cells can be used to (select all that apply):
Model genetic disease by exploring the effect of a particular mutation on the development of
different tissue types
Test drug toxicity
Model genetic disease by exploring the effect of a particular mutation on the development of a
human
Differentiate into different cell types - ANS Model genetic disease by exploring the effect of a
particular mutation on the development of different tissue types
Test drug toxicity
Differentiate into different cell types
2 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
