temperture dependent
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for turtles, low temperatures produce all males, intermediate temperatures
produce 50-50, and warmer temperatures produce all females, Dmrt1 is
produced/expressed at high levels at the male promoting temperature
Wolfian ducts
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Will be become internal male reproductive structures if activated by the
SRY gene
,indels
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insertions and deletions
plasmids
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circular pieces of DNA that can replicate in bacteria independently of the
chromosome, about 4000 bp in size, need an origin of replication
More accurate sequencing
Give this one a try later!
pac bio (10-20,000 bp), oxford nanopore (100,000-1 mil bp)
Next generation sequencing (illumina)
Give this one a try later!
, millions of DNA fragments sequenced in parallel: take tisuue from organism
and extract DNA, break DNA into small 200-500 bp fragments, ligate
adapters to the ends of fragments, fragments are amplified to create 100-
200 million clusters
melanin
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the main pigments determining mammalian skin and hair color, is
synthesized from enzymes , intermediates are eventually converted into 1
of 2 types
recombinant DNA
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DNA produced by combining DNA from different sources, pieces of DNA
cut with the same restriction endonuclease can be joined together to
produce recombinant DNA
-blast
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undifferntiated version of something
, Oligo(dT) primer
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latches onto polyA tail of the mRNA, acting as its primer
reversible dye terminators
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use nucleotides similar to dideoxynucleotides that block strand elongation.
Includes fluorophore with a color corresponding to the base pair (A, C, G,
T), terminators get added to strand by DNA polymerase, can be chemically
removed to continue strand elongation, DNTps not used
Transformation of plants by Agrobaterium tumefaciens
Give this one a try later!
bacteria have evolved a way to insert DNA into plant genome and cause it
to build a tumor which is a good environment for plant growth
Y chromsome size
Give this one a try later!
Give this one a try later!
for turtles, low temperatures produce all males, intermediate temperatures
produce 50-50, and warmer temperatures produce all females, Dmrt1 is
produced/expressed at high levels at the male promoting temperature
Wolfian ducts
Give this one a try later!
Will be become internal male reproductive structures if activated by the
SRY gene
,indels
Give this one a try later!
insertions and deletions
plasmids
Give this one a try later!
circular pieces of DNA that can replicate in bacteria independently of the
chromosome, about 4000 bp in size, need an origin of replication
More accurate sequencing
Give this one a try later!
pac bio (10-20,000 bp), oxford nanopore (100,000-1 mil bp)
Next generation sequencing (illumina)
Give this one a try later!
, millions of DNA fragments sequenced in parallel: take tisuue from organism
and extract DNA, break DNA into small 200-500 bp fragments, ligate
adapters to the ends of fragments, fragments are amplified to create 100-
200 million clusters
melanin
Give this one a try later!
the main pigments determining mammalian skin and hair color, is
synthesized from enzymes , intermediates are eventually converted into 1
of 2 types
recombinant DNA
Give this one a try later!
DNA produced by combining DNA from different sources, pieces of DNA
cut with the same restriction endonuclease can be joined together to
produce recombinant DNA
-blast
Give this one a try later!
undifferntiated version of something
, Oligo(dT) primer
Give this one a try later!
latches onto polyA tail of the mRNA, acting as its primer
reversible dye terminators
Give this one a try later!
use nucleotides similar to dideoxynucleotides that block strand elongation.
Includes fluorophore with a color corresponding to the base pair (A, C, G,
T), terminators get added to strand by DNA polymerase, can be chemically
removed to continue strand elongation, DNTps not used
Transformation of plants by Agrobaterium tumefaciens
Give this one a try later!
bacteria have evolved a way to insert DNA into plant genome and cause it
to build a tumor which is a good environment for plant growth
Y chromsome size
Give this one a try later!
