MGY250 UNIT 1 - PERSPECTIVES ON
MEDICAL GENETICS EXAM
QUESTIONS AND ANSWERS GRADED A+
2025/2026
Describe how ethical guideliens for CRISPR have been developed - ANS Summit Dec 2015 in
washington DC
DNA Sequencing prep - ANS 1. Cut DNA into small pieces and insert into plasmid DNA for
bacteria to replicate it
2. Isolate DNA from bacteria and trasnsfer to plate
3. Add free DNA bases, DNAP, primers and terminator bases
Define hierarchical shotgun sequencing - ANS - Break up large piece of DNA from genome
into small bits and put it back together
- Assembly relied on Human Genome for reference
- Hierarchical uses smaller pieces to start with
Define DNA cloning - ANS Place DNA into plasmids and allow bacteria to replicate the
plasmids along with your DNA
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
MEDICAL GENETICS EXAM
QUESTIONS AND ANSWERS GRADED A+
2025/2026
Describe how ethical guideliens for CRISPR have been developed - ANS Summit Dec 2015 in
washington DC
DNA Sequencing prep - ANS 1. Cut DNA into small pieces and insert into plasmid DNA for
bacteria to replicate it
2. Isolate DNA from bacteria and trasnsfer to plate
3. Add free DNA bases, DNAP, primers and terminator bases
Define hierarchical shotgun sequencing - ANS - Break up large piece of DNA from genome
into small bits and put it back together
- Assembly relied on Human Genome for reference
- Hierarchical uses smaller pieces to start with
Define DNA cloning - ANS Place DNA into plasmids and allow bacteria to replicate the
plasmids along with your DNA
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.