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BIO 3302 SESSION 2 QUESTIONS WITH COMPLETE ANSWERS

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BIO 3302 SESSION 2 QUESTIONS WITH COMPLETE ANSWERS

Institution
BIO 3302
Course
BIO 3302

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When do we use a needle and when do we use a loop?


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LOOP is for LIQUID, NEEDLE is for solid




Were your cultures consistent in appearance? How could you confirm your culture
was a pure culture? Where during the process of aseptic transfers could contaminants

, be introduced?


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Yes, the cultures were consistent in appearance. The culture was pure
because they had the same color, transparency, elevation and edge. The
test tube cap is a source of contamination as well as not sterilizing the loop
before and after transfer




Describe and draw the growth of your organism on the Petri dish; be sure to include
the organism' names


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Micrococcus luteus; yellow color with smooth, buttery texture




In the case file, the organism responsible for the patient's death is a normal member
of the human microbiota. How do you explain it causing the death of this patient?


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It was introduced into an area where the microbiota is not found. The
microbiota is normally found in the oral cavity and intestinal tract




Jeffrey, Jane's partner, inoculated a tryptone broth tube with E. coli. When he
examined his broth tube during the next lab period, there was no growth at all. What
could have gone wrong with his experiment?


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Institution
BIO 3302
Course
BIO 3302

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