MLS 4117 FINAL EXAM QUESTIONS AND
ANSWERS GRADED A+ 2025/2026
Transport media for Bordatella pertussis - ANS Regan-Lowe transport media
Transport media for Campylobacter spp. - ANS Cary-Blair
Transport media for Corynebacterium diptheriae - ANS Loeffler or Blood Slant
Transport media for Escherichia coli (hemorrhagic or HUS) - ANS TSI, Nutrient Agar Slant,
Blood slant, TSA slant
Transport media for E. coli O157:H7 - ANS TSI, Nutrient Agar Slant, Blood slant, TSA slant
Transport media for Haemophilus influenzae - ANS Chocolate slant
Transport media for Listeria monocytogenes - ANS Blood slant
Transport media for Mycobacterium spp. - ANS Lowenstein-Jensen or Middlebrook agar
Transport media for Vibrio spp. - ANS TSI, Nutrient Agar Slant, Blood slant, TSA slant
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,Transport media for VRE - ANS Blood, chocolate, or nutrient agar slant
Transport media for Yersinia enterocolitica - ANS TSI, Nutrient Agar Slant, Blood slant, TSA
slant
Transport media for Neisseria meningitidis - ANS Chocolate or blood slant
Transport media for Salmonella spp. - ANS TSI, Nutrient Agar Slant, Blood slant, TSA slant
Transport media for Shigella spp. - ANS TSI, Nutrient Agar Slant, Blood slant, TSA slant
Transport media for Streptococcus pneumoniae - ANS Blood or chocolate slant
Non-motile, Gram - coccobacillus, Catalase+, Oxidase -, MAC+ at 30-35C, TSIA (K/K); nosocomial
hospital equipment, vent-associated pneumonia and sepsis, bronchitis, endocarditis, UTI, Eye
Two most common clinical species are A. baumanii and A. lwoffii
A. baumannii causes more infection than A. lwoffii
A. baumannii is saccharolytic and A. lwoffii is asacharolytic
A. lwoffii more susceptible to antibiotics than A. baumannii
oxidase -, catalase +, non-motile, purple hue on MAC - ANS Acinetobacter
Motile, Gram - rods, purplish green on SBA, Catalase+, Oxidase -, Esculin+, DNAse+, Gelatin
hydrolysis+, MAC (clear/blue), TSIA (K/K), nosocomial health equipment, found in food;
bronchitis, endocarditism eye, meningitis, common in CF; risk for immunosuppression, ICU,
central venous cath
normal flora, SXT (S) - ANS Stenotrophomonas maltophila
2 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
, Gram+ cocci, B-hemolytic, ferments mannitol (MAC/MSA+, yellow), Coag+, Catalase+, Oxidase -,
Bacitracin (R); Food poisoning, conjunctivitis, TSS; leucocidin, Protein A, Non-spore, Non-Motile
- ANS staph aureus
Intracellular, encapsulated, Gram - diplococcus, Cat +, Ox +, Penicillin (S), glu + malt
fermentation, TSIA (K/K), meningitis, septicemia, grown in 5% CO2, SBA and CHOCALATE. CSF
and Blood or Skin lesions or symptoms. - ANS neisseria meningitidis
Pleomorphic non-gram staining molicutes, slow growth, "Fried egg" shape with center growing
into agar, strict aerobe, Cold agglutinin test +' 20% of pneumonias, pharyngitis, "Walking
pneumonia";most common cause of pneumonia in young adults, smallest free living organism,
lack cell walls, aerobe, - ANS mycoplasma pneumoniae
Encapsulated, gram - coccobacillus (pleomorphic), Catalase +, Oxidase +, Nit Red+, SBA -, CHOC
+, Factor V & X req'd, serotype B most common (vaccine), septicemia, meningitis in children,
cellulitis, pneumonia, conjunctivitis. Used to be predominate cause of meningitis in children
between 3 and 6. Acute epiglottitis. non-spore, non-motile, 5% CO2 35-37C, -
ANS haemophilus influenzae
Gram - coccobacilli, "Hockey puck" appearance, Catalase+, Oxidase+, Nit Red +, O-F Media
Green/Green (asaccharolytic), Strict aerobe, SBA+ at 25C, CHOC -, opportunistic/nosocomial
lung infections. DNAse +, NON-FERMENTATIVE. BETA-LACTAMASE +, resistant to ampicillin and
amoxicillin. (s) to tetracycline
Does NOT grow on MAC - ANS moraxella catarhallis
Encapsulated, anaerobic, Gram - rods, Bile (R), Penicillin (R), Metronidazole (S), most common
anaerobic infection; abdominal abscess, wounds, blood infections, normal flora of gut -
ANS bacteroides fragilis
3 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
ANSWERS GRADED A+ 2025/2026
Transport media for Bordatella pertussis - ANS Regan-Lowe transport media
Transport media for Campylobacter spp. - ANS Cary-Blair
Transport media for Corynebacterium diptheriae - ANS Loeffler or Blood Slant
Transport media for Escherichia coli (hemorrhagic or HUS) - ANS TSI, Nutrient Agar Slant,
Blood slant, TSA slant
Transport media for E. coli O157:H7 - ANS TSI, Nutrient Agar Slant, Blood slant, TSA slant
Transport media for Haemophilus influenzae - ANS Chocolate slant
Transport media for Listeria monocytogenes - ANS Blood slant
Transport media for Mycobacterium spp. - ANS Lowenstein-Jensen or Middlebrook agar
Transport media for Vibrio spp. - ANS TSI, Nutrient Agar Slant, Blood slant, TSA slant
1 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
,Transport media for VRE - ANS Blood, chocolate, or nutrient agar slant
Transport media for Yersinia enterocolitica - ANS TSI, Nutrient Agar Slant, Blood slant, TSA
slant
Transport media for Neisseria meningitidis - ANS Chocolate or blood slant
Transport media for Salmonella spp. - ANS TSI, Nutrient Agar Slant, Blood slant, TSA slant
Transport media for Shigella spp. - ANS TSI, Nutrient Agar Slant, Blood slant, TSA slant
Transport media for Streptococcus pneumoniae - ANS Blood or chocolate slant
Non-motile, Gram - coccobacillus, Catalase+, Oxidase -, MAC+ at 30-35C, TSIA (K/K); nosocomial
hospital equipment, vent-associated pneumonia and sepsis, bronchitis, endocarditis, UTI, Eye
Two most common clinical species are A. baumanii and A. lwoffii
A. baumannii causes more infection than A. lwoffii
A. baumannii is saccharolytic and A. lwoffii is asacharolytic
A. lwoffii more susceptible to antibiotics than A. baumannii
oxidase -, catalase +, non-motile, purple hue on MAC - ANS Acinetobacter
Motile, Gram - rods, purplish green on SBA, Catalase+, Oxidase -, Esculin+, DNAse+, Gelatin
hydrolysis+, MAC (clear/blue), TSIA (K/K), nosocomial health equipment, found in food;
bronchitis, endocarditism eye, meningitis, common in CF; risk for immunosuppression, ICU,
central venous cath
normal flora, SXT (S) - ANS Stenotrophomonas maltophila
2 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
, Gram+ cocci, B-hemolytic, ferments mannitol (MAC/MSA+, yellow), Coag+, Catalase+, Oxidase -,
Bacitracin (R); Food poisoning, conjunctivitis, TSS; leucocidin, Protein A, Non-spore, Non-Motile
- ANS staph aureus
Intracellular, encapsulated, Gram - diplococcus, Cat +, Ox +, Penicillin (S), glu + malt
fermentation, TSIA (K/K), meningitis, septicemia, grown in 5% CO2, SBA and CHOCALATE. CSF
and Blood or Skin lesions or symptoms. - ANS neisseria meningitidis
Pleomorphic non-gram staining molicutes, slow growth, "Fried egg" shape with center growing
into agar, strict aerobe, Cold agglutinin test +' 20% of pneumonias, pharyngitis, "Walking
pneumonia";most common cause of pneumonia in young adults, smallest free living organism,
lack cell walls, aerobe, - ANS mycoplasma pneumoniae
Encapsulated, gram - coccobacillus (pleomorphic), Catalase +, Oxidase +, Nit Red+, SBA -, CHOC
+, Factor V & X req'd, serotype B most common (vaccine), septicemia, meningitis in children,
cellulitis, pneumonia, conjunctivitis. Used to be predominate cause of meningitis in children
between 3 and 6. Acute epiglottitis. non-spore, non-motile, 5% CO2 35-37C, -
ANS haemophilus influenzae
Gram - coccobacilli, "Hockey puck" appearance, Catalase+, Oxidase+, Nit Red +, O-F Media
Green/Green (asaccharolytic), Strict aerobe, SBA+ at 25C, CHOC -, opportunistic/nosocomial
lung infections. DNAse +, NON-FERMENTATIVE. BETA-LACTAMASE +, resistant to ampicillin and
amoxicillin. (s) to tetracycline
Does NOT grow on MAC - ANS moraxella catarhallis
Encapsulated, anaerobic, Gram - rods, Bile (R), Penicillin (R), Metronidazole (S), most common
anaerobic infection; abdominal abscess, wounds, blood infections, normal flora of gut -
ANS bacteroides fragilis
3 @COPYRIGHT 2025/2026 ALLRIGHTS RESERVED.
