LECOM Seton Hill Histology Exam 1
Stains Questions And Answers
/. Periodic Acid-Schiff - Answer-✅Magenta: glycogen and carb-rich molecules, tests for
Von Gierke's.
/.Haemotoxylin - Answer-✅Purple, stains acids.
/.Eosin - Answer-✅Pink, stains bases.
/.Prussian Blue - Answer-✅Blue, stains iron molecules
/.Congo Red - Answer-✅Red, stain amyloid proteins in beta-pleated sheets like prions.
Plane polarized light can see greens?
/.Trichrome - Answer-✅Red, blue/green, and yellow. Stains collagen fibers
/.Reticulin - Answer-✅Can be silver. Dyes reticulin fibers (type III collagen).
/.DAB - Answer-✅Immunochemistry Ab. Binds targeted Ab and releases light.
/.Cytokeratin - Answer-✅Immunochemistry for Epithelial cells.
/.Vimentin - Answer-✅Immunochemistry for mesenchymal cells.
/.Desmin - Answer-✅Immunohistochemical stain for Muscle.
/.Prostate Specific Antigen - Answer-✅Immunohistochemical stain for prostate
epithelium.
/.HER2/neu - Answer-✅Immunohistochemical stain for breast cancer (carcinomas). Cell
membrane
/.p53 - Answer-✅Stains cancerous nuclei.
/.Von Gierke's disease - Answer-✅glycogen storage disease (G6Pase deficiency)
/.Progeria - Answer-✅Rapid aging caused by Nuclear Lamin A mutant
, /.Smooth ER (Detoxification) - Answer-✅Hydroxylation rxn, conjugation rxn, makes
toxin water soluble and excrete-able
/.I-cell disease - Answer-✅No Mannose-6-P added to proteins, lysosomal storage
disease
/.Hyperproinsulinemia - Answer-✅insulin protein not cleaved properly, presents as
diabetic bc insulin not functioning
/.Gaucher disease - Answer-✅Glucocerebroside accumulation due to enzyme mutation
/.Zellweger syndrome - Answer-✅Defective peroxisome biogenesis = defective
proxisomal oxidation. Inability to breakdown LC FAs due to peroxisome defect. FAs are
cut short and sent to mitochondria. hepatomeg, Mental Retardation, Infantile death.
Severe
/.mitochondrial myopathy - Answer-✅disease of the muscle due to mitochondria not
functioning correctly
/.microtubules - Answer-✅tubulin, biggest of three, chromosomal movement and
intracellular vesicle and organelle transport, kinesin, dynein, associated with herpes
/.intermediate filaments - Answer-✅middle size, cytokeratines( epithelial) Desmin (
smooth muscle), Vimentin (mesenchymal organs) GFA (astrocytes)
/.Microfilaments - Answer-✅smallest, actin, sliding movement and polymerization
movement
/.simple squamous epithelium - Answer-✅diffusion and filtration, lines blood vessels
endothelium, and serous membrane (mesothelium), loop of henle
/.simple cuboidal epithelium - Answer-✅secretion and absorption, lines ducts of glands,
walls of kidney tubules
/.pseudostratified columnar epithelium - Answer-✅tissue that consists of a single layer
of irregularly shaped and sized cells that give the appearance of multiple layers; found
in ducts of certain glands and the upper respiratory tract
/.Primary cilia diskenesia - Answer-✅Immotile cilia syndrome due to absent dynein
arms
/.Occulding junctions - Answer-✅Tight junction at top of lateral border, occludins and
claudins
Stains Questions And Answers
/. Periodic Acid-Schiff - Answer-✅Magenta: glycogen and carb-rich molecules, tests for
Von Gierke's.
/.Haemotoxylin - Answer-✅Purple, stains acids.
/.Eosin - Answer-✅Pink, stains bases.
/.Prussian Blue - Answer-✅Blue, stains iron molecules
/.Congo Red - Answer-✅Red, stain amyloid proteins in beta-pleated sheets like prions.
Plane polarized light can see greens?
/.Trichrome - Answer-✅Red, blue/green, and yellow. Stains collagen fibers
/.Reticulin - Answer-✅Can be silver. Dyes reticulin fibers (type III collagen).
/.DAB - Answer-✅Immunochemistry Ab. Binds targeted Ab and releases light.
/.Cytokeratin - Answer-✅Immunochemistry for Epithelial cells.
/.Vimentin - Answer-✅Immunochemistry for mesenchymal cells.
/.Desmin - Answer-✅Immunohistochemical stain for Muscle.
/.Prostate Specific Antigen - Answer-✅Immunohistochemical stain for prostate
epithelium.
/.HER2/neu - Answer-✅Immunohistochemical stain for breast cancer (carcinomas). Cell
membrane
/.p53 - Answer-✅Stains cancerous nuclei.
/.Von Gierke's disease - Answer-✅glycogen storage disease (G6Pase deficiency)
/.Progeria - Answer-✅Rapid aging caused by Nuclear Lamin A mutant
, /.Smooth ER (Detoxification) - Answer-✅Hydroxylation rxn, conjugation rxn, makes
toxin water soluble and excrete-able
/.I-cell disease - Answer-✅No Mannose-6-P added to proteins, lysosomal storage
disease
/.Hyperproinsulinemia - Answer-✅insulin protein not cleaved properly, presents as
diabetic bc insulin not functioning
/.Gaucher disease - Answer-✅Glucocerebroside accumulation due to enzyme mutation
/.Zellweger syndrome - Answer-✅Defective peroxisome biogenesis = defective
proxisomal oxidation. Inability to breakdown LC FAs due to peroxisome defect. FAs are
cut short and sent to mitochondria. hepatomeg, Mental Retardation, Infantile death.
Severe
/.mitochondrial myopathy - Answer-✅disease of the muscle due to mitochondria not
functioning correctly
/.microtubules - Answer-✅tubulin, biggest of three, chromosomal movement and
intracellular vesicle and organelle transport, kinesin, dynein, associated with herpes
/.intermediate filaments - Answer-✅middle size, cytokeratines( epithelial) Desmin (
smooth muscle), Vimentin (mesenchymal organs) GFA (astrocytes)
/.Microfilaments - Answer-✅smallest, actin, sliding movement and polymerization
movement
/.simple squamous epithelium - Answer-✅diffusion and filtration, lines blood vessels
endothelium, and serous membrane (mesothelium), loop of henle
/.simple cuboidal epithelium - Answer-✅secretion and absorption, lines ducts of glands,
walls of kidney tubules
/.pseudostratified columnar epithelium - Answer-✅tissue that consists of a single layer
of irregularly shaped and sized cells that give the appearance of multiple layers; found
in ducts of certain glands and the upper respiratory tract
/.Primary cilia diskenesia - Answer-✅Immotile cilia syndrome due to absent dynein
arms
/.Occulding junctions - Answer-✅Tight junction at top of lateral border, occludins and
claudins
