Biochemistry ACS Final
What is the Henderson-Hasselbach ? - Answer-pH = pka + log ([A]/[Ha])
High Ka, low pka indicates what? - Answer-Strong acid
What is the relation between pH and [H+]? - Answer-pH = -log[H+]
What is the realtionship between pka and Ka? - Answer-pka = -logKa
What makes a good buffer? - Answer-A weak acid mixed with its conjugate base in a 50/50 ratio
What destroys tryptophan? - Answer-Acids and bases
What is the isoelectric point? - Answer-The pH in which a particular molecule carries no net charge (pI)
Side chain pka of aspartic acid? - Answer-~ 3.86
Side chain pka of arginine? - Answer-~ 12.48
Side chain pka of cysteine? - Answer-~8.0
, Side chain pka of glutamic acid? - Answer-~4.07
Side chain pka of histidine? - Answer-~6.1
Side chain pka of lysine? - Answer-~10.53
Side chain pka of tyrosine? - Answer-~10.00
Inductive effect on pka? - Answer-Electronegative atoms pull electrons away from another group, and
makes that particular groups more positive. Generally lowers pka
Electrostatic effect on pka? - Answer-Nearby positive charge makes a proton want to leave more,
generally lowers pka.
Distance effect on pka? - Answer-Generally lowers the influence of inductive and electrostatic effects
How are amino acids read? - Answer-N terminus to C terminus
How to find specific activity? - Answer-mols of pdt / min / mg of protein
What does specific activity measure? - Answer-It measures the purity of the protein
What happens near a protein's isoelectric point? - Answer-The protein loses solubility
How to separate proteins by size (all or none)? - Answer-1. Filtration
2. Dialysis
What is the Henderson-Hasselbach ? - Answer-pH = pka + log ([A]/[Ha])
High Ka, low pka indicates what? - Answer-Strong acid
What is the relation between pH and [H+]? - Answer-pH = -log[H+]
What is the realtionship between pka and Ka? - Answer-pka = -logKa
What makes a good buffer? - Answer-A weak acid mixed with its conjugate base in a 50/50 ratio
What destroys tryptophan? - Answer-Acids and bases
What is the isoelectric point? - Answer-The pH in which a particular molecule carries no net charge (pI)
Side chain pka of aspartic acid? - Answer-~ 3.86
Side chain pka of arginine? - Answer-~ 12.48
Side chain pka of cysteine? - Answer-~8.0
, Side chain pka of glutamic acid? - Answer-~4.07
Side chain pka of histidine? - Answer-~6.1
Side chain pka of lysine? - Answer-~10.53
Side chain pka of tyrosine? - Answer-~10.00
Inductive effect on pka? - Answer-Electronegative atoms pull electrons away from another group, and
makes that particular groups more positive. Generally lowers pka
Electrostatic effect on pka? - Answer-Nearby positive charge makes a proton want to leave more,
generally lowers pka.
Distance effect on pka? - Answer-Generally lowers the influence of inductive and electrostatic effects
How are amino acids read? - Answer-N terminus to C terminus
How to find specific activity? - Answer-mols of pdt / min / mg of protein
What does specific activity measure? - Answer-It measures the purity of the protein
What happens near a protein's isoelectric point? - Answer-The protein loses solubility
How to separate proteins by size (all or none)? - Answer-1. Filtration
2. Dialysis
