List at least two factors that can affect the results of the gram staining procedure.
Give this one a try later!
Too much water (will not evaporate) on slide so smear doesn't air-dry. Too
much inoculum (comma-size scraping); Not-heat-fixing the slide and
bacteria wipe off; Using too much decolorizer
, Know where and how mistakes can be made.
Give this one a try later!
using safranin instead of crystal violet in the first step, all bacteria will be
purple because safranin cannot form a complex with Gram's iodine
not heat-fixing the bacteria to the slide, the bacteria will wash off the slide
leaving the slide in the alcohol-acetone for too long, you will wash away
the crystal violet on gram-positive bacteria as well
You mistakenly confuse the primary stain and counterstain. You initially stain the smear
with safranin, add iodine, and then decolorize and counterstain with crystal violet.
How does your mixed colony now appear when viewed with oil immersion?
Give this one a try later!
The colony will appear purple. The safranin and iodine cannot form a
complex. So it will be washed away with the acetone-alcohol. When the
crystal violet is added, all gram positive and gram negative bacteria will
appear purple
What is the counterstain for the Gram staining procedure?
Give this one a try later!
Safranin
Give this one a try later!
Too much water (will not evaporate) on slide so smear doesn't air-dry. Too
much inoculum (comma-size scraping); Not-heat-fixing the slide and
bacteria wipe off; Using too much decolorizer
, Know where and how mistakes can be made.
Give this one a try later!
using safranin instead of crystal violet in the first step, all bacteria will be
purple because safranin cannot form a complex with Gram's iodine
not heat-fixing the bacteria to the slide, the bacteria will wash off the slide
leaving the slide in the alcohol-acetone for too long, you will wash away
the crystal violet on gram-positive bacteria as well
You mistakenly confuse the primary stain and counterstain. You initially stain the smear
with safranin, add iodine, and then decolorize and counterstain with crystal violet.
How does your mixed colony now appear when viewed with oil immersion?
Give this one a try later!
The colony will appear purple. The safranin and iodine cannot form a
complex. So it will be washed away with the acetone-alcohol. When the
crystal violet is added, all gram positive and gram negative bacteria will
appear purple
What is the counterstain for the Gram staining procedure?
Give this one a try later!
Safranin
